This study describes Crenosoma brasiliense (Nematoda, Metastrongyloidea), a new species parasitic in bronchi and bronchioles of Galictis cuja (Molina) (Carnivora, Mustelidae) from Brazil. This species differs from other 11 species of Crenosoma by having a cuticular projection at the distal end of the spicules, forming a prominent blade at the tip of the spicule, a vulval cuticular appendage with a triangular shape and prominent vulval lips. There are no previous records of species of Metastrongyloidea in G. cuja or species of Crenosoma in South America. Therefore, the new species represents the first host record and first geographical record of species of Crenosoma in South America.

• MeSH
• bronchy parazitologie   MeSH
• cestodózy epidemiologie   parazitologie   veterinární   MeSH
• druhová specificita MeSH
• Metastrongyloidea anatomie a histologie   klasifikace   izolace a purifikace   MeSH
• Mustelidae parazitologie   MeSH
• plicní nemoci parazitologie   veterinární   MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Brazílie epidemiologie   MeSH

The Javan ferret-badger Melogale orientalis (Carnivora: Mustelidae: Helictidinae) is a small carnivore endemic to Indonesia. In the family Mustelidae, 10 Eimeria, 12 Cystoisopora, one Isospora, and one Hammondia species are known, but no eimeriid coccidia has been yet described in the subfamily Helictinidae (ferret badgers). Coproscopic examination of Javan ferret-badgers imported into the Czech Republic revealed the presence of coccidian oocysts. Sporulated oocysts differ from other Eimeria known in the family Mustelidae by their small size (12.4-16.1 × 10.4-13.4 μm) and ovoidal shape. Morphological data and phylogenetic analyses of 18S rRNA and COI genes indicated a new species of Eimeria found in faecal samples of Javan ferret badgers. The species is described as E. melogale n. sp.

• Klíčová slova
• Carnivora, Coccidia, Mustelidae, Phylogeny,
• MeSH
• druhová specificita MeSH
• Eimeria klasifikace   cytologie   genetika   MeSH
• feces parazitologie   MeSH
• fylogeneze * MeSH
• Mustelidae parazitologie   MeSH
• oocysty cytologie   MeSH
• RNA ribozomální 18S genetika   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Česká republika MeSH
• Názvy látek
• RNA ribozomální 18S MeSH

Detailed clinical and radiographical descriptions of oral/dental pathology in the beech marten (Martes foina) are lacking. In the present study, skulls of M. foina from a museum collection (n = 109) were examined macroscopically according to predefined criteria and full-mouth dental radiographs were obtained. Occlusion was diagnosed as 'scissor bite' in 22.5% of evaluated skulls, 77% of evaluated skulls showed 'level bite' of incisors and 9.2% of evaluated skulls had 'open bites'. In three skulls, class III malocclusion was diagnosed. Linguoversion of the mandibular second incisor teeth was noted in all skulls. Of the maximum possible number of teeth (i.e. full set of teeth in each skull), 90.4% were available for examination, 6.4% were missing artifactually, 2.0% were absent presumably congenitally and in 1.2 % the absence was presumably acquired. In three skulls supernumerary teeth were noted, all being incisor teeth. In four skulls, unerupted maxillary canine teeth were noted and in one skull odontodysplasia of the maxillary canine teeth was diagnosed. All other teeth were considered normal in morphology. The number of roots per tooth varied in the mandibular second premolar, maxillary first molar and mandibular second molar teeth. The most common dental pathology was attrition/abrasion with 72 skulls (66%) and 857 teeth (24.1% of evaluated teeth) affected, followed by periodontal disease affecting 52 (47.7%) of skulls and 773 teeth (18.9% of evaluated teeth). Dental fractures were present in 49 (45%) skulls and 148 teeth (3.8% of evaluated teeth). Radiographically evident periapical lesions were detected in 11 (10.1%) skulls and 18 teeth (0.5% of evaluated teeth). Other rare abnormal findings included tooth resorption, enamel hypoplasia/hypocalcification, fenestrations at palatal root of maxillary first molar tooth and different bony changes.

• Klíčová slova
• beech martin, oral pathology, radiography, teeth,
• MeSH
• diagnóza stomatologická MeSH
• Mustelidae MeSH
• nemoci parodontu patologie   veterinární   MeSH
• nemoci zubů patologie   veterinární   MeSH
• zuby patologie   MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

