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A determination of submicromolar concentrations of glycine in periaqueductal gray matter microdialyzates using capillary zone electrophoresis with contactless conductivity detection
P Tuma, M Soukupova, E Samcova, K Stulik
Jazyk angličtina Země Německo
Typ dokumentu práce podpořená grantem
NLK
Wiley Online Library (archiv)
od 1999-01-01 do 2012-12-31
- MeSH
- bolest farmakoterapie chemicky indukované MeSH
- elektrická vodivost MeSH
- elektroforéza kapilární metody MeSH
- glycin analýza metabolismus MeSH
- karagenan farmakologie MeSH
- krysa rodu rattus MeSH
- neopioidní analgetika farmakologie MeSH
- paracetamol farmakologie MeSH
- potkani Wistar MeSH
- reprodukovatelnost výsledků MeSH
- senzitivita a specificita MeSH
- substantia grisea centralis chemie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- práce podpořená grantem MeSH
CE with contactless conductivity detection has been used to determine the glycine neurotransmitter in periaqueductal gray matter (PAG) of rats. The LOD for glycine has been decreased to a value of 0.2 microM by adding 75% v/v of ACN to the samples and increasing the sample zone introduced to a value of 20% of the overall capillary length. The repeatabilities of the analyte migration times and the zone areas amount to 2.1 and 2.7%, respectively. The optimized CE/contactless conductivity detection method makes it possible to determine the micromolar concentrations of glycine in PAG microdialyzates without the necessity of sample derivatization. It follows from a pharmacological study that a local inflammation initiated by an application of carrageenan increased the glycine concentration in the rat PAG seven times, compared with a control. The glycine level in PAG can be decreased and the pain suppressed by administering paracetamol.
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- $a Tůma, Petr $7 xx0105786 $u Institute of Biochemistry, Cell and Molecular Biology, Third Faculty of Medicine, Charles University, Czech Republic. petr.tuma@lf3.cuni.cz
- 245 12
- $a A determination of submicromolar concentrations of glycine in periaqueductal gray matter microdialyzates using capillary zone electrophoresis with contactless conductivity detection / $c P Tuma, M Soukupova, E Samcova, K Stulik
- 520 9_
- $a CE with contactless conductivity detection has been used to determine the glycine neurotransmitter in periaqueductal gray matter (PAG) of rats. The LOD for glycine has been decreased to a value of 0.2 microM by adding 75% v/v of ACN to the samples and increasing the sample zone introduced to a value of 20% of the overall capillary length. The repeatabilities of the analyte migration times and the zone areas amount to 2.1 and 2.7%, respectively. The optimized CE/contactless conductivity detection method makes it possible to determine the micromolar concentrations of glycine in PAG microdialyzates without the necessity of sample derivatization. It follows from a pharmacological study that a local inflammation initiated by an application of carrageenan increased the glycine concentration in the rat PAG seven times, compared with a control. The glycine level in PAG can be decreased and the pain suppressed by administering paracetamol.
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