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Simultaneous genotyping of microsatellite variations in HMOX1 and UGT1A1 genes using multicolored capillary electrophoresis
A. Jiraskova, M. Lenicek, L. Vitek
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- elektroforéza kapilární metody MeSH
- genotyp MeSH
- glukuronosyltransferasa genetika MeSH
- hemoxygenasa-1 genetika MeSH
- jednonukleotidový polymorfismus genetika MeSH
- lidé MeSH
- mikrosatelitní repetice genetika MeSH
- reprodukovatelnost výsledků MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
OBJECTIVES: Our aim was to establish a reliable, rapid, and inexpensive method for the simultaneous genotyping of the HMOX-1 (heme oxygenase-1) and UGT1A1 (bilirubin UDP-glucuronosyltransferase) gene promoter variations. RESULTS: The HMOX1 (GT)(n) and UGT1A1 (TA)(n) gene promoter variations were determined by fragment analysis using a single duplex PCR, with different fluorescent dye-labeled primers; followed by multicolored capillary electrophoresis. CONCLUSION: This novel method provides simultaneous genotyping of key tandem repeat variations in the HMOX1 and UGT1A1 promoters.
Citace poskytuje Crossref.org
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- $a 10.1016/j.clinbiochem.2010.01.006 $2 doi
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- $a Jirásková, Alena. $7 _BN007805 $u Department of Internal Medicine, Charles University in Prague, Czech Republic. alena@biomed.cas.cz
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- $a OBJECTIVES: Our aim was to establish a reliable, rapid, and inexpensive method for the simultaneous genotyping of the HMOX-1 (heme oxygenase-1) and UGT1A1 (bilirubin UDP-glucuronosyltransferase) gene promoter variations. RESULTS: The HMOX1 (GT)(n) and UGT1A1 (TA)(n) gene promoter variations were determined by fragment analysis using a single duplex PCR, with different fluorescent dye-labeled primers; followed by multicolored capillary electrophoresis. CONCLUSION: This novel method provides simultaneous genotyping of key tandem repeat variations in the HMOX1 and UGT1A1 promoters.
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