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Nitric oxide and oxidative stress is associated with severity of diabetic retinopathy and retinal structural alterations
S. Sharma, S. Saxena, K. Srivastav, RK. Shukla, N. Mishra, CH. Meyer, P. Kruzliak, VK. Khanna,
Language English Country Australia
Document type Journal Article, Observational Study
PubMed
25675974
DOI
10.1111/ceo.12506
Knihovny.cz E-resources
- MeSH
- Diabetes Mellitus, Type 2 complications MeSH
- Diabetic Retinopathy physiopathology MeSH
- Adult MeSH
- Glutathione blood MeSH
- Thiobarbituric Acid Reactive Substances metabolism MeSH
- Middle Aged MeSH
- Humans MeSH
- Lipid Peroxides blood MeSH
- Tomography, Optical Coherence MeSH
- Nitric Oxide blood MeSH
- Oxidative Stress * MeSH
- Lipid Peroxidation MeSH
- Prospective Studies MeSH
- Retinal Pigment Epithelium pathology MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Severity of Illness Index MeSH
- Retinal Photoreceptor Cell Inner Segment pathology MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Observational Study MeSH
BACKGROUND: The aim of the study was to determine plasma nitric oxide (NO) and lipid peroxide (LPO) levels in diabetic retinopathy and its association with severity of disease. DESIGN: Prospective observational study. PARTICIPANTS: A total of 60 consecutive cases and 20 healthy controls were included. METHODS: Severity of retinopathy was graded according to early treatment diabetic retinopathy study (ETDRS) classification. Photoreceptor inner segment ellipsoid band (ISel) disruption and retinal pigment epithelium (RPE) alteration were graded using spectral domain optical coherence tomography. Data were statistically analyzed. MAIN OUTCOME MEASURES: Plasma thiobarbituric acid reactive substances, NO assay and reduced glutathione (GSH) were measured using standard protocol. RESULTS: Increased severity of diabetic retinopathy was significantly associated with increase in plasma levels of LPO (P < 0.05), NO (P < 0.001) and decrease in plasma levels of GSH (P < 0.0001), ISel disruption (P < 0.001) and RPE topographic alteration (P < 0.01). CONCLUSION: Increased plasma NO levels are associated with increased severity of diabetic retinopathy. For the first time, it has been demonstrated that increased plasma LPO, NO and decreased GSH levels are associated with in vivo structural changes in inner segment ellipsoid and RPE.
Department of Ophthalmology Pallas Klinik Olten Switzerland
Developmental Toxicology Division Indian Institute of Toxicology and Research Lucknow India
Retina Service Department of Ophthalmology King George's Medical University Lucknow India
References provided by Crossref.org
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