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Direct detection of Borrelia burgdorferi spirochetes in patients with early disseminated Lyme borreliosis
Katarína Schwarzová, Zina Košťanová, Katarína Holečková, Eva Špitalská, Vojtech Boldiš
Language English Country Czech Republic
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- MeSH
- Amplified Fragment Length Polymorphism Analysis methods utilization MeSH
- Borrelia burgdorferi immunology isolation & purification MeSH
- Child MeSH
- Adult MeSH
- Microscopy, Electron methods utilization MeSH
- Enzyme-Linked Immunosorbent Assay methods utilization MeSH
- Financing, Organized MeSH
- Immunoglobulin G immunology isolation & purification MeSH
- Immunoglobulin M immunology isolation & purification MeSH
- Blood immunology microbiology MeSH
- Culture Techniques methods instrumentation utilization MeSH
- Humans MeSH
- Lyme Disease diagnosis microbiology MeSH
- Cerebrospinal Fluid immunology microbiology MeSH
- Polymerase Chain Reaction methods utilization MeSH
- Synovial Fluid immunology microbiology MeSH
- Blotting, Western methods utilization MeSH
- Check Tag
- Child MeSH
- Adult MeSH
- Humans MeSH
The detection of spirochetes in 15 patients with clinically documented early disseminated LB has been analysed when using cultivation method of the plasma or the cerebrospinal fluid, electron microscopy, commercial Western blot and detecting the DNA of the pathogen in vitro cultures by PCR-RFLP. Spirochetes were isolated in eight blood and one cerebrospinal fluid culture samples. In seven cases (47%), previous serodiagnostic laboratory tests were negative. Borrelial DNA was detected by PCR in 67% patients (9 blood samples and 1 CSF sample). Using MseI restriction fragments of PCR products of the amplified rrf-rrl region, we identified Borrelia garinii (80%), one B. afzelii isolate and one B. burgdorferi s.s.
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Lit.: 14
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