Nuclear magnetic resonance (MR) imaging provides a noninvasive method for studying the fate of transplanted cells in vivo. We studied, in animals with a cortical photochemical lesion or with a balloon-induced spinal cord compression lesion, the fate of implanted rat bone marrow stromal cells (MSCs) and mouse embryonic stem cells (ESCs) labeled with superparamagnetic iron oxide nanoparticles (Endorem). MSCs were colabeled with bromodeoxyuridine (BrdU), and ESCs were transfected with pEGFP-C1 (eGFP ESCs). Cells were either grafted intracerebrally into the contralateral hemisphere of the adult rat brain or injected intravenously. In vivo MR imaging was used to track their fate; Prussian blue staining and electron microscopy confirmed the presence of iron oxide nanoparticles inside the cells. During the first week postimplantation, grafted cells migrated to the lesion site and populated the border zone of the lesion. Less than 3% of MSCs differentiated into neurons and none into astrocytes; 5% of eGFP ESCs differentiated into neurons, whereas 70% of eGFP ESCs became astrocytes. The implanted cells were visible on MR images as a hypointense area at the injection site, in the corpus callosum and in the lesion. The hypointense signal persisted for more than 50 days. The presence of GFP-positive or BrdU-positive and nanoparticle-labeled cells was confirmed by histological staining. Our study demonstrates that both grafted MSCs and eGFP ESCs labeled with a contrast agent based on iron oxide nanoparticles migrate into the injured CNS. Iron oxide nanoparticles can therefore be used as a marker for the long-term noninvasive MR tracking of implanted stem cells.

• MeSH
• buňky kostní dřeně metabolismus   ultrastruktura   MeSH
• elektronová mikroskopie metody   MeSH
• embryo savčí MeSH
• fosfopyruváthydratasa metabolismus   MeSH
• gliový fibrilární kyselý protein metabolismus   MeSH
• imunohistochemie metody   MeSH
• kmenové buňky metabolismus   ultrastruktura   MeSH
• komprese míchy metabolismus   patologie   terapie   MeSH
• krysa rodu Rattus MeSH
• kultivované buňky MeSH
• luminescentní proteiny metabolismus   MeSH
• magnetická rezonanční tomografie metody   MeSH
• mícha cytologie   metabolismus   transplantace   ultrastruktura   MeSH
• mozek cytologie   metabolismus   ultrastruktura   MeSH
• myši MeSH
• neuroglie metabolismus   patologie   ultrastruktura   MeSH
• neurony metabolismus   patologie   ultrastruktura   MeSH
• poranění mozku metabolismus   patologie   terapie   MeSH
• potkani Wistar MeSH
• transplantace kmenových buněk metody   MeSH
• transplantace kostní dřeně metody   MeSH
• zelené fluorescenční proteiny MeSH
• železité sloučeniny metabolismus   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• Názvy látek
• ferric oxide MeSH Prohlížeč
• fosfopyruváthydratasa MeSH
• gliový fibrilární kyselý protein MeSH
• luminescentní proteiny MeSH
• zelené fluorescenční proteiny MeSH
• železité sloučeniny MeSH

Chondroblastomas are unusual cartilage benign lesions of bone that have well-characterized histological features. We reviewed and immunohistochemically examined the paraffin block material of 20 cases, and in seven tumors of this collection we found distinct cytoplasmic muscle-specific actin positivity of some tumor chondroblasts and chondrocytes. Muscle-specific actin-positive cells had the histological and ultrastructural features typical of chondroblasts. Moreover, in their cytoplasm they contained bundles of microfilaments with focal densities, as is typical of myofilaments. We did not observe any basal lamina around these cells, which were surrounded by intercellular matrix of the cartilage tissue type. Therefore, we suppose that muscle-specific actin-positive cells occurring in some chondroblastomas do not represent an admixture of myofibroblasts but chondroblasts with actin expression. The unusual immunophenotype of some chondroblasts might be the result of aberrant actin expression or of the plasticity of their phenotype modulated by microenvironmental stimuli. It is a question of whether, analogously to the terminology of myofibroblasts, such cells could be designated as myochondroblasts.

• MeSH
• aktiny metabolismus   MeSH
• chondroblastom metabolismus   patologie   ultrastruktura   MeSH
• chondrom metabolismus   MeSH
• chondrosarkom metabolismus   MeSH
• chrupavka metabolismus   patologie   ultrastruktura   MeSH
• elektronová mikroskopie MeSH
• fosfopyruváthydratasa metabolismus   MeSH
• imunohistochemie MeSH
• lidé MeSH
• proteiny S100 metabolismus   MeSH
• vimentin metabolismus   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• aktiny MeSH
• fosfopyruváthydratasa MeSH
• proteiny S100 MeSH
• vimentin MeSH

INTRODUCTION: Malignant granular cell tumor (MGCT) of the esophagus is an extremely rare malignancy with a poor prognosis. Literature describing this condition is not sufficient, especially regarding long-term survival. PRESENTATION OF CASE: A 52-year-old woman presented with dyspnea and slow onset dysphagia. The endoscopy, endoscopic ultrasound (EUS), bronchoscopy, and positron emission tomography (PET)/computed tomography (CT) supported the suspicion of esophageal gastrointestinal stromal tumor (GIST). Open wedge esophagectomy and tracheal resection were performed. The histology proved periodic acid-Schiff (PAS)-positive granules in epithelial cells, hyperchromatic nuclei and the positivity of Protein soluble in 100% ammonium sulfate (S-100), vimentin, neuron-specific enolase, laminin, and myelinic proteins. Local recurrence after 10 months required a two-phase esophagectomy with retrosternal gastroplasty. Bone, liver, and mediastinal metastases occurred 6 months later, with overall survival of 34 months. DISCUSSION: Preoperative histological confirmation is often not reliable. Tracheal invasion increases the perioperative risk and the probability of an unsuccessful resection. Esophagectomy or radical R0 local resection is the only known curative therapy. Repeated resections may increase survival in case of locoregional recurrence. Radiotherapy has a potential for palliative care. CONCLUSION: Esophageal MGCT requires a detailed presentation including long-term survival. Early surgical removal of intramural esophageal neoplasms with potentially malignant features is highly recommended. Radical and/or repeated esophageal resections are the only known therapies with curative potential.

• Klíčová slova
• esophageal neoplasm, esophagectomy, granular cell tumor, local case report, neoplasm recurrence,
• MeSH
• časové faktory MeSH
• ezofagektomie MeSH
• fatální výsledek MeSH
• lidé středního věku MeSH
• lidé MeSH
• lokální recidiva nádoru MeSH
• nádor z granulárních buněk diagnostické zobrazování   sekundární   chirurgie   MeSH
• nádory jícnu diagnostické zobrazování   patologie   chirurgie   MeSH
• progrese nemoci MeSH
• výsledek terapie MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH