We have searched for polymorphism of inducible nitric oxide synthase gene (Nos2 gene) in the Prague colony of salt-sensitive and salt-resistant Dahl/Rapp rats. Specific primers were used to confirm previously described Nos2 gene polymorphism because this gene was suggested to be a potential candidate gene for genetic hypertension. Phenotyping (blood pressure, organ weight, plasma lipids) have confirmed the data known from other colonies of Dahl/Rapp rats. However, in our colony we were not able to find any Nos2 gene polymorphism between salt-sensitive and salt-resistant rats, which was previously described in animals from Harlan colony. Moreover, the genetic homogeneity of our salt-sensitive and salt-resistant rats in terms of Nos2 gene was the same as in the original Brookhaven colony of Dahl rats. This is surprising because our colony has been established from breeding pairs kindly provided by Prof. J.P. Rapp more than 15 years ago. It seems that the polymorphism found in Harlan colony could be the result of previous contamination or genetic drift during the breeding conditions specific for this colony.

• MeSH
• krevní tlak genetika   MeSH
• krysa rodu Rattus MeSH
• polymorfismus genetický genetika   MeSH
• potkani inbrední Dahl genetika   MeSH
• synthasa oxidu dusnatého, typ II nedostatek   genetika   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• Geografické názvy
• Česká republika MeSH
• Názvy látek
• Nos2 protein, rat MeSH Prohlížeč
• synthasa oxidu dusnatého, typ II MeSH

Pulmonary arterial hypertension (PAH) isa fatal disease characterized by vascular remodeling and chronic inflammation. Macrophages are the key orchestrators of inflammatory and repair responses, and have been demonstrated to be vital in the pathogenesis of PAH. However, specific phenotype of macrophage polarization (M1 & M2 macrophage) in the development of PAH and the underlying mechanisms how they work are still largely unclear. A rat model of monocrotaline (MCT) induced PAH was used. Hemodynamic analysis and histopathological experiments were conducted at day 3, 7, 14, 21 and 28, respectively. In PAH rat lung tissue, confocal microscopic images showed that CD68+NOS2+ M1-like macrophages were remarkably infiltrated on early stage, but dramatically decreased in mid-late stage. Meanwhile, CD68+CD206+ M2-like macrophages in lung tissue accumulated gradually since day 7 to day 28, and the relative ratio of M2/M1 macrophage increased over time. Results detected by western blot and immunohistochemistry were consistent. Further vitro functional studies revealed the possible mechanism involved in this pathophysiological process. By using Transwell co-culture system, it was found that M1 macrophages inducedendothelial cellapoptosis, while M2 macrophages significantly promoted proliferation of both endothelial cell and smooth muscle cell.These data preliminarily demonstrated a temporal dynamic change of macrophage M1/M2 polarization status in the development of experimental PAH. M1 macrophages participated in the initial stage of inflammation by accelerating apoptosis of endothelial cell, while M2 macrophages predominated in the reparative stage of inflammation and the followed stage of aberrant tissue remodeling.

• MeSH
• antigeny diferenciační myelomonocytární metabolismus   MeSH
• apoptóza MeSH
• arteria pulmonalis metabolismus   patologie   MeSH
• časové faktory MeSH
• CD antigeny metabolismus   MeSH
• cytokiny metabolismus   MeSH
• endoteliální buňky pupečníkové žíly (lidské) metabolismus   patologie   MeSH
• fenotyp MeSH
• kokultivační techniky MeSH
• krysa rodu Rattus MeSH
• kultivované buňky MeSH
• lidé MeSH
• makrofágy metabolismus   patologie   MeSH
• mediátory zánětu metabolismus   MeSH
• modely nemocí na zvířatech MeSH
• monokrotalin MeSH
• myocyty hladké svaloviny metabolismus   patologie   MeSH
• plicní arteriální hypertenze chemicky indukované   metabolismus   patologie   MeSH
• potkani Sprague-Dawley MeSH
• proliferace buněk MeSH
• receptor mannózy metabolismus   MeSH
• remodelace cév * MeSH
• synthasa oxidu dusnatého, typ II metabolismus   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• lidé MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antigeny diferenciační myelomonocytární MeSH
• CD antigeny MeSH
• CD68 protein, rat MeSH Prohlížeč
• cytokiny MeSH
• mediátory zánětu MeSH
• monokrotalin MeSH
• Nos2 protein, rat MeSH Prohlížeč
• receptor mannózy MeSH
• synthasa oxidu dusnatého, typ II MeSH

