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The ability of the rumen protozoan Eudiplodinium maggii to utilize chitin
R. Miltko, G. Belzecki, E. Kwiatkowska, T. Michalowski
Jazyk angličtina Země Česko
- MeSH
- acetylglukosamin metabolismus MeSH
- bachor parazitologie MeSH
- chitin izolace a purifikace metabolismus MeSH
- Ciliophora izolace a purifikace metabolismus MeSH
- financování organizované MeSH
- houby chemie MeSH
- kultivační média chemie MeSH
- kyselina máselná metabolismus MeSH
- kyselina octová metabolismus MeSH
- kyseliny mastné těkavé metabolismus MeSH
- ovce MeSH
- propionáty metabolismus MeSH
- trisacharidy metabolismus MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
The ability was determined of the rumen ciliate Eudiplodinium maggii to utilize chitin from fungal cell wall. Cultivation experiments shoved that the population concentration (number of ciliates in vitro) was positively correlated with chitin doses. Cell extract prepared from the bacteria-free ciliates degraded colloidal chitin releasing 2.0 micromol reducing sugar per mg protein per h. End products of this reaction were chitotriose and N-acetylglucosamine. Incubation of the bacteria-free ciliates with chitin resulted in an increase in the concentration of acetic, propionic and butyric acids in the incubation medium. The production rate of total volatile fatty acids (VFA) by ciliates incubated with and without chitin was 45.0 and 30.5 pmol VFA per protozoan, respectively, the molar proportion of particular acids remaining unchanged.
6th International Symposium of Anaerobic Microbiology (ISAM 2009) held in Liblice (Czech Republic), 18 - 20 June 2009
Bibliografie atd.Lit.: 17
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- $a The Kielanowski Institute of Animal Physiology and Nutrition Polish Academy of Sciences, Jabłonna, Poland r.miltko@ifzz.pan.pl
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- $a 6th International Symposium of Anaerobic Microbiology (ISAM 2009) held in Liblice (Czech Republic), 18 - 20 June 2009
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- $a The ability was determined of the rumen ciliate Eudiplodinium maggii to utilize chitin from fungal cell wall. Cultivation experiments shoved that the population concentration (number of ciliates in vitro) was positively correlated with chitin doses. Cell extract prepared from the bacteria-free ciliates degraded colloidal chitin releasing 2.0 micromol reducing sugar per mg protein per h. End products of this reaction were chitotriose and N-acetylglucosamine. Incubation of the bacteria-free ciliates with chitin resulted in an increase in the concentration of acetic, propionic and butyric acids in the incubation medium. The production rate of total volatile fatty acids (VFA) by ciliates incubated with and without chitin was 45.0 and 30.5 pmol VFA per protozoan, respectively, the molar proportion of particular acids remaining unchanged.
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