-
Je něco špatně v tomto záznamu ?
Growth strategy of the pathogenic yeast Cryptococcus neoformans submerged culture under different cultivation formats
V. Raclavský, V. Husičková, Z. Moráňová, M. Ohkusu, O. Fischer, J. Přeček, J. Trtková, K. Takeo, S. Kawamoto
Jazyk angličtina Země Česko
- MeSH
- Cryptococcus neoformans metabolismus patogenita růst a vývoj MeSH
- financování organizované MeSH
- kryptokokóza MeSH
- kultivační média metabolismus MeSH
- kultivační techniky metody MeSH
- kyslík metabolismus MeSH
- lidé MeSH
- mikrobiální viabilita MeSH
- Check Tag
- lidé MeSH
Growth patterns of Cryptococcus neoformans submerged culture in different culture volumes, intensity of agitation and types of sealing were evaluated to better understand the physiological role of hypoxia response in this yeast. When low intensity agitation was set at high culture volumes and air exchange between the cultivation vessel and external environment was not abolished completely, the cells proliferated slowly but steadily. On the other hand, when the intensity of agitation was high but the vessel was withheld from fresh air supply, the cells first proliferated rapidly, then arrested completely and finally died. Therefore, the central strategy of C. neoformans here seems to lie in its proliferation-rate adjustment to the available oxygen levels and not in its capacity to survive under anoxia. The data support the opinion that the cultures grown under limited aeration (even though not completely withheld from fresh air supply) are much closer to the real cryptococcal life in human tissues than conventional well-aerated exponential cultures.
Lit.: 10
- 000
- 00000naa 2200000 a 4500
- 001
- bmc09006397
- 003
- CZ-PrNML
- 005
- 20120726105703.0
- 008
- 091230s2009 xr e eng||
- 009
- AR
- 040 __
- $a ABA008 $b cze $c ABA008 $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xr
- 100 1_
- $a Raclavský, Vladislav $7 xx0046827
- 245 10
- $a Growth strategy of the pathogenic yeast Cryptococcus neoformans submerged culture under different cultivation formats / $c V. Raclavský, V. Husičková, Z. Moráňová, M. Ohkusu, O. Fischer, J. Přeček, J. Trtková, K. Takeo, S. Kawamoto
- 314 __
- $a Department of Microbiology, Faculty of Medicine and Dentistry, Palacký University, Olomouc
- 504 __
- $a Lit.: 10
- 520 9_
- $a Growth patterns of Cryptococcus neoformans submerged culture in different culture volumes, intensity of agitation and types of sealing were evaluated to better understand the physiological role of hypoxia response in this yeast. When low intensity agitation was set at high culture volumes and air exchange between the cultivation vessel and external environment was not abolished completely, the cells proliferated slowly but steadily. On the other hand, when the intensity of agitation was high but the vessel was withheld from fresh air supply, the cells first proliferated rapidly, then arrested completely and finally died. Therefore, the central strategy of C. neoformans here seems to lie in its proliferation-rate adjustment to the available oxygen levels and not in its capacity to survive under anoxia. The data support the opinion that the cultures grown under limited aeration (even though not completely withheld from fresh air supply) are much closer to the real cryptococcal life in human tissues than conventional well-aerated exponential cultures.
- 650 _2
- $a financování organizované $7 D005381
- 650 _2
- $a kryptokokóza $7 D003453
- 650 _2
- $a Cryptococcus neoformans $x metabolismus $x patogenita $x růst a vývoj $7 D003455
- 650 _2
- $a kultivační média $x metabolismus $7 D003470
- 650 _2
- $a kultivační techniky $x metody $7 D046508
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a mikrobiální viabilita $7 D050296
- 650 _2
- $a kyslík $x metabolismus $7 D010100
- 700 1_
- $a Husičková, Vendula. $7 _AN042512
- 700 1_
- $a Moráňová, Zuzana. $7 _AN042513
- 700 1_
- $a Ohkusu, M.
- 700 1_
- $a Fischer, Ondřej. $7 _AN042514
- 700 1_
- $a Přeček, Jan, $d 1985- $7 xx0154445
- 700 1_
- $a Trtková, Jitka. $7 _AN042516
- 700 1_
- $a Takeo, K.
- 700 1_
- $a Kawamoto, S.
- 773 0_
- $w MED00011005 $t Folia microbiologica $g Roč. 54, č. 4 (2009), s. 649-352 $x 0015-5632
- 910 __
- $a ABA008 $b A 2403 $c 561 $y 8
- 990 __
- $a 20091230090615 $b ABA008
- 991 __
- $a 20120726105742 $b ABA008
- 999 __
- $a ok $b bmc $g 702211 $s 564640
- BAS __
- $a 3
- BMC __
- $a 2009 $b 54 $c 4 $d 649-352 $i 0015-5632 $m Folia microbiologica $x MED00011005
- LZP __
- $a 2009-61/ipme