Acyclic nucleoside phosphonates (ANP) are virostatics effective against viruses like hepatitis B virus and human immunodeficiency virus. Our previous reports indicated immunomodulatory activities of ANP in mouse and human innate immune cells. Recently, evidence has increased that hepatocytes may play an active role in immune regulation of the liver homeostasis or injury. In this study we investigated possible immunomodulatory effects of ANP on rat hepatocytes and macrophages. Nitric oxide (NO) production and secretion of cytokines (IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-10, IL-13, IL-18, IFN-γ, TNF-α and GM-CSF) were analyzed under in vitro conditions. Test compounds included: 9-[2-(phosphonomethoxy)ethyl]adenine (PMEA; adefovir); 9-[2-(phosphonomethoxy)ethyl]-2,6-diaminopurine (PMEDAP); (R)- and (S)-enantiomers of 9-[2-(phosphonomethoxy)propyl]adenine [(R)-PMPA; tenofovir] and [(S)-PMPA]; 9-[2-(phosphonomethoxy)propyl]-2,6-diaminopurine [(R)-PMPDAP] and [(S)-PMPDAP]. The group of test compounds also included their N(6)-substituted derivatives. Some of ANP which are able to induce NO production and cytokine secretion in cultured macrophages possess the same immunobiological activity in isolated hepatocytes. The extent of responses is in range of LPS/IFN-γ stimulation in both types of cells. The effects of active ANP on NO expression and cytokine secretion are dose- and time-dependent. Interestingly, the spectrum of detected cytokines induced by ANP is broader in hepatocytes. The results also confirm immunomodulatory effects of some ANP on rodent macrophages. Moreover, we demonstrate for the first time immunobiological reactivity of primary rat hepatocytes induced by exogenous ANP compounds. The potential of hepatocytes to synthesize cytokines can contribute to better understanding of liver immune function and can serve for pharmacological intervention in liver diseases.

• MeSH
• cytokiny imunologie   metabolismus   MeSH
• hepatocyty účinky léků   imunologie   metabolismus   MeSH
• krysa rodu Rattus MeSH
• lipopolysacharidy imunologie   farmakologie   MeSH
• makrofágy účinky léků   imunologie   metabolismus   MeSH
• nukleotidy cyklické imunologie   farmakologie   MeSH
• organofosfonáty imunologie   farmakologie   MeSH
• oxid dusnatý imunologie   metabolismus   MeSH
• potkani Wistar MeSH
• synthasa oxidu dusnatého, typ II imunologie   metabolismus   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• cytokiny MeSH
• lipopolysacharidy MeSH
• Nos2 protein, rat MeSH Prohlížeč
• nukleotidy cyklické MeSH
• organofosfonáty MeSH
• oxid dusnatý MeSH
• synthasa oxidu dusnatého, typ II MeSH

Corneal xenotransplantation may be an alternative approach to overcome shortage of allografts for clinical transplantation. Orthotopic corneal rat-to-mouse xenotransplantation and syngeneic transplantation was performed and the effects of anti-CD4 and anti-CD8 treatments on corneal xenograft survival and production of cytokines, interleukin (IL)-2, IL-4, IL-10, gamma-interferon (IFN-gamma) and nitric oxide (NO) were evaluated. RT-PCR was used to determine the expression of genes for cytokines and inducible nitric oxide synthase (iNOS) in the grafts. The presence of iNOS protein in grafts was detected by immunofluorescent staining. We found that corneal xenotransplantation was associated with a strong upregulation of genes for both Th1 and Th2 cytokines and with NO production in the graft. Treatment of xenograft recipients with mAb anti-CD4, but not anti-CD8, resulted in a profound inhibition of IL-2, IL-4 and IL-10 production, and in a significant prolongation of corneal xenograft survival. The results show that upregulation of Th2 cytokines after corneal xenotransplantation does not correlate with xenograft rejection. Rather, corneal graft rejection is associated with the expression of genes for IFN-gamma and iNOS and with NO production.

