-
Je něco špatně v tomto záznamu ?
NKD1 marks intestinal and liver tumors linked to aberrant Wnt signaling
J. Stancikova, M. Krausova, M. Kolar, B. Fafilek, J. Svec, R. Sedlacek, M. Neroldova, J. Dobes, M. Horazna, L. Janeckova, M. Vojtechova, M. Oliverius, M. Jirsa, V. Korinek,
Jazyk angličtina Země Anglie, Velká Británie
Typ dokumentu časopisecké články, práce podpořená grantem
Odkazy
PubMed
25446263
DOI
10.1016/j.cellsig.2014.11.008
Knihovny.cz E-zdroje
- MeSH
- beta-katenin genetika metabolismus MeSH
- genetická transkripce MeSH
- hepatocelulární karcinom metabolismus patologie MeSH
- hepatocyty metabolismus patologie MeSH
- kolorektální nádory metabolismus patologie MeSH
- lidé MeSH
- messenger RNA metabolismus MeSH
- mutace MeSH
- myši transgenní MeSH
- myši MeSH
- nádory jater metabolismus patologie MeSH
- protein familiární adenomatózní polypózy nedostatek genetika metabolismus MeSH
- proteiny Wnt metabolismus MeSH
- signální transdukce MeSH
- střevní nádory metabolismus patologie MeSH
- transportní proteiny genetika metabolismus MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The activity of the Wnt pathway undergoes complex regulation to ensure proper functioning of this principal signaling mechanism during development of adult tissues. The regulation may occur at several levels and includes both positive and negative feedback loops. In the present study we employed one of such negative feedback regulators, naked cuticle homolog 1 (Nkd1), to follow the Wnt pathway activity in the intestine and liver and in neoplasia originated in these organs. Using lineage tracing in transgenic mice we localized Nkd1 mRNA to the bottom parts of the small intestinal crypts and hepatocytes surrounding the central vein of the hepatic lobule. Furthermore, in two mouse models of intestinal tumorigenesis, Nkd1 expression levels were elevated in tumors when compared to healthy tissue. We utilized a collection of human intestinal polyps and carcinomas to confirm that NKD1 represents a robust marker of neoplastic growth. In addition, expression analysis of NKD1 in liver cancer showed that high expression levels of the gene distinguish a subclass of hepatocellular carcinomas related to aberrant Wnt signaling. Finally, our results were confirmed by bioinformatic analysis of large publicly available datasets that included gene expression profiling and high-throughput sequencing data of human colon and liver cancer specimens.
Faculty of Science Charles University Prague Albertov 6 128 43 Praha 2 Czech Republic
Institute for Clinical and Experimental Medicine Videnska 1958 9 140 21 Prague 4 Czech Republic
- 000
- 00000naa a2200000 a 4500
- 001
- bmc15031672
- 003
- CZ-PrNML
- 005
- 20151007120322.0
- 007
- ta
- 008
- 151005s2015 enk f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.cellsig.2014.11.008 $2 doi
- 035 __
- $a (PubMed)25446263
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a enk
- 100 1_
- $a Stancikova, Jitka $u Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083142 20 Prague 4, Czech Republic; Faculty of Science, Charles University in Prague, Albertov 6, 128 43 Praha 2, Czech Republic.
