The association of silver nanoparticles (AgNps) to sealant agent Palaseal® can be a promising alternative for complete denture wearers who may develop denture stomatitis (DS). The study aimed to evaluate the anti-Candida and biocompatible potential of silver nanoparticles synthesized by three routes associated with denture glaze to prevent and/or treat oral candidiasis. Surface acrylic resin specimens were treated with different associations of glaze with AgNps (VER+AgUV, VER+AgTurk and VER+AgGm). As controls, specimens were treated with glaze+nystatin (VER+Nyst), glaze only (VER) or submerged in PBS (PBS). Afterwards, Candida albicans biofilm was developed for 24 h, 15 d and 30 d. Subsequently, the biofilm was quantified by CFU/mL, XTT assay and confocal laser scanning microscopy. Fibroblasts were submitted to conditioned medium with the same associations for 24, 48 and 72 h and LIVE/DEAD® viability test was carried out. Regardless of the period, there was a significant reduction (p < 0.01) of viable fungal cells load, as well as inhibition of fungal metabolic activity, in specimens treated with glaze+AgNps associations, compared to VER and PBS. The anti-Candida effects of the associations were similar to the VER+Nyst group, with emphasis on VER+AgGm, which showed the highest percentage values of non-viable fungal cells maintained over time. The associations did not prove toxicity to fibroblasts. The AgNps exerted antimicrobial activity against C. albicans biofilms and are biocompatible. The most effective results were achieved with the association of glaze+silver nanoparticles synthesized by the green chemistry method (AgGm), proving to be an innovative alternative in the management of DS.

• Klíčová slova
• Candida albicans, Silver nanoparticles, Stomatitis under denture, Surface sealant agent,
• MeSH
• antifungální látky * farmakologie   chemie   MeSH
• biofilmy * účinky léků   MeSH
• Candida albicans * účinky léků   MeSH
• fibroblasty účinky léků   MeSH
• kovové nanočástice * chemie   MeSH
• lidé MeSH
• mikrobiální testy citlivosti MeSH
• orální kandidóza farmakoterapie   mikrobiologie   MeSH
• stomatitida vyvolaná protézou * mikrobiologie   farmakoterapie   MeSH
• stříbro * farmakologie   chemie   MeSH
• zubní náhrady mikrobiologie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antifungální látky * MeSH
• stříbro * MeSH

This study investigated the effect of peroxide bleaching gel on the durability of the adhesive bond between composite material, enamel, and dentin created with the etch-and-rinse adhesive Gluma Comfort Bond (GLU) and with the self-etch adhesives Clearfil SE Bond (CLE), Adper Prompt (ADP), and iBond (IBO). The adhesives were applied to flattened enamel and dentin of extracted human molars and built up with a microhybrid composite (Charisma). After 25 eight-hour cycles of bleaching with a 20% carbamide peroxide bleaching gel (Opalescence PF 20), the shear bond strength was measured and compared with one-day and two-month control specimens stored in water. The data were analyzed using nonparametric Mann-Whitney and Kruskal-Wallis statistics (p<0.05). Detailed fractographic analysis was performed using scanning electron microscopy. The bleaching gel significantly decreased the bond strength on both enamel and dentin for the simplified single-step self-etch adhesives ADP and IBO and markedly affected a fracture pattern of ADP specimens at the periphery of their bonded area. The results of our study indicate that the durability of adhesive restorations can be detrimentally influenced by carbamide peroxide bleaching and that different adhesives show varying sensitivity levels to the bleaching gel.