• MeSH
• antigeny CD4 imunologie   MeSH
• antigeny CD8 imunologie   MeSH
• cytokiny biosyntéza   MeSH
• exprese genu MeSH
• krysa rodu Rattus MeSH
• monoklonální protilátky farmakologie   MeSH
• myši inbrední BALB C MeSH
• myši MeSH
• oxid dusnatý biosyntéza   MeSH
• potkani inbrední LEW MeSH
• přežívání štěpu účinky léků   MeSH
• rohovka metabolismus   MeSH
• synthasa oxidu dusnatého, typ II MeSH
• synthasa oxidu dusnatého genetika   metabolismus   MeSH
• transplantace heterologní * MeSH
• transplantace rohovky * MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antigeny CD4 MeSH
• antigeny CD8 MeSH
• cytokiny MeSH
• monoklonální protilátky MeSH
• Nos2 protein, mouse MeSH Prohlížeč
• Nos2 protein, rat MeSH Prohlížeč
• oxid dusnatý MeSH
• synthasa oxidu dusnatého, typ II MeSH
• synthasa oxidu dusnatého MeSH

In the present study, we investigated whether erythropoietin (Epo) has a protective effect against cytotoxicity induced by interferon-gamma (IFN-gamma ) and lipopolysaccharide (LPS) in primary rat oligodendrocyte cultures. The possible modulatory effect of erythropoietin on inducible nitric oxide synthase (iNOS) mRNA expression and nitrite production were also analyzed. Erythropoietin exerted a significant protective effect against IFN-gamma and LPS-induced oligodendrocyte injury as determined by lactate dehydrogenase assay. Treatment with erythropoietin inhibited the expression of iNOS mRNA and nitrite production resulting from proinflammatory stimulation by IFN-gamma and LPS. These results suggest that erythropoietin has protective effects against inflammatory oligodendrocyte injury in vitro and may play a protective role in neurological disorders characterized by oligodendrocyte death, such as multiple sclerosis.

• MeSH
• erythropoetin farmakologie   MeSH
• interferon gama MeSH
• krysa rodu Rattus MeSH
• kultivované buňky MeSH
• lipopolysacharidy MeSH
• messenger RNA metabolismus   MeSH
• mozek MeSH
• neuroprotektivní látky farmakologie   MeSH
• novorozená zvířata MeSH
• oligodendroglie účinky léků   metabolismus   MeSH
• oxid dusnatý biosyntéza   metabolismus   MeSH
• potkani Wistar MeSH
• regulace genové exprese MeSH
• synthasa oxidu dusnatého, typ II biosyntéza   metabolismus   MeSH
• viabilita buněk MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• erythropoetin MeSH
• interferon gama MeSH
• lipopolysaccharide, E. coli O26-B6 MeSH Prohlížeč
• lipopolysacharidy MeSH
• messenger RNA MeSH
• neuroprotektivní látky MeSH
• Nos2 protein, rat MeSH Prohlížeč
• oxid dusnatý MeSH
• synthasa oxidu dusnatého, typ II MeSH

Valsartan has the potential to attenuate neointimal hyperplasia and to suppress the inflammatory response. This study aimed to evaluate the role of valsartan in neointimal hyperplasia and the toll-like receptor 4 (TLR4)-nitric oxide synthase (NOS) pathway in the balloon-injured rat aorta.Forty-eight Wistar rats were randomly allocated to three groups: sham control (control), balloon-injured group (surgery), and balloon-injured+valsartan-treated group (valsartan). Rats were killed at 14 and 28 days after balloon-injury, and then the aortic tissues were collected for morphometric analysis as well as for measurements of the mRNA or protein expression of angiotensin II, angiotensin II type 1 (AT1) receptor, angiotensin II type 2 (AT2) receptor, TLR4, endothelial nitric oxide synthase (eNOS), inducible NOS (iNOS), serine/arginine-rich splicing factor 1(SRSF1) and extracellular signal regulated kinase (ERK). Valsartan at a dose of 20 mg/kg/day markedly decreased neointimal hyperplasia in the aorta of balloon-injured rats, and significantly reduced the mRNA or protein expression of TLR4, AT1 receptor, SRSF1 and phosphorylated-ERK (p-ERK) as well as the aortic levels of iNOS (all p < 0.05). Moreover, valsartan increased the eNOS level and AT2 receptor mRNA and protein expression levels (all p < 0.05). Valsartan prevented neointimal hyperplasia and inhibited SRSF1 expression and the TLR4-iNOS-ERK-AT1 receptor pathway in the balloon-injured rat aorta.

