To gain a better understanding of the progression of progenitor cells in the odontoblast lineage, we have examined and characterized the expression of a series of GFP reporters during odontoblast differentiation. However, previously reported GFP reporters (pOBCol2.3-GFP, pOBCol3.6-GFP, and DMP1-GFP), similar to the endogenous proteins, are also expressed by bone-forming cells, which made it difficult to delineate the two cell types in various in vivo and in vitro studies. To overcome these difficulties we generated DSPP-Cerulean/DMP1-Cherry transgenic mice using a bacterial recombination strategy with the mouse BAC clone RP24-258g7. We have analyzed the temporal and spatial expression of both transgenes in tooth and bone in vivo and in vitro. This transgenic animal enabled us to visualize the interactions between odontoblasts and surrounding tissues including dental pulp, ameloblasts and cementoblasts. Our studies showed that DMP1-Cherry, similar to Dmp1, was expressed in functional and fully differentiated odontoblasts as well as osteoblasts, osteocytes and cementoblasts. Expression of DSPP-Cerulean transgene was limited to functional and fully differentiated odontoblasts and correlated with the expression of Dspp. This transgenic animal can help in the identification and isolation of odontoblasts at later stages of differentiation and help in better understanding of developmental disorders in dentin and odontoblasts.

• Klíčová slova
• Odontoblasts, bone, dentin matrix protein 1, dentin sialophosphoprotein, fluorescent protein reporters,
• MeSH
• buněčná diferenciace MeSH
• extracelulární matrix - proteiny genetika   MeSH
• fluorescenční barviva MeSH
• fosfoproteiny genetika   MeSH
• myši transgenní MeSH
• myši MeSH
• odontoblasty cytologie   MeSH
• reportérové geny * MeSH
• sialoglykoproteiny genetika   MeSH
• transgeny MeSH
• zelené fluorescenční proteiny genetika   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Research Support, N.I.H., Extramural MeSH
• Názvy látek
• dentin sialophosphoprotein MeSH Prohlížeč
• Dmp1 protein, mouse MeSH Prohlížeč
• extracelulární matrix - proteiny MeSH
• fluorescenční barviva MeSH
• fosfoproteiny MeSH
• sialoglykoproteiny MeSH
• zelené fluorescenční proteiny MeSH

The organic matrix of the developing enamel is highly heterogeneous, comprising proteins derived from a number of different genes, including amelogenin, enamelin, ameloblastin, tuftelin, dentine sialophosphoprotein, enzymes and serum proteins. Each of these classes appears to undergo post-secretory sequential degradation which contributes towards further matrix heterogeneity. Possible functions of these proteins are discussed. Proteins of the organic matrix are presumed to play an important role in the modulation of mineral deposition and growth during tooth morphogenesis but the precise functions of individual matrix proteins remain unclear. (Tab. 1, Fig. 2, Ref. 47).

• MeSH
• lidé MeSH
• molekulární sekvence - údaje MeSH
• proteiny zubní skloviny chemie   fyziologie   MeSH
• sekvence aminokyselin MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH
• Názvy látek
• proteiny zubní skloviny MeSH