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In vitro transfection mediated by dendrigraft poly(L-lysines): the effect of structure and molecule size
J. Hofman, M. Buncek, R. Haluza, L. Streinz, M. Ledvina, P. Cigler,
Jazyk angličtina Země Německo
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
23233456
DOI
10.1002/mabi.201200303
Knihovny.cz E-zdroje
- MeSH
- buněčné linie MeSH
- deoxyribonukleasa I metabolismus MeSH
- lidé MeSH
- lipidy MeSH
- malá interferující RNA MeSH
- molekulární sekvence - údaje MeSH
- oligonukleotidy metabolismus farmakokinetika MeSH
- plazmidy farmakokinetika MeSH
- polylysin chemie farmakokinetika MeSH
- sekvence nukleotidů MeSH
- transfekce metody MeSH
- viabilita buněk účinky léků MeSH
- vztahy mezi strukturou a aktivitou MeSH
- zelené fluorescenční proteiny genetika MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Dendritic poly(L-lysines) (DGL) constitute promising nanomaterials applicable as a nonviral gene-delivery vector. In this study, we evaluate the transfection abilities of four DGL generations with special emphasis on the systematic description of the relationship of how generation (i.e., molecule size) affects the transfection efficacy. Using Hep2 cells, we demonstrated that the capability of unmodified DGL to deliver plasmid is of a magnitude lower than that of jetPEI. On the other hand, employing the Hep2 cell line stably transduced with eGFP, we observed that DGL G5 delivers the siRNA oligonucleotide with the same efficiency as Lipofectamine 2000. In further experiments, it was shown that DGL affords excellent ability to bind DNA, protect it against DNase I attack, and internalize it into cells.
Citace poskytuje Crossref.org
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