• MeSH
• aorta účinky léků   enzymologie   patologie   MeSH
• blokátory receptoru 1 pro angiotenzin II farmakologie   MeSH
• extracelulárním signálem regulované MAP kinasy metabolismus   MeSH
• fosforylace MeSH
• hyperplazie MeSH
• krysa rodu Rattus MeSH
• modely nemocí na zvířatech MeSH
• nemoci aorty farmakoterapie   enzymologie   genetika   patologie   MeSH
• neointima * MeSH
• poranění cév farmakoterapie   enzymologie   genetika   patologie   MeSH
• potkani Wistar MeSH
• receptor angiotensinu typ 1 genetika   metabolismus   MeSH
• serin-arginin sestřihové faktory metabolismus   MeSH
• signální transdukce MeSH
• synthasa oxidu dusnatého, typ II metabolismus   MeSH
• toll-like receptor 4 genetika   metabolismus   MeSH
• valsartan farmakologie   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• blokátory receptoru 1 pro angiotenzin II MeSH
• extracelulárním signálem regulované MAP kinasy MeSH
• Nos2 protein, rat MeSH Prohlížeč
• receptor angiotensinu typ 1 MeSH
• serin-arginin sestřihové faktory MeSH
• SRSF1 protein, rat MeSH Prohlížeč
• synthasa oxidu dusnatého, typ II MeSH
• Tlr4 protein, rat MeSH Prohlížeč
• toll-like receptor 4 MeSH
• valsartan MeSH

Gastrodin, a main constituent of a Chinese herbal medicine, has been shown to be effective in treating various mood disorders. The purpose of the present study was to determine whether gastrodin could ameliorate stress-associated behavior in a rat model of enhanced single prolonged stress (ESPS)-induced posttraumatic stress disorder (PTSD). Following ESPS, rats were administered orally with gastrodin (50, 100, or 200 mg/kg daily) or vehicle for 2 weeks. Animals were then tested in the open field and elevated plus-maze, and the levels of IL-6 and IL-1beta, the expression of iNOS, p38 and phospho-p38 (p-p38) in hippocampus were also tested. ESPS exposure resulted in pronounced anxiety-like behavior, elevated IL-6 and IL-1beta levels, and the higher expression of iNOS and p-p38 in hippocampus. However, repeated treatment with gastrodin, particularly at higher doses, reversed the aforementioned changes, including anxiety-like behavior, levels of IL-6 and IL-1beta, and the expression of iNOS and the p38 MAPK phosphorylation. These results indicate that gastrodin possesses anxiolytic effect and may be an effective herbal preparation for the treatment of PTSD.

• MeSH
• anxiolytika aplikace a dávkování   farmakologie   MeSH
• aplikace orální MeSH
• benzylalkoholy aplikace a dávkování   farmakologie   MeSH
• časové faktory MeSH
• chování zvířat účinky léků   MeSH
• fosforylace MeSH
• glukosidy aplikace a dávkování   farmakologie   MeSH
• hipokampus účinky léků   enzymologie   MeSH
• interleukin-1beta metabolismus   MeSH
• interleukin-6 metabolismus   MeSH
• krysa rodu Rattus MeSH
• léky rostlinné čínské aplikace a dávkování   farmakologie   MeSH
• mitogenem aktivované proteinkinasy p38 metabolismus   MeSH
• modely nemocí na zvířatech MeSH
• pohybová aktivita účinky léků   MeSH
• posttraumatická stresová porucha farmakoterapie   enzymologie   psychologie   MeSH
• potkani Sprague-Dawley MeSH
• synthasa oxidu dusnatého, typ II metabolismus   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• anxiolytika MeSH
• benzylalkoholy MeSH
• gastrodin MeSH Prohlížeč
• glukosidy MeSH
• interleukin-1beta MeSH
• interleukin-6 MeSH
• léky rostlinné čínské MeSH
• mitogenem aktivované proteinkinasy p38 MeSH
• Nos2 protein, rat MeSH Prohlížeč
• synthasa oxidu dusnatého, typ II MeSH

