Disseminated fusariosis is a life-threatening, invasive, opportunistic infection in immunocompromised patients, especially those with haematological malignancies. The prognosis is poor because these fungi are resistant to many of the available antifungal agents. We present a case of disseminated fusariosis caused by Fusarium proliferatum in a patient with severe aplastic anaemia complicated by a secondary infection of Aspergillus flavus, with a fatal outcome. We also review the documented Fusarium infections in immunocompromised hosts.

• Klíčová slova
• Fusarium proliferatum, PCR diagnostics, fusariosis, real-time PCR,
• MeSH
• antifungální látky farmakologie   terapeutické užití   MeSH
• aplastická anemie komplikace   MeSH
• Aspergillus flavus účinky léků   izolace a purifikace   MeSH
• aspergilóza komplikace   mikrobiologie   MeSH
• fatální výsledek MeSH
• fusarióza komplikace   diagnóza   farmakoterapie   mikrobiologie   MeSH
• Fusarium účinky léků   izolace a purifikace   MeSH
• hostitel s imunodeficiencí * MeSH
• koinfekce MeSH
• lidé MeSH
• mladý dospělý MeSH
• oportunní infekce diagnóza   farmakoterapie   mikrobiologie   MeSH
• triazoly terapeutické užití   MeSH
• Check Tag
• lidé MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antifungální látky MeSH
• posaconazole MeSH Prohlížeč
• triazoly MeSH

The effect of volatile and gaseous metabolites released by germinating seeds of lentil cultivars more and less susceptible to fusariosis on the germination of spores of Mucor racemosus, Trichoderma viride, Verticillium dahliae and Botrytis cinerea was found to depend rather on the fungal genus than on the lentil cultivar. However, spores of Fusarium oxysporum reacted more sensitively during germination to the presence of exudates of both cultivars, when the more susceptible lentil displayed a stimulation, the less susceptible one an inhibition of spore germination. The greatest difference in the effect of exudates was observed in the more and less susceptible maize cultivars with respect to the germination of chlamydospores of Ustilago maydis, especially during the first hours of seed germination. Analysis of the exudates of germinating seeds showed the release of a greater amount of ethanol and methanol with acetaldehyde by the more susceptible cultivars of lentil and particularly maize.

• MeSH
• ethanol metabolismus   farmakologie   MeSH
• Fabaceae metabolismus   MeSH
• Fusarium patogenita   MeSH
• kukuřice setá metabolismus   MeSH
• léčivé rostliny * MeSH
• methanol metabolismus   farmakologie   MeSH
• mitosporické houby fyziologie   MeSH
• Mucor fyziologie   MeSH
• mykózy etiologie   MeSH
• nemoci rostlin MeSH
• půdní mikrobiologie * MeSH
• semena rostlinná metabolismus   MeSH
• spory hub fyziologie   MeSH
• Trichoderma fyziologie   MeSH
• volatilizace MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH
• Názvy látek
• ethanol MeSH
• methanol MeSH

Fusarium head blight (FHB) disease adversely affects grain quality and final yield in small-grain cereals including barley. In the present study, the effect of an artificial infection with Fusarium culmorum and an application of deoxynivalenol (DON) on barley spikes of cultivars Chevron and Pedant during flowering was investigated at grain mid-dough stage (BBCH 73) 10days after pathogen inoculation (10 dai). Proteomic analysis using a two-dimensional differential gel electrophoresis (2D-DIGE) technique coupled with LC-MS/MS investigated 98 protein spots revealing quantitative or qualitative differences between the experimental variants. Protein functional annotation of 93 identified protein spots revealed that most affected functional groups represent storage proteins (globulins, hordeins), followed by proteins involved in carbohydrate metabolism (α-amylase inhibitor, β-amylase, glycolytic enzymes), amino acid metabolism (aminotransferases), defence response (chitinase, xylanase inhibitor, serpins, SGT1, universal stress protein USP), protein folding (chaperones, chaperonins), redox metabolism (ascorbate-glutathione cycle), and proteasome-dependent protein degradation. The obtained results indicate adverse effects of infection on plant proteome as well as an active plant response to pathogen as shown by enhanced levels of several inhibitors of pathogen-produced degradation enzymes (α-amylase inhibitor, xylanase inhibitor, serpins), chaperones, and other stress-related proteins (SGT1, USP). Genotypic differences were found in hordein abundance between Chevron and Pedant.

