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Anti-calreticulin immunoglobulin A (IgA) antibodies in refractory coeliac disease
D. Sánchez, L. Palová-Jelínková, J. Felsberg, M. Šimšová, A. Pekaríková, B. Pecharová, I. Swoboda, T. Mothes, C.J. Mulder, Z. Beneš, H. Tlaskalová-Hogenová, L. Tučková
Jazyk angličtina Země Velká Británie
E-zdroje NLK
Free Medical Journals od 1966 do Před 1 rokemPubMed Central od 1966 do Před 1 rokem
Medline Complete (EBSCOhost) od 1966-01-01 do Před 1 rokem
Wiley Online Library (archiv) od 1990-01-01 do 2012-12-31
- MeSH
- bezlepková dieta škodlivé účinky MeSH
- celiakie diagnóza imunologie krev MeSH
- ELISA MeSH
- enterocyty chemie imunologie MeSH
- financování organizované MeSH
- gliadin imunologie krev MeSH
- imunoglobulin A imunologie krev MeSH
- kalretikulin imunologie krev MeSH
- lidé středního věku MeSH
- lidé MeSH
- protilátky anti-idiotypické imunologie krev MeSH
- senioři MeSH
- senzitivita a specificita MeSH
- transglutaminasy imunologie krev MeSH
- western blotting MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
Refractory coeliac disease (RCD) is a very rare and dangerous form of CD, in which gluten-free diet loses its therapeutic effect and the damage of intestinal mucosa persists. Because of the adherence to the diet, serological markers of CD [immunoglobulin A (IgA) antibodies against gliadin, tissue transglutaminase (tTG) and endomysium] are often missing in RCD patients. We found substantially elevated levels of IgA anti-calreticulin (CRT) antibodies in the sera of almost all RCD patients tested. These sera were negative for IgA antibodies to gliadin and tTG and only some of them showed IgA antibodies to enterocytes. Analysis of patients' IgA reactivity to CRT fragments (quarters and halves) by Western blotting revealed differences in the specificity of IgA antibodies between RCD and CD patients. We therefore used the Pepscan technique with synthetic overlapping decapeptides of CRT to characterize antigenic epitopes recognized by serum IgA antibodies of RCD patients. Employing this method we demonstrated several dominant antigenic epitopes recognized by IgA antibodies of RCD patients on the CRT molecule. Epitope GVTKAAEKQMKD was recognized predominantly by serum IgA of RCD patients. Our results suggest that testing for serum IgA antibodies against CRT and its selected peptide could be a very useful tool in RCD differential diagnosis.
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- $a Department of Immunology, Institute of Microbiology, Czech Academy of Sciences, Prague, Czech Republic. sanchez.cohn@gmail.com
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- $a Refractory coeliac disease (RCD) is a very rare and dangerous form of CD, in which gluten-free diet loses its therapeutic effect and the damage of intestinal mucosa persists. Because of the adherence to the diet, serological markers of CD [immunoglobulin A (IgA) antibodies against gliadin, tissue transglutaminase (tTG) and endomysium] are often missing in RCD patients. We found substantially elevated levels of IgA anti-calreticulin (CRT) antibodies in the sera of almost all RCD patients tested. These sera were negative for IgA antibodies to gliadin and tTG and only some of them showed IgA antibodies to enterocytes. Analysis of patients' IgA reactivity to CRT fragments (quarters and halves) by Western blotting revealed differences in the specificity of IgA antibodies between RCD and CD patients. We therefore used the Pepscan technique with synthetic overlapping decapeptides of CRT to characterize antigenic epitopes recognized by serum IgA antibodies of RCD patients. Employing this method we demonstrated several dominant antigenic epitopes recognized by IgA antibodies of RCD patients on the CRT molecule. Epitope GVTKAAEKQMKD was recognized predominantly by serum IgA of RCD patients. Our results suggest that testing for serum IgA antibodies against CRT and its selected peptide could be a very useful tool in RCD differential diagnosis.
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