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An efficient method for isolation of plasmid DNA from methylotrophic bacteria
V. Brenner, I. Holubová, J. Hubácek,
Jazyk angličtina Země Česko
Typ dokumentu časopisecké články
PubMed
2125292
Knihovny.cz E-zdroje
- MeSH
- DNA bakterií izolace a purifikace MeSH
- kruhová DNA izolace a purifikace MeSH
- methanol metabolismus MeSH
- Methylococcaceae genetika metabolismus MeSH
- oxidace-redukce MeSH
- plazmidy * MeSH
- půdní mikrobiologie * MeSH
- Publikační typ
- časopisecké články MeSH
A method, suitable for the isolation of closed circular plasmid DNA from methylotrophic bacteria is described. Improvement of cell lysis was achieved by butanol extraction of cells before application of the lytic agent. Using this method, cryptic plasmids of 7.8, 14, 36 and 200 kb were purified from soil-isolated methylotrophs.
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- $a A method, suitable for the isolation of closed circular plasmid DNA from methylotrophic bacteria is described. Improvement of cell lysis was achieved by butanol extraction of cells before application of the lytic agent. Using this method, cryptic plasmids of 7.8, 14, 36 and 200 kb were purified from soil-isolated methylotrophs.
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