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Genome-wide microRNA Expression Profiling in Primary Tumors and Matched Liver Metastasis of Patients with Colorectal Cancer

P. Vychytilova-Faltejskova, M. Pesta, L. Radova, V. Liska, O. Daum, Z. Kala, M. Svoboda, I. Kiss, O. Slaby,

. 2016 ; 13 (4) : 311-316.

Language English Country Greece

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc17013737

Grant support
NT13549 MZ0 CEP Register

BACKGROUND: Primary tumor spread to the liver is the major cause of disease progression and death in patients with colorectal cancer (CRC). MicroRNAs (miRNAs) are small non-coding RNAs that are involved in cancer development and progression, but their role in metastasis has not been extensively investigated. MATERIALS AND METHODS: Firstly, expression profiling of 752 miRNAs in 20 primary tumors and their corresponding liver metastases was performed. Secondly, validation of the results was carried out on an independent cohort of 66 patients with metastatic CRC using reverse transcription-quantitative polymerase chain (RT-qPCR) reaction. RESULTS: In total, 33 miRNAs were found to be significantly deregulated in liver metastases compared to their primary tumors. Fifteen miRNAs were chosen for subsequent validation, which confirmed significantly reduced expression of miR-143, miR 10b, and miR-28-5p, and increased expression of miR 122, miR-122*, and miR 885-5p in the tissue of liver metastases. CONCLUSION: These results indicate that miRNAs could serve as new therapeutic targets in patients with metastatic CRC.

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$a BACKGROUND: Primary tumor spread to the liver is the major cause of disease progression and death in patients with colorectal cancer (CRC). MicroRNAs (miRNAs) are small non-coding RNAs that are involved in cancer development and progression, but their role in metastasis has not been extensively investigated. MATERIALS AND METHODS: Firstly, expression profiling of 752 miRNAs in 20 primary tumors and their corresponding liver metastases was performed. Secondly, validation of the results was carried out on an independent cohort of 66 patients with metastatic CRC using reverse transcription-quantitative polymerase chain (RT-qPCR) reaction. RESULTS: In total, 33 miRNAs were found to be significantly deregulated in liver metastases compared to their primary tumors. Fifteen miRNAs were chosen for subsequent validation, which confirmed significantly reduced expression of miR-143, miR 10b, and miR-28-5p, and increased expression of miR 122, miR-122*, and miR 885-5p in the tissue of liver metastases. CONCLUSION: These results indicate that miRNAs could serve as new therapeutic targets in patients with metastatic CRC.
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$a Pešta, Martin, $u Department of Biology, Faculty of Medicine in Pilsen, Charles University in Prague, Prague, Czech Republic the Biomedical Center, Faculty of Medicine in Pilsen, Charles University in Prague, Prague, Czech Republic. $d 1976- $7 xx0073915
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$a Radova, Lenka $u Central European Institute of Technology, Masaryk University, Brno, Czech Republic.
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$a Liška, Václav, $u the Biomedical Center, Faculty of Medicine in Pilsen, Charles University in Prague, Prague, Czech Republic Department of Surgery, Faculty of Medicine in Pilsen, Charles University in Prague, Prague, Czech Republic. $d 1978- $7 xx0073943
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$a Daum, Ondřej, $u Department of Pathology, Faculty of Medicine in Pilsen, Charles University in Prague, Prague, Czech Republic. $d 1974- $7 xx0063668
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$a Kala, Zdeněk, $u Department of Surgery, University Hospital Brno, Faculty of Medicine, Masaryk University, Brno, Czech Republic. $d 1960- $7 xx0003702
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$a Svoboda, Marek, $u Central European Institute of Technology, Masaryk University, Brno, Czech Republic Department of Comprehensive Cancer Care, Masaryk Memorial Cancer Institute, Brno, Czech Republic. $d 1975- $7 xx0098478
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$a Kiss, Igor, $u Department of Comprehensive Cancer Care, Masaryk Memorial Cancer Institute, Brno, Czech Republic on.slaby@gmail.com kiss@mou.cz. $d 1965- $7 xx0031864
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$a Slabý, Ondřej, $u Central European Institute of Technology, Masaryk University, Brno, Czech Republic Department of Comprehensive Cancer Care, Masaryk Memorial Cancer Institute, Brno, Czech Republic on.slaby@gmail.com kiss@mou.cz. $d 1981- $7 js20030220015
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