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A determination of submicromolar concentrations of glycine in periaqueductal gray matter microdialyzates using capillary zone electrophoresis with contactless conductivity detection
P Tuma, M Soukupova, E Samcova, K Stulik
Language English Country Germany
Document type Research Support, Non-U.S. Gov't
NLK
Wiley Online Library (archiv)
from 1999-01-01 to 2012-12-31
- MeSH
- Pain drug therapy chemically induced MeSH
- Electric Conductivity MeSH
- Electrophoresis, Capillary methods MeSH
- Glycine analysis metabolism MeSH
- Carrageenan pharmacology MeSH
- Rats MeSH
- Analgesics, Non-Narcotic pharmacology MeSH
- Acetaminophen pharmacology MeSH
- Rats, Wistar MeSH
- Reproducibility of Results MeSH
- Sensitivity and Specificity MeSH
- Periaqueductal Gray chemistry MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Research Support, Non-U.S. Gov't MeSH
CE with contactless conductivity detection has been used to determine the glycine neurotransmitter in periaqueductal gray matter (PAG) of rats. The LOD for glycine has been decreased to a value of 0.2 microM by adding 75% v/v of ACN to the samples and increasing the sample zone introduced to a value of 20% of the overall capillary length. The repeatabilities of the analyte migration times and the zone areas amount to 2.1 and 2.7%, respectively. The optimized CE/contactless conductivity detection method makes it possible to determine the micromolar concentrations of glycine in PAG microdialyzates without the necessity of sample derivatization. It follows from a pharmacological study that a local inflammation initiated by an application of carrageenan increased the glycine concentration in the rat PAG seven times, compared with a control. The glycine level in PAG can be decreased and the pain suppressed by administering paracetamol.
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- $a CE with contactless conductivity detection has been used to determine the glycine neurotransmitter in periaqueductal gray matter (PAG) of rats. The LOD for glycine has been decreased to a value of 0.2 microM by adding 75% v/v of ACN to the samples and increasing the sample zone introduced to a value of 20% of the overall capillary length. The repeatabilities of the analyte migration times and the zone areas amount to 2.1 and 2.7%, respectively. The optimized CE/contactless conductivity detection method makes it possible to determine the micromolar concentrations of glycine in PAG microdialyzates without the necessity of sample derivatization. It follows from a pharmacological study that a local inflammation initiated by an application of carrageenan increased the glycine concentration in the rat PAG seven times, compared with a control. The glycine level in PAG can be decreased and the pain suppressed by administering paracetamol.
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