-
Je něco špatně v tomto záznamu ?
Real time RT-PCR with a newly designed set of primers confirmed the presence of 2b and 2x/d myosin heavy chain mRNAs in the rat slow soleus muscle
J. Žurmanová, F. Půta, R. Stopková, T. Soukup
Jazyk angličtina Země Česko
NLK
Directory of Open Access Journals
od 1991
Free Medical Journals
od 1998
ProQuest Central
od 2005-01-01
Medline Complete (EBSCOhost)
od 2006-01-01
Nursing & Allied Health Database (ProQuest)
od 2005-01-01
Health & Medicine (ProQuest)
od 2005-01-01
ROAD: Directory of Open Access Scholarly Resources
od 1998
- Klíčová slova
- Rat slow soleus muscle, Rat fast EDL muscle, Myosin heavy chain isoforms, Real time RT-PCR,
- MeSH
- DNA primery MeSH
- financování organizované MeSH
- kosterní svaly chemie MeSH
- krysa rodu rattus MeSH
- messenger RNA analýza MeSH
- polymerázová řetězová reakce MeSH
- potkani inbrední LEW MeSH
- protein - isoformy MeSH
- svalová vlákna typu I chemie MeSH
- svalová vlákna typu II chemie MeSH
- těžké řetězce myosinu genetika MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- ženské pohlaví MeSH
- zvířata MeSH
In order to re-evaluate the presence and relative quantity of 2b and 2x/d myosin heavy chain (MyHC) transcripts in rat slow soleus muscle by using real time RT-PCR we have compared the available relevant cDNA sequences and designed a new set of primers having similar melting temperatures, matching separate MyHC exons in the regions of maximal differences in MyHC coding sequences, and containing G or C at the 3´-end. These also yielded PCR products of corresponding length, which is an important requirement for real time RT-PCR quantification. The experiments were performed on 8-month-old inbred female Lewis strain rats used in our current study of regenerating transplanted muscles. The real time RT-PCR measurement confirmed the expression of all four MyHC mRNAs (type 1, 2a, 2x/d and 2b) in both fast extensor digitorum longus and slow soleus muscles, although in the soleus muscle of adult rats, only type 1 and 2a protein isoforms can be usually detected.
Citace poskytuje Crossref.org
Lit.: 26
- 000
- 00000naa 2200000 a 4500
- 001
- bmc10015759
- 003
- CZ-PrNML
- 005
- 20111210180601.0
- 008
- 100726s2008 xr e eng||
- 009
- AR
- 024 7_
- $a 10.33549/physiolres.931676 $2 doi
- 040 __
- $a ABA008 $b cze $c ABA008 $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xr
- 100 1_
- $a Žurmanová, Jitka. $7 uk2008403153
- 245 10
- $a Real time RT-PCR with a newly designed set of primers confirmed the presence of 2b and 2x/d myosin heavy chain mRNAs in the rat slow soleus muscle / $c J. Žurmanová, F. Půta, R. Stopková, T. Soukup
- 314 __
- $a Institute of Physiology, AS ČR, Prague
- 504 __
- $a Lit.: 26
- 520 9_
- $a In order to re-evaluate the presence and relative quantity of 2b and 2x/d myosin heavy chain (MyHC) transcripts in rat slow soleus muscle by using real time RT-PCR we have compared the available relevant cDNA sequences and designed a new set of primers having similar melting temperatures, matching separate MyHC exons in the regions of maximal differences in MyHC coding sequences, and containing G or C at the 3´-end. These also yielded PCR products of corresponding length, which is an important requirement for real time RT-PCR quantification. The experiments were performed on 8-month-old inbred female Lewis strain rats used in our current study of regenerating transplanted muscles. The real time RT-PCR measurement confirmed the expression of all four MyHC mRNAs (type 1, 2a, 2x/d and 2b) in both fast extensor digitorum longus and slow soleus muscles, although in the soleus muscle of adult rats, only type 1 and 2a protein isoforms can be usually detected.
- 650 _2
- $a financování organizované $7 D005381
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a DNA primery $7 D017931
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a svalová vlákna typu II $x chemie $7 D018656
- 650 _2
- $a svalová vlákna typu I $x chemie $7 D018657
- 650 _2
- $a kosterní svaly $x chemie $7 D018482
- 650 _2
- $a těžké řetězce myosinu $x genetika $7 D018995
- 650 _2
- $a polymerázová řetězová reakce $7 D016133
- 650 _2
- $a protein - isoformy $7 D020033
- 650 _2
- $a messenger RNA $x analýza $7 D012333
- 650 _2
- $a krysa rodu Rattus $7 D051381
- 650 _2
- $a potkani inbrední LEW $7 D011917
- 653 00
- $a Rat slow soleus muscle
- 653 00
- $a Rat fast EDL muscle
- 653 00
- $a Myosin heavy chain isoforms
- 653 00
- $a Real time RT-PCR
- 700 1_
- $a Půta, František $7 xx0093597
- 700 1_
- $a Stopková, Romana. $7 _AN038971
- 700 1_
- $a Soukup, Tomáš $7 xx0140789
- 773 0_
- $w MED00003824 $t Physiological research $g Roč. 57, č. 6 (2008), s. 973-978 $x 0862-8408
- 856 41
- $u http://www.biomed.cas.cz/physiolres/pdf/57/57_973.pdf $y plný text volně přístupný
- 910 __
- $a ABA008 $b A 4120 $c 266 $y 8
- 990 __
- $a 20100708110758 $b ABA008
- 991 __
- $a 20100817085507 $b ABA008
- 999 __
- $a ok $b bmc $g 756174 $s 619957
- BAS __
- $a 3
- BMC __
- $a 2008 $b 57 $c 6 $m Physiological research $x MED00003824 $d 973-978
- LZP __
- $a 2010-32/vtme