• Klíčová slova
• Barley, Deoxynivalenol, Fusarium culmorum, Fusarium head blight, Storage proteins, Stress-related proteins,
• MeSH
• druhová specificita MeSH
• fusarióza * MeSH
• Fusarium patogenita   MeSH
• ječmen (rod) chemie   účinky léků   mikrobiologie   MeSH
• proteom účinky léků   MeSH
• proteomika metody   MeSH
• trichotheceny farmakologie   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• deoxynivalenol MeSH Prohlížeč
• proteom MeSH
• trichotheceny MeSH

INTRODUCTION: Fusarium species are common soil saprophytes and plant pathogens. Members of the genus have been frequently reported as etiologic agents of opportunistic infections in humans and animals. We report six cases of confirmed or suspected onychomycosis caused by members of the genus Fusarium (F. solani and F. oxysporum species complexes). MATERIAL AND METHODS: The isolates were identified by rDNA ITS sequencing analysis. The EMBL accession numbers for the ITS are HE974453-HE974458. A disk diffusion method was used for in vitro susceptibility testing. Comparison of disks (ITEST) and Neo-Sensitabs tablets (Rosco) on a different media at two different temperatures (25 °C and 35 °C) was made. RESULTS: Six strains of Fusarium spp. (4 strains of F. solani and 2 strains of F. oxysporum) were isolated from patients with confirmed or suspected onychomycosis. Natamycin (pimaricin) was the only antifungal effective in vitro in all isolates tested. Variable susceptibility of the isolates was detected in amphotericin B, econazole and terbinafine. The remaining antifungals tested were not effective. The results varied depending on the culture medium and temperature for nystatin and econazole disks and amphotericin B and terbinafin tablets. CONCLUSION: It is important to adhere to recommended methods when testing in vitro susceptibility to antifungals in moulds. An incubation temperature of 35 °C is important for obtaining valid results in amphotericin B tablets (and probably also terbinafine ones). Determination of multidrug-resistant Fusarium spp. in onychomycosis make the choice of therapy difficult. Good clinical effect was recorded with nail plate ablation and subsequent local econazole therapy.

• MeSH
• antifungální látky terapeutické užití   MeSH
• dospělí MeSH
• fungální léková rezistence * MeSH
• fusarióza farmakoterapie   MeSH
• lidé MeSH
• mikrobiální testy citlivosti MeSH
• onychomykóza farmakoterapie   mikrobiologie   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• kazuistiky MeSH
• Názvy látek
• antifungální látky MeSH

The submitted review deals with the problem of hyalohyphomycoses caused by the genus Fusarium. Attention is drawn to the fact that members of this genus cannot be considered any longer in medical microbiology as mere saprophytes but that they are able to cause superficial, organ and disseminated infections. The number of patients infected with these fungi is increasing.

• MeSH
• Fusarium * MeSH
• lidé MeSH
• mykózy * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• přehledy MeSH

Nowadays, free flap reconstruction in devastating lower limb trauma is a standard procedure in reconstructive surgery. The greatest factor directly affecting limb salvage is still the risk of infectious complications, whether local or systemic. Fungal wound infections are not among the most common infection complications in surgery, but their low incidence is compensated for by their fulminant and serious course, as well as severe local tissue destruction and strong angio-invasive potential together with the possibility of dissemination. In this case study, we present an example of a devastating lower leg injury, solved using latissimus free flap reconstruction, with subsequent difficult and prolonged healing, due to an invasive filamentous fungi infection. In the final part of the article, we focus briefly on the occurrence of similar cases in the literature.

