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Tyrosine- and histidine-decarboxylase positive lactic acid bacteria and enterococci in dry fermented sausages
T. Komprda, P. Sládková, E. Petirová, V. Dohnal, R. Burdychová
Language English Country England, Great Britain
Document type Journal Article
- MeSH
- Biogenic Amines biosynthesis MeSH
- Enterococcus genetics isolation & purification metabolism MeSH
- Fermentation MeSH
- Histidine Decarboxylase analysis genetics MeSH
- Lactobacillus genetics isolation & purification metabolism MeSH
- Meat Products microbiology MeSH
- Food Microbiology MeSH
- Base Sequence MeSH
- Sequence Analysis, DNA MeSH
- Tyrosine Decarboxylase analysis genetics MeSH
- Publication type
- Journal Article MeSH
Lactic acid bacteria (LAB) and enterococci were isolated immediately after stuffing (day 0), at the end of ripening (28th day) and at the end of storage (112th day) from dry fermented sausages produced by two different producers (K; R) in two diameters (4.5 and 7 cm) using either of two spice mixtures (P; H) and either of two starter cultures (Pediococcus pentosaceus, C; Lactobacillus curvatus+Staphylococcus carnosus, F), resulting in a total of 16 different combinations. Tyrosine-decarboxylase DNA sequence (tyrdc) was identified on average in 88% and 44% of enterococci and LAB isolates, respectively at the end of ripening, the corresponding figures regarding histidine-decarboxylase gene sequence (hisdc) was 71% and 16%, respectively. Lactobacillus plantarum, L. brevis and L. casei/paracasei, and Enterococcus faecium and Enterococcus faecalis were identified as tyramine/histamine producers in the sausages.
Department of Chemistry University of J E Purkynje in Usti nad Labem Czech Republic
Department of Food Technology Mendel University Brno Czech Republic
References provided by Crossref.org
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- $a Lactic acid bacteria (LAB) and enterococci were isolated immediately after stuffing (day 0), at the end of ripening (28th day) and at the end of storage (112th day) from dry fermented sausages produced by two different producers (K; R) in two diameters (4.5 and 7 cm) using either of two spice mixtures (P; H) and either of two starter cultures (Pediococcus pentosaceus, C; Lactobacillus curvatus+Staphylococcus carnosus, F), resulting in a total of 16 different combinations. Tyrosine-decarboxylase DNA sequence (tyrdc) was identified on average in 88% and 44% of enterococci and LAB isolates, respectively at the end of ripening, the corresponding figures regarding histidine-decarboxylase gene sequence (hisdc) was 71% and 16%, respectively. Lactobacillus plantarum, L. brevis and L. casei/paracasei, and Enterococcus faecium and Enterococcus faecalis were identified as tyramine/histamine producers in the sausages.
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