BACKGROUND: The mammalian Leukocyte Receptor Complex (LRC) chromosomal region may contain gene families for the killer cell immunoglobulin-like receptor (KIR) and/or leukocyte immunoglobulin-like receptor (LILR) collections as well as various framing genes. This complex region is well described in humans, mice, and some domestic animals. Although single KIR genes are known in some Carnivora, their complements of LILR genes remain largely unknown due to obstacles in the assembly of regions of high homology in short-read based genomes. METHODS: As part of the analysis of felid immunogenomes, this study focuses on the search for LRC genes in reference genomes and the annotation of LILR genes in Felidae. Chromosome-level genomes based on single-molecule long-read sequencing were preferentially sought and compared to representatives of the Carnivora. RESULTS: Seven putatively functional LILR genes were found across the Felidae and in the Californian sea lion, four to five genes in Canidae, and four to nine genes in Mustelidae. They form two lineages, as seen in the Bovidae. The ratio of functional genes for activating LILRs to inhibitory LILRs is slightly in favor of inhibitory genes in the Felidae and the Canidae; the reverse is seen in the Californian sea lion. This ratio is even in all of the Mustelidae except the Eurasian otter, which has a predominance of activating LILRs. Various numbers of LILR pseudogenes were identified. CONCLUSIONS: The structure of the LRC is rather conservative in felids and the other Carnivora studied. The LILR sub-region is conserved within the Felidae and has slight differences in the Canidae, but it has taken various evolutionary paths in the Mustelidae. Overall, the process of pseudogenization of LILR genes seems to be more frequent for activating receptors. Phylogenetic analysis found no direct orthologues across the Carnivora which corroborate the rapid evolution of LILRs seen in mammals.

• Klíčová slova
• KIR, LILR, Leukocyte Receptor Complex, carnivora, felids, long-read sequencing,
• MeSH
• Canidae * MeSH
• Carnivora * genetika   MeSH
• Felidae * MeSH
• fylogeneze MeSH
• genomika MeSH
• lachtani * MeSH
• leukocyty MeSH
• lidé MeSH
• Mustelidae * MeSH
• myši MeSH
• receptory imunologické genetika   MeSH
• receptory KIR genetika   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• receptory imunologické MeSH
• receptory KIR MeSH

Cryptochromes are a ubiquitous group of blue-light absorbing flavoproteins that in the mammalian retina have an important role in the circadian clock. In birds, cryptochrome 1a (Cry1a), localized in the UV/violet-sensitive S1 cone photoreceptors, is proposed to be the retinal receptor molecule of the light-dependent magnetic compass. The retinal localization of mammalian Cry1, homologue to avian Cry1a, is unknown, and it is open whether mammalian Cry1 is also involved in magnetic field sensing. To constrain the possible role of retinal Cry1, we immunohistochemically analysed 90 mammalian species across 48 families in 16 orders, using an antiserum against the Cry1 C-terminus that in birds labels only the photo-activated conformation. In the Carnivora families Canidae, Mustelidae and Ursidae, and in some Primates, Cry1 was consistently labeled in the outer segment of the shortwave-sensitive S1 cones. This finding would be compatible with a magnetoreceptive function of Cry1 in these taxa. In all other taxa, Cry1 was not detected by the antiserum that likely also in mammals labels the photo-activated conformation, although Western blots showed Cry1 in mouse retinal cell nuclei. We speculate that in the mouse and the other negative-tested mammals Cry1 is involved in circadian functions as a non-light-responsive protein.

• MeSH
• antisérum chemie   MeSH
• Canidae fyziologie   MeSH
• čípky retiny - opsiny genetika   MeSH
• čípky retiny fyziologie   účinky záření   ultrastruktura   MeSH
• cirkadiánní rytmus fyziologie   účinky záření   MeSH
• exprese genu MeSH
• fylogeneze * MeSH
• Hominidae fyziologie   MeSH
• imunohistochemie MeSH
• konformace proteinů MeSH
• kryptochromy chemie   genetika   MeSH
• magnetické pole MeSH
• medvědovití fyziologie   MeSH
• Mustelidae fyziologie   MeSH
• proteinové domény MeSH
• ptáci fyziologie   MeSH
• savci klasifikace   fyziologie   MeSH
• světlo MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antisérum MeSH
• čípky retiny - opsiny MeSH
• kryptochromy MeSH

A total of 109 badger Meles meles skulls from Catalonia (north-eastern Iberian Peninsula) were studied for helminths. The tremadode Troglotrema acutum is reported here for the first time in the Eurasian badger in the Iberian Peninsula and southern Europe. Three methodologies were used to detect this trematode: an examination for surface lesions, axial computed tomography and fresh skull dissection. The damage caused in the affected skulls is described, along with details regarding the use of computed tomography to detect hyperostosis, leakage in the sinus structure and bone surface erosion in the affected skulls.

• MeSH
• hyperostóza patologie   MeSH
• lebka diagnostické zobrazování   parazitologie   patologie   MeSH
• Mustelidae parazitologie   MeSH
• počítačová rentgenová tomografie MeSH
• Troglotrematidae klasifikace   izolace a purifikace   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Španělsko MeSH

Examination of the nasolacrimal sinuses of pine marten (Martes martes), stone marten (M. foina) and polecat (Mustela putorius) from 27 localities in the Czech Republic were carried out. In 1 of the 23 stone martens, 6 nematodes (2 males, 4 females) were found and classified as Skrjabingylus petrowi Bageanov (in Petrov 1941). This is the first finding of S. petrowi in central Europe. The morphometry of the specimens in the present study was in agreement with that of the original description. The distribution of this species in Europe is discussed.

