Cieľ: Zistiť účinok zmesi aminokyseliny arginínu a dvojkombinácie antiglaukomatík Trusoptu so Xalatanom na fyziologické hodnoty vnútroočného tlaku (VOT) králikov. Metódy: Piatim dospelým králikom (samice plemena Novozélandský biely ) bol instilovaný 2% Trusopt (Dorzolamidi hydrochlorici) s 0.005% Xalatanom (Latanoprostum) a v týždňovom odstupe zmes 10% L-arginín.HCl v 2% trusopte so 0,005% xalatanom. VOT bol hodnotený v 0., 15., 30., 60., 120., 180., 240. minúte a 24. hodine po instilácii. Pravé oko toho istého jedinca bolo kontrolné. Výsledky: Kombinácia oboch antiglaukomatík už v 15. minúte signifikantne znížila VOT králikov oproti kontrolnému oku. Najväčší pokles VOT 6,5 torr bol v 240. minúte. V 24. hodine ich účinok sa ukázal ako nesignifikantný. Po instilácii zmesi L-arginínu.HCl v Trusopte so Xalatanom bol zaznamenaný počas 24 hodín signifikantný bifazický pokles VOT. Najväčší účinok tejto zmesi bol v 30. minúte (pokles o 5,1 torr). Jej preukazný účinok sa prejavil aj v 24. hodine (pokles o 2,5 torr). Hladina kontrolného oka v oboch pokusoch počas 24 hodín sa nemenila a bola na úrovni začiatočnej. Záver: V kombinácii dvoch antiglaukomatík Trusoptu so Xalatanom dané látky nepôsobia ako samostatné komponenty, ani aditívne, ale ako nová látka. Táto nová látka za in vivo podmienok v tkanivových štruktúrach oka slabo interaguje s voľnými amínokyselinami v spojovkovom vaku. V zmesi amínokyseliny L-arginínu v kombinácii dvoch antiglaukomatík (Trusoptu so Xalatanom) po interakcii in vitro vzniká nová biologicky aktívna látka, ktorá má na VOT mierne silnejší účinok.

To evaluate the effect of instilled amino acid L-arginine.HCl and combination of two antiglaucomatics (Trusopt with Xalatane mixture) on the physiological intraocular pressure IOP in rabbits. METHODS: Into the left conjunctival sac of 5 female rabbits of the New Zealand White species was instilled the mixture of 2% Trusopt (Dorsolamidi hydrochlorici) with 0.005% Xalatane (Latano-prostum) and after the one week break, the 10% L-arginine.HCl in 2% Trusopt with 0.005% Xalatane mixture. The IOPs were measured in 0, 5, 15, 30, 60, 120, 180, 240 min. and 24 hours after the instillation. The right eyes were used as controls. RESULTS: 1. The combination of two antiglaucomatics in comparison with the control eye decreased significantly the IOP value already after 15 min. The major decrease of the IOP (6.5 mm Hg) was observed after 240 min. After 24 hours, the effectiveness was not significant. 2. After instillation of the 10% L-arginine.HCl in 2% Trusopt with 0.005% Xalatane mixture, during the next 24 hours, the biphasic decrease of the IOP was established. The maximum of the effectiveness of this mixture is after 30 min (decrease by 5.1 mm Hg). The evidence of the effect was present also after 24 hour (decrease by 2.5 mm Hg). The mean value of the IOP of the control eyes in both groups of experiments in course of 24 hours was stable and was at the initial values. CONCLUSIONS: The combination of two antiglaucomatics (Trusopt with Xalatane mixture) was used. The effect of those two substances is not separate or additive but acts as a newly formed substance. In the structures of the eye, this new substance has only poor interaction with the free amino acids of the conjunctival sac in vivo. After in vitro interaction of the L-arginine.HCl and combination of two antiglaucomatics (Trusopt with Xalatane mixture), a new bioactive substance with stronger effect on physiologic IOP values was produced.

Klinika oftalmológie LF Univ Komenského Bratislava

Intraocular pressure after instillation of amino acid arginine and combination of two antiglaucomatics (Trusopt + Xalatane mixture) in rabbits

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