• MeSH
• bělení zubů metody   MeSH
• bisfenol A-glycidyl methakrylát chemie   MeSH
• časové faktory MeSH
• dentin ultrastruktura   MeSH
• dentinová adheziva chemie   MeSH
• karbamidperoxid MeSH
• látky na bělení zubů chemie   MeSH
• leptání zubů kyselinou metody   MeSH
• lidé MeSH
• mechanický stres MeSH
• mikroskopie elektronová rastrovací MeSH
• močovina analogy a deriváty   chemie   MeSH
• organofosfáty chemie   MeSH
• peroxidy chemie   MeSH
• pevnost ve smyku MeSH
• povrchové vlastnosti MeSH
• pryskyřičné cementy chemie   MeSH
• složené pryskyřice chemie   MeSH
• testování materiálů MeSH
• vazba zubní * MeSH
• voda chemie   MeSH
• zubní materiály chemie   MeSH
• zubní sklovina ultrastruktura   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• randomizované kontrolované studie MeSH
• srovnávací studie MeSH
• Názvy látek
• Adper Prompt Self-Etch MeSH Prohlížeč
• bisfenol A-glycidyl methakrylát MeSH
• Charisma composite resin MeSH Prohlížeč
• Clearfil SE Bond MeSH Prohlížeč
• dentinová adheziva MeSH
• Gluma Comfort Bond MeSH Prohlížeč
• iBond MeSH Prohlížeč
• karbamidperoxid MeSH
• látky na bělení zubů MeSH
• močovina MeSH
• organofosfáty MeSH
• peroxidy MeSH
• pryskyřičné cementy MeSH
• složené pryskyřice MeSH
• voda MeSH
• zubní materiály MeSH

PURPOSE: To estimate the in vitro reliability of typical self-etching and etch-and-rinse adhesives of various application protocols. MATERIALS AND METHODS: The following adhesives were applied on flat dentin surfaces of extracted human teeth (n = 223): self-etching two-step adhesives: AdheSE (AH), Clearfil SE Bond (CL), OptiBond SE (OS); one-step adhesives: Adper Prompt L-Pop (ADP), Adper Prompt (AD), and Xeno III (XE); all-in-one adhesive: iBond (IB); etch-and-rinse three-step adhesives: OptiBond FL (OF), two-step Gluma Comfort Bond (G), Excite (E) and Prime & Bond NT (PB). Composite buildups were prepared using a microhybrid composite, Opticor New. Shear bond strength was determined after 24 h of storage at 37 degrees C in distilled water. The results were analyzed with a nested ANOVA (adhesive, type of adhesive) followed by the Fisher post-hoc tests of group homogeneity at alpha = 0.05. A two-parameter Weibull distribution was used to calculate the critical shear bond strength corresponding to 5% probability of failure as a measure of system reliability. RESULTS: ANOVA revealed a significant decrease (p < 0.001) in the mean shear bond strength as follows: AH=CL=OS=G=E=OF>AD=IB=XE>PB=ADP, but no significant difference (p > 0.48) between the etch-and-rinse and self-etching adhesives. The corresponding characteristic bond strength of Weibull distribution ranged between 24.1 and 12.1 MPa, Weibull modulus between 8.3 and 2.1, and the critical shear bond strength varied from 16.0 to 3.0 MPa. CONCLUSION: Pronounced differences in the critical shear bond strength suggest reliability variations in the adhesive systems tested, which originate from chemical composition rather than type of adhesive.

• MeSH
• akrylové pryskyřice chemie   MeSH
• bisfenol A-glycidyl methakrylát chemie   MeSH
• časové faktory MeSH
• dentin ultrastruktura   MeSH
• dentinová adheziva chemie   MeSH
• kyseliny polymethakrylové chemie   MeSH
• leptání zubů kyselinou metody   MeSH
• lidé MeSH
• mechanický stres MeSH
• methakryláty chemie   MeSH
• organofosfáty chemie   MeSH
• pevnost ve smyku MeSH
• pravděpodobnost MeSH
• pryskyřičné cementy chemie   MeSH
• složené pryskyřice chemie   MeSH
• teplota MeSH
• testování materiálů MeSH
• vazba zubní * MeSH
• voda chemie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• validační studie MeSH
• Názvy látek
• AdheSE cement MeSH Prohlížeč
• Adper Prompt Self-Etch MeSH Prohlížeč
• akrylové pryskyřice MeSH
• bisfenol A-glycidyl methakrylát MeSH
• Clearfil SE Bond MeSH Prohlížeč
• dentinová adheziva MeSH
• Excite cement MeSH Prohlížeč
• Gluma Comfort Bond MeSH Prohlížeč
• iBond MeSH Prohlížeč
• kyseliny polymethakrylové MeSH
• methakryláty MeSH
• OptiBond FL MeSH Prohlížeč
• OptiBond Solo Plus Self-Etch MeSH Prohlížeč
• organofosfáty MeSH
• Prime and Bond NT MeSH Prohlížeč
• Prompt L-Pop MeSH Prohlížeč
• pryskyřičné cementy MeSH
• složené pryskyřice MeSH
• voda MeSH
• Xeno III MeSH Prohlížeč