• MeSH
• divoká zvířata parazitologie   MeSH
• infekce hlísticemi řádu Strongylida veterinární   MeSH
• Mustelidae parazitologie   MeSH
• Strongylida anatomie a histologie   klasifikace   izolace a purifikace   MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Česká republika MeSH
• Evropa MeSH

The conservation of large carnivores is a formidable challenge for biodiversity conservation. Using a data set on the past and current status of brown bears (Ursus arctos), Eurasian lynx (Lynx lynx), gray wolves (Canis lupus), and wolverines (Gulo gulo) in European countries, we show that roughly one-third of mainland Europe hosts at least one large carnivore species, with stable or increasing abundance in most cases in 21st-century records. The reasons for this overall conservation success include protective legislation, supportive public opinion, and a variety of practices making coexistence between large carnivores and people possible. The European situation reveals that large carnivores and people can share the same landscape.

• MeSH
• biodiverzita MeSH
• lidé MeSH
• Lynx * MeSH
• medvědovití * MeSH
• Mustelidae * MeSH
• vlci * MeSH
• zachování přírodních zdrojů * MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Evropa MeSH

BACKGROUND: Anaplasma phagocytophilum is characterized by a worldwide distribution and distinguished from other Anaplasmataceae by the broadest range of mammalian hosts and high genetic diversity. The role carnivores play in the life cycle of A. phagocytophilum in Europe is uncertain. Currently, only the red fox is considered a suitable reservoir host. In this study, we focused on native and invasive medium-sized carnivore species that live in sympatry and represent the most abundant species of wild carnivores in Poland. METHODS: A total of 275 individual spleen samples from six carnivore species (Vulpes vulpes, Meles meles, Procyon lotor, Nyctereutes procyonoides and Martes spp.) were screened combining nested PCR and sequencing for A. phagocytophilum targeting a partial groEL gene with subsequent phylogenetic analysis inferred by the maximum likelihood method. RESULTS: The DNA of A. phagocytophilum was detected in 16 of 275 individuals (5.8%). Eight unique genetic variants of A. phagocytophilum were obtained. All detected haplotypes clustered in the clade representing European ecotype I. Three variants belonged to the subclade with European human cases together with strains from dogs, foxes, cats, and wild boars. CONCLUSIONS: While carnivores might have a restricted role in the dissemination of A. phagocytophilum due to their relatively low to moderate infection rates, they hold significance as hosts for ticks. Consequently, they could contribute to the transmission of tick-borne infections to humans indirectly, primarily through tick infection. This underscores the potential risk of urbanization for the A. phagocytophilum life cycle, further emphasizing the need for comprehensive understanding of its ecological dynamics.

• Klíčová slova
• Anaplasma phagocytophilum, Carnivores, Invasive species, Martes spp., Meles meles, Nyctereutes procyonides, Procyon lotor, Vulpes vulpes,
• MeSH
• Anaplasma phagocytophilum * genetika   MeSH
• Carnivora * MeSH
• fylogeneze MeSH
• klíšťata * MeSH
• lidé MeSH
• Mustelidae * MeSH
• prasata MeSH
• psi MeSH
• Sus scrofa MeSH
• sympatrie MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• psi MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Polsko epidemiologie   MeSH

The basic information dealing with the anatomy of the ferret's immune system, cross-reactivity of the ferret leukocytes with polyclonal and monoclonal antibodies in vitro and immune response to the mitogens and various infections are presented. The leukocyte numbers in the peripheral blood in the ferrets are lower compared to other species and only one subclass of IgG has been identified in ferrets so far. Lymphocytes make up 12-67% of all the leukocytes in the peripheral blood of the healthy adult ferrets. Lymphocyte subpopulations are similar to other mammals and include T- and B-lymphocytes. T-lymphocytes differentiate into helper (Th) lymphocytes and cytotoxic (Tc) lymphocytes. Currently, ferret granulocytes (CD11), B-lymphocytes (CD79α), T-lymphocytes (CD3), Th-lymphocytes (CD3, CD4), Tc-lymphocytes (CD3, CD8), and CD30, CD45 subpopulations are detected with the use of a number of polyclonal as well as with monoclonal antibodies. In a lymphocyte transformation assay, the mitogen response of the peripheral blood mononuclear cells to concanavalin A (ConA), phytohaemagglutinin (PHA), and pokeweed mitogen (PWM) is the greatest at day 2, 2 and 3, respectively. Serious lymphopenia is observed in ferrets during a distemper infection. A significant decrease in the lymphocyte transformation activity is observed on day 5 and reaches a maximal decrease on days 8-11, with full recovery on days 23-30 after the inoculation of laboratory ferrets with the distemper virus. Ferrets have also been used in studies related to the function of the immune system in Helicobacter pylori infections, Crohn's disease and bronchial asthma.

• Klíčová slova
• Mustelidae, cross-reactive reagents, immune response, immunology,
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH