Treatment with pertussis toxin (PTX) which eliminates the activity of G(i) proteins effectively reduces blood pressure (BP) and vascular resistance in spontaneously hypertensive rats (SHR). In this study we have compared the functional characteristics of isolated arteries from SHR with and without PTX-treatment (10 microg/kg i.v., 48 h before the experiment). Rings of thoracic aorta, superior mesenteric artery and main pulmonary artery were studied under isometric conditions to measure the reactivity of these vessels to receptor agonists and to transmural electrical stimuli. We have found that the treatment of SHR with PTX had no effect on endothelium-dependent relaxation of thoracic aorta induced by acetylcholine. In PTX-treated SHR, the maximum contraction of mesenteric artery to exogenous noradrenaline was reduced and the dose-response curve to cumulative concentration of noradrenaline was shifted to the right. Similarly, a reduction in the magnitude of neurogenic contractions elicited by electrical stimulation of perivascular nerves was observed in the mesenteric artery from PTX-treated SHR. PTX treatment of SHR also abolished the potentiating effect of angiotensin II on neurogenic contractions of the main pulmonary artery. These results indicate that PTX treatment markedly diminishes the effectiveness of adrenergic stimuli in vasculature of SHR. This could importantly affect BP regulation in genetic hypertension.

Institute of Normal and Pathological Physiology Slovak Academy of Sciences Bratislava

Lit.: 16

000      

00000naa 2200000 a 4500

001      

bmc07519647

003      

CZ-PrNML

005      

20111210130848.0

008      

090319s2008 xr e eng||

009      

AR

024    7_

$a 10.33549/physiolres.931447 $2 doi

040    __

$a ABA008 $b cze $c ABA008 $d ABA008 $e AACR2

041    0_

$a eng

044    __

$a xr

100    1_

$a Zemančíková, Anna. $7 xx0240449

245    10

$a Inactivation of Gi proteins by pertussis toxin diminishes the effectiveness of adrenergic stimuli in conduit arteries from spontaneously hypertensive rats / $c Anna Zemančíková, Jozef Török, Josef Zicha, Jaroslav Kuneš

314    __

$a Institute of Normal and Pathological Physiology, Slovak Academy of Sciences, Bratislava

504    __

$a Lit.: 16

520    9_

$a Treatment with pertussis toxin (PTX) which eliminates the activity of G(i) proteins effectively reduces blood pressure (BP) and vascular resistance in spontaneously hypertensive rats (SHR). In this study we have compared the functional characteristics of isolated arteries from SHR with and without PTX-treatment (10 microg/kg i.v., 48 h before the experiment). Rings of thoracic aorta, superior mesenteric artery and main pulmonary artery were studied under isometric conditions to measure the reactivity of these vessels to receptor agonists and to transmural electrical stimuli. We have found that the treatment of SHR with PTX had no effect on endothelium-dependent relaxation of thoracic aorta induced by acetylcholine. In PTX-treated SHR, the maximum contraction of mesenteric artery to exogenous noradrenaline was reduced and the dose-response curve to cumulative concentration of noradrenaline was shifted to the right. Similarly, a reduction in the magnitude of neurogenic contractions elicited by electrical stimulation of perivascular nerves was observed in the mesenteric artery from PTX-treated SHR. PTX treatment of SHR also abolished the potentiating effect of angiotensin II on neurogenic contractions of the main pulmonary artery. These results indicate that PTX treatment markedly diminishes the effectiveness of adrenergic stimuli in vasculature of SHR. This could importantly affect BP regulation in genetic hypertension.

650    _2

$a acetylcholin $x metabolismus $7 D000109

650    _2

$a adrenergní látky $x metabolismus $7 D018663

650    _2

$a angiotensin II $x metabolismus $7 D000804

650    _2

$a zvířata $7 D000818

650    _2

$a aorta thoracica $x metabolismus $x účinky léků $7 D001013

650    _2

$a arterie $x metabolismus $x účinky léků $7 D001158

650    _2

$a krevní tlak $x genetika $x účinky záření $7 D001794

650    _2

$a elektrická stimulace $7 D004558

650    _2

$a cévní endotel $x metabolismus $x účinky léků $7 D004730

650    _2

$a inhibitory enzymů $x farmakologie $7 D004791

650    _2

$a proteiny vázající GTP - alfa-podjednotky Gi-Go $x antagonisté a inhibitory $x metabolismus $7 D019206

650    _2

$a hypertenze $x farmakoterapie $x metabolismus $7 D006973

650    _2

$a mužské pohlaví $7 D008297

650    _2

$a arteria mesenterica superior $x metabolismus $x účinky léků $7 D017538

650    _2

$a noradrenalin $x metabolismus $7 D009638

650    _2

$a pertusový toxin $x farmakologie $7 D037342

650    _2

$a arteria pulmonalis $x metabolismus $x účinky léků $7 D011651

650    _2

$a krysa rodu Rattus $7 D051381

650    _2

$a potkani inbrední SHR $7 D011918

650    _2

$a signální transdukce $x fyziologie $x účinky léků $7 D015398

700    1_

$a Török, Jozef. $7 xx0240443

700    1_

$a Zicha, Josef, $d 1950- $7 jk01152609

700    1_

$a Kuneš, Jaroslav, $d 1948- $7 nlk19990073450

773    0_

$w MED00003824 $t Physiological research $g Roč. 57, č. 2 (2008), s. 299-302 $x 0862-8408

856    41

$u http://www.biomed.cas.cz/physiolres/pdf/57/57_299.pdf $y plný text volně přístupný

910    __

$a ABA008 $b A 4120 $c 266 $y 9

990    __

$a 20090317144817 $b ABA008

991    __

$a 20090326113810 $b ABA008

999    __

$a ok $b bmc $g 637457 $s 490233

BAS    __

$a 3

BMC    __

$a 2008 $b 57 $c 2 $d 299-302 $i 0862-8408 $m Physiological research $x MED00003824

LZP    __

$a 2009-12/mkme