The modulatory effect of FK 506 and cyclosporin A (CsA) on the expression of inducible nitric oxide synthase (iNOS) in macrophages and mechanisms of their action were analysed. Isolated rat peritoneal macrophages were cultured for 12 or 24 h with or without lipopolysaccharide (LPS) (5 microg/ml) and in the absence or presence of FK 506 or CsA (0.1 and 1 microg/ml). Total RNA from macrophages was isolated and the expression of the gene for iNOS was assessed by using RT-PCR. The concentration of NO2- in culture supernatants was taken as a measure of nitric oxide (NO) production. FK 506 (0.1 and 1 microg/ml) reduced the LPS-induced increase of NO2- levels by 68% and 81%, respectively. CsA (0.1 and 1 microg/ml) decreased levels of nitrites by 39% and 69%, respectively. The results obtained suggest that both immunosuppressive drugs exhibit dose-dependent inhibitory effect on NO production and that FK 506 is more potent agent than CsA, in this respect. FK 506 exhibits its inhibitory effect on a phosphatase at the transcriptional level in macrophages. iNOS expression down-regulation by CsA is occurred post-transcriptionally.

• MeSH
• cyklosporin farmakologie   MeSH
• down regulace MeSH
• elektroforéza v agarovém gelu MeSH
• imunosupresiva farmakologie   MeSH
• krysa rodu Rattus MeSH
• kultivované buňky MeSH
• lipopolysacharidy farmakologie   MeSH
• messenger RNA metabolismus   MeSH
• oxid dusnatý metabolismus   MeSH
• peritoneální makrofágy účinky léků   enzymologie   MeSH
• polymerázová řetězová reakce s reverzní transkripcí MeSH
• potkani Wistar MeSH
• regulace genové exprese enzymů MeSH
• synthasa oxidu dusnatého, typ II MeSH
• synthasa oxidu dusnatého genetika   metabolismus   MeSH
• takrolimus farmakologie   MeSH
• vztah mezi dávkou a účinkem léčiva MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• cyklosporin MeSH
• imunosupresiva MeSH
• lipopolysacharidy MeSH
• messenger RNA MeSH
• Nos2 protein, rat MeSH Prohlížeč
• oxid dusnatý MeSH
• synthasa oxidu dusnatého, typ II MeSH
• synthasa oxidu dusnatého MeSH
• takrolimus MeSH

Certain liver metabolic diseases point to the presence of disturbances in glycogen deposition. Epinephrine raises the cAMP level that activates protein kinase A leading to the activation of phosphorylase and glycogen breakdown. In the present report, we sought to investigate whether NO is produced during adrenoceptor agonist-induced glycogenolysis in rat hepatocytes in cultures. Isolated glycogen rich rat hepatocytes in cultures were used. NO production (NO(2)(-)) was assessed under the effect of adrenergic agonists and adrenergic agonist/antagonist pairs, dibutyryl cyclic AMP sodium-potassium salt (db-cAMP), NO synthase (NOS) inhibitors N(omega)-nitro-L-arginine methyl ester (L-NAME), aminoguanidine (AG) and the NO donor S-nitroso-N-acetyl penicillamine (SNAP). The inducible NO synthase (iNOS) mRNA was examined by the reverse transcription-polymerase chain reaction (RT-PCR). Glycogenolysis was quantified by glucose levels released into medium. The amount of glucose and NO(2)(-) released by hepatocytes was increased as a result of epinephrine, phenylephrine or db-cAMP treatments. The increase in glucose and NO(2)(-) released by epinephrine or phenylephrine was blocked or reduced by prazosin pretreatment and by NOS inhibitors aminoguanidine and L-NAME. iNOS gene expression was up-regulated by epinephrine. It can be concluded that glycogenolysis occurs through -adrenoceptor stimulation and a signaling cascade may involve NO production.