- 245 10
- $a NKD1 marks intestinal and liver tumors linked to aberrant Wnt signaling / $c J. Stancikova, M. Krausova, M. Kolar, B. Fafilek, J. Svec, R. Sedlacek, M. Neroldova, J. Dobes, M. Horazna, L. Janeckova, M. Vojtechova, M. Oliverius, M. Jirsa, V. Korinek,
- 520 9_
- $a The activity of the Wnt pathway undergoes complex regulation to ensure proper functioning of this principal signaling mechanism during development of adult tissues. The regulation may occur at several levels and includes both positive and negative feedback loops. In the present study we employed one of such negative feedback regulators, naked cuticle homolog 1 (Nkd1), to follow the Wnt pathway activity in the intestine and liver and in neoplasia originated in these organs. Using lineage tracing in transgenic mice we localized Nkd1 mRNA to the bottom parts of the small intestinal crypts and hepatocytes surrounding the central vein of the hepatic lobule. Furthermore, in two mouse models of intestinal tumorigenesis, Nkd1 expression levels were elevated in tumors when compared to healthy tissue. We utilized a collection of human intestinal polyps and carcinomas to confirm that NKD1 represents a robust marker of neoplastic growth. In addition, expression analysis of NKD1 in liver cancer showed that high expression levels of the gene distinguish a subclass of hepatocellular carcinomas related to aberrant Wnt signaling. Finally, our results were confirmed by bioinformatic analysis of large publicly available datasets that included gene expression profiling and high-throughput sequencing data of human colon and liver cancer specimens.
- 650 _2
- $a protein familiární adenomatózní polypózy $x nedostatek $x genetika $x metabolismus $7 D025601
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a hepatocelulární karcinom $x metabolismus $x patologie $7 D006528
- 650 _2
- $a transportní proteiny $x genetika $x metabolismus $7 D002352
- 650 _2
- $a kolorektální nádory $x metabolismus $x patologie $7 D015179
- 650 _2
- $a hepatocyty $x metabolismus $x patologie $7 D022781
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a střevní nádory $x metabolismus $x patologie $7 D007414
- 650 _2
- $a nádory jater $x metabolismus $x patologie $7 D008113
- 650 _2
- $a myši $7 D051379
- 650 _2
- $a myši transgenní $7 D008822
- 650 _2
- $a mutace $7 D009154
- 650 _2
- $a messenger RNA $x metabolismus $7 D012333
- 650 _2
- $a signální transdukce $7 D015398
- 650 _2
- $a genetická transkripce $7 D014158
- 650 _2
- $a proteiny Wnt $x metabolismus $7 D051153
- 650 _2
- $a beta-katenin $x genetika $x metabolismus $7 D051176
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Krausova, Michaela $u Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083142 20 Prague 4, Czech Republic.
- 700 1_
- $a Kolar, Michal $u Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083142 20 Prague 4, Czech Republic.
- 700 1_
- $a Fafilek, Bohumil $u Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083142 20 Prague 4, Czech Republic.
- 700 1_
- $a Svec, Jiri $u Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083142 20 Prague 4, Czech Republic; Second Department of Internal Medicine, Third Faculty of Medicine, Charles University, Prague, Srobarova 50, 100 34 Prague 10, Czech Republic.
- 700 1_
- $a Sedlacek, Radislav $u Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083142 20 Prague 4, Czech Republic.
- 700 1_
- $a Neroldova, Magdalena $u Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21 Prague 4, Czech Republic.
- 700 1_
- $a Dobes, Jan $u Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083142 20 Prague 4, Czech Republic.
- 700 1_
- $a Horazna, Monika $u Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083142 20 Prague 4, Czech Republic.
- 700 1_
- $a Janeckova, Lucie $u Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083142 20 Prague 4, Czech Republic.
- 700 1_
- $a Vojtechova, Martina $u Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083142 20 Prague 4, Czech Republic.
- 700 1_
- $a Oliverius, Martin $u Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21 Prague 4, Czech Republic.
- 700 1_
- $a Jirsa, Milan $u Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21 Prague 4, Czech Republic.
- 700 1_
- $a Korinek, Vladimir $u Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083142 20 Prague 4, Czech Republic. Electronic address: korinek@img.cas.cz.
- 773 0_
- $w MED00006628 $t Cellular signalling $x 1873-3913 $g Roč. 27, č. 2 (2015), s. 245-56
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/25446263 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20151005 $b ABA008
- 991 __
- $a 20151007120507 $b ABA008
- 999 __
- $a ok $b bmc $g 1092548 $s 914798
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2015 $b 27 $c 2 $d 245-56 $e 20141114 $i 1873-3913 $m Cellular signalling $n Cell Signal $x MED00006628
- LZP __
- $a Pubmed-20151005