• Klíčová slova
• filamentous fungi, free flap, infection, reconstruction,
• MeSH
• fusarióza * MeSH
• lidé MeSH
• Mucor MeSH
• poranění nohy (od hlezna dolů) * chirurgie   MeSH
• retrospektivní studie MeSH
• volné tkáňové laloky * MeSH
• výsledek terapie MeSH
• zákroky plastické chirurgie * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

Outbreak of exogenous Fusarium endophthalmitis after cataract surgery was evaluated. Twenty patients developed postoperative endophthalmitis. In 19 eyes, pars plana vitrectomy (PPV) was performed, in 14 cases (74 %) with primary intraocular lens explantation. In one case, the PPV was not performed because of poor general condition of the patient. Symptoms of endophthalmitis (damaged vision, iritis, tyndallization in anterior chamber, hypopyon) occurred at intervals of 16-79 days (mean 31.3 days). Fungal etiology was documented in 12 eyes (60 %). Fusarium oxysporum was evidenced by culture and/or microscopy and confirmed by PCR and sequencing analysis. Eighteen (90 %) patients were treated with oral voriconazole (400 mg/day) for a period of 4-6 weeks. The final visual acuity was 6/15 in 1 case (5 %), 6/60 and worse in 17 eyes (85 %), and in 2 cases (10 %), enucleation had to be performed. Viscoelastic filling material was suggested the most likely source of infection. Endophthalmitis caused by Fusarium spp. are a potentially big threat for patients with serious impact on vision. Successful management of the infection is highly dependent on early diagnosis including species identification and antifungal susceptibility testing, and on aggressive and long-term treatment.

• MeSH
• antifungální látky terapeutické užití   MeSH
• endoftalmitida farmakoterapie   epidemiologie   mikrobiologie   MeSH
• epidemický výskyt choroby MeSH
• extrakce katarakty škodlivé účinky   MeSH
• fusarióza farmakoterapie   epidemiologie   mikrobiologie   MeSH
• Fusarium patogenita   MeSH
• lidé středního věku MeSH
• lidé MeSH
• oční infekce mykotické farmakoterapie   MeSH
• pooperační komplikace mikrobiologie   MeSH
• pyrimidiny terapeutické užití   MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• triazoly terapeutické užití   MeSH
• vitrektomie MeSH
• vorikonazol MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antifungální látky MeSH
• pyrimidiny MeSH
• triazoly MeSH
• vorikonazol MeSH

OBJECTIVES: Emerging invasive fungal infections (IFI) have become a notable challenge. Apart from the more frequently described fusariosis, lomentosporiosis, mucormycosis, scedosporiosis, and certain dematiaceae or yeasts, little is known about extremely rare IFI. METHODS: Extremely rare IFI collected in the FungiScopeⓇ registry were grouped as Dematiaceae, Hypocreales, Saccharomycetales, Eurotiales, Dermatomycetes, Agaricales, and Mucorales. RESULTS: Between 2003 and June 2019, 186 extremely rare IFI were documented in FungiScopeⓇ. Dematiaceae (35.5%), Hypocreales (23.1%), Mucorales (11.8%), and Saccharomycetales (11.3%) caused most IFI. Most patients had an underlying malignancy (38.7%) with acute leukemia accounting for 50% of cancers. Dissemination was observed in 26.9% of the patients. Complete or partial clinical response rate was 68.3%, being highest in Eurotiales (82.4%) and in Agaricales (80.0%). Overall mortality rate was 29.3%, ranging from 11.8% in Eurotiales to 50.0% in Mucorales. CONCLUSIONS: Physicians are confronted with a complex variety of fungal pathogens, for which treatment recommendations are lacking and successful outcome might be incidental. Through an international consortium of physicians and scientists, these cases of extremely rare IFI can be collected to further investigate their epidemiology and eventually identify effective treatment regimens.

• Klíčová slova
• Invasive fungal infections, Mold, Rare, Registry, Yeast,
• MeSH
• antifungální látky terapeutické užití   MeSH
• invazivní mykotické infekce * farmakoterapie   epidemiologie   MeSH
• lidé MeSH
• mykózy * farmakoterapie   epidemiologie   MeSH
• registrace MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antifungální látky MeSH