• MeSH
• adrenalin metabolismus   MeSH
• adrenergní receptory účinky léků   metabolismus   MeSH
• agonisté adrenergních alfa-receptorů farmakologie   MeSH
• alfa blokátory farmakologie   MeSH
• časové faktory MeSH
• CMP cyklický analogy a deriváty   farmakologie   MeSH
• donory oxidu dusnatého farmakologie   MeSH
• enzymová indukce MeSH
• fenylefrin farmakologie   MeSH
• glukosa metabolismus   MeSH
• glykogenolýza účinky léků   MeSH
• guanidiny farmakologie   MeSH
• hepatocyty účinky léků   enzymologie   metabolismus   MeSH
• inhibitory enzymů farmakologie   MeSH
• krysa rodu Rattus MeSH
• kultivované buňky MeSH
• NG-nitroargininmethylester farmakologie   MeSH
• oxid dusnatý metabolismus   MeSH
• potkani Wistar MeSH
• prazosin farmakologie   MeSH
• S-nitroso-N-acetylpenicilamin farmakologie   MeSH
• synthasa oxidu dusnatého, typ II antagonisté a inhibitory   biosyntéza   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• adrenalin MeSH
• adrenergní receptory MeSH
• agonisté adrenergních alfa-receptorů MeSH
• alfa blokátory MeSH
• CMP cyklický MeSH
• dibutyryl cyclic-3',5'-cytidine monophosphate MeSH Prohlížeč
• donory oxidu dusnatého MeSH
• fenylefrin MeSH
• glukosa MeSH
• guanidiny MeSH
• inhibitory enzymů MeSH
• NG-nitroargininmethylester MeSH
• Nos2 protein, rat MeSH Prohlížeč
• oxid dusnatý MeSH
• pimagedine MeSH Prohlížeč
• prazosin MeSH
• S-nitroso-N-acetylpenicilamin MeSH
• synthasa oxidu dusnatého, typ II MeSH

Although exposure to continuous light is associated with hypertension and modulates the outcome of ischemia-reperfusion injury, less attention has been paid to its effects on cardiac morphology. We investigated whether 4-week exposure of experimental rats to continuous 24 h/day light can modify cardiac morphology, with focus on heart weight, fibrosis and collagen I/III ratio in correlation with NO-synthase expression. Two groups of male adult Wistar rats were studied: controls exposed to normal light/dark cycle (12 h/day light, 12 h/day dark) and rats exposed to continuous light. After 4 weeks of treatment the absolute and the relative heart weights were determined and myocardial fibrosis and collagen type I/III ratio were evaluated using picrosirius red staining. Endothelial and inducible NO-synthase expression was detected immunohistochemically. The exposure of rats to continuous light resulted in an increase of body weight with proportionally increased heart weight. Myocardial fibrosis remained unaffected but collagen I/III ratio increased. Neither endothelial nor inducible NO-synthase expression was altered in light-exposed rats. We conclude that the loss of structural homogeneity of the myocardium in favor of collagen type I might increase myocardial stiffness and contribute to functional alterations after continuous light exposure.

• MeSH
• časové faktory MeSH
• fibróza MeSH
• kardiomyocyty enzymologie   patologie   účinky záření   MeSH
• kolagen typ III metabolismus   MeSH
• kolagen typu I metabolismus   MeSH
• krysa rodu Rattus MeSH
• potkani Wistar MeSH
• světlo * MeSH
• synthasa oxidu dusnatého, typ II metabolismus   MeSH
• synthasa oxidu dusnatého, typ III MeSH
• tělesná hmotnost účinky záření   MeSH
• velikost orgánu účinky záření   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• kolagen typ III MeSH
• kolagen typu I MeSH
• Nos2 protein, rat MeSH Prohlížeč
• Nos3 protein, rat MeSH Prohlížeč
• synthasa oxidu dusnatého, typ II MeSH
• synthasa oxidu dusnatého, typ III MeSH