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In vitro testing of gentamicin-vancomycin loaded bone cement to prevent prosthetic joint infection
J. Gallo, M. Kolár, AV. Florschütz, R. Novotný, R. Pantůcek, M. Kesselová
Jazyk angličtina Země Česko
Typ dokumentu techniky in vitro, časopisecké články, práce podpořená grantem
Grantová podpora
NM7680
MZ0
CEP - Centrální evidence projektů
Digitální knihovna NLK
Plný text - Část
Zdroj
Free Medical Journals od 1998
ROAD: Directory of Open Access Scholarly Resources od 2001
- MeSH
- antibakteriální látky aplikace a dávkování farmakologie MeSH
- artroplastiky kloubů škodlivé účinky MeSH
- biofilmy růst a vývoj MeSH
- gentamiciny aplikace a dávkování farmakologie MeSH
- infekce spojené s protézou prevence a kontrola MeSH
- kostní cementy MeSH
- lidé MeSH
- mikrobiální testy citlivosti MeSH
- polyethyleny MeSH
- polymethylmethakrylát MeSH
- protézy kloubů MeSH
- Staphylococcus růst a vývoj MeSH
- vankomycin aplikace a dávkování farmakologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- techniky in vitro MeSH
Sepsis is a greatly feared complication of total joint arthroplasty. One key question is how to prevent perioperative bacterial adherence, and therefore the potential for infectious complications. The objective of our study was to appraise the emerging capacity of staphylococcal survival on prosthetic materials and to analyze the in vitro effects of gentamicin and vancomycin loaded polymethylmethacrylate (PMMA) cement on bacterial adherence and growth. Hospital acquired staphylococcal strains were systematically inoculated on four orthopedic materials (ultrahigh molecular weight polyethylene, PMMA without antibiotic, commercially produced PMMA loaded with gentamicin, and manually mixed PMMA loaded with gentamicin and vancomycin). Staphylococci were identified using culture and biochemical tests. The inoculated material was allowed to incubate in a liquid broth growth media and subsequently prepared for scanning electron microscopy and bacterial growth quantification. Materials without antibiotics showed evidence of staphylococcal growth. PMMA loaded with only gentamicin grew methicillin-resistant Staphylococcus aureus. Gentamicin-vancomycin loaded PMMA completely inhibited any bacterial growth. Low-dose gentamicin-vancomycin loaded PMMA prevents staphylococcal colonization better than commercially manufactured PMMA loaded with gentamicin. We recommend this combination in high-risk procedures and revision surgeries requiring bone cement.
Currently affiliated with the Department of Orthopaedics Mayo Clinic Rochester MN USA
Department of Genetics and Molecular Biology Faculty of Science Masaryk University Brno
Department of Microbiology Palacky University Olomouc
Department of Microscopic Methods Palacky University Olomouc
Department of Orthopaedics Palacký University Teaching Hospital Olomouc
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- $a Sepsis is a greatly feared complication of total joint arthroplasty. One key question is how to prevent perioperative bacterial adherence, and therefore the potential for infectious complications. The objective of our study was to appraise the emerging capacity of staphylococcal survival on prosthetic materials and to analyze the in vitro effects of gentamicin and vancomycin loaded polymethylmethacrylate (PMMA) cement on bacterial adherence and growth. Hospital acquired staphylococcal strains were systematically inoculated on four orthopedic materials (ultrahigh molecular weight polyethylene, PMMA without antibiotic, commercially produced PMMA loaded with gentamicin, and manually mixed PMMA loaded with gentamicin and vancomycin). Staphylococci were identified using culture and biochemical tests. The inoculated material was allowed to incubate in a liquid broth growth media and subsequently prepared for scanning electron microscopy and bacterial growth quantification. Materials without antibiotics showed evidence of staphylococcal growth. PMMA loaded with only gentamicin grew methicillin-resistant Staphylococcus aureus. Gentamicin-vancomycin loaded PMMA completely inhibited any bacterial growth. Low-dose gentamicin-vancomycin loaded PMMA prevents staphylococcal colonization better than commercially manufactured PMMA loaded with gentamicin. We recommend this combination in high-risk procedures and revision surgeries requiring bone cement.
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