Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Characterization of host-dependent mutations of apple fruit crinkle viroid replicating in newly identified experimental hosts suggests maintenance of stem-loop structures in the left-hand half of the molecule is important for replication

T. Suzuki, M. Fujibayashi, T. Hataya, A. Taneda, YH. He, T. Tsushima, GS. Duraisamy, K. Siglová, J. Matoušek, T. Sano,

. 2017 ; 98 (3) : 506-516. [pub] 20170401

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc17023415

Apple fruit crinkle viroid (AFCVd) is a tentative member of the genus Apscaviroid, family Pospiviroidae. AFCVd has a narrow host range and is known to infect apple, hop and persimmon as natural hosts. In this study, tomato, cucumber and wild hop have been identified as new experimental herbaceous hosts. Foliar symptoms were very mild or virtually undetectable, but fruits of infected tomato were small, cracked and distorted. These symptoms resemble those observed on some AFCVd-sensitive apple cultivars. After transfer to tomato, cucumber and wild hop, sequence changes were detected in a natural AFCVd isolate from hop, and major variants in tomato, cucumber and wild hop differed in 10, 8 or 2 nucleotides, respectively, from the predominant one in the inoculum. The major variants in tomato and cucumber were almost identical, and the one in wild hop was very similar to the one in cultivated hop. Detailed analyses of the host-dependent sequence changes that appear in a naturally occurring AFCVd isolate from hop after transfer to tomato using small RNA deep sequence data and infectivity studies with dimeric RNA transcripts followed by progeny analysis indicate that the major AFCVd variant in tomato emerged by selection of a minor variant present in the inoculum (i.e. hop) followed by one to two host-dependent de novo mutations. Comparison of the secondary structures of major variants in hop, tomato and persimmon after transfer to tomato suggested that maintenance of stem-loop structures in the left-hand half of the molecule is critical for infection.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc17023415
003      
CZ-PrNML
005      
20170831103107.0
007      
ta
008      
170720s2017 enk f 000 0|eng||
009      
AR
024    7_
$a 10.1099/jgv.0.000693 $2 doi
035    __
$a (PubMed)28005527
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Suzuki, Takahiro $u 1​Faculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, Hirosaki 036-8561, Japan.
245    10
$a Characterization of host-dependent mutations of apple fruit crinkle viroid replicating in newly identified experimental hosts suggests maintenance of stem-loop structures in the left-hand half of the molecule is important for replication / $c T. Suzuki, M. Fujibayashi, T. Hataya, A. Taneda, YH. He, T. Tsushima, GS. Duraisamy, K. Siglová, J. Matoušek, T. Sano,
520    9_
$a Apple fruit crinkle viroid (AFCVd) is a tentative member of the genus Apscaviroid, family Pospiviroidae. AFCVd has a narrow host range and is known to infect apple, hop and persimmon as natural hosts. In this study, tomato, cucumber and wild hop have been identified as new experimental herbaceous hosts. Foliar symptoms were very mild or virtually undetectable, but fruits of infected tomato were small, cracked and distorted. These symptoms resemble those observed on some AFCVd-sensitive apple cultivars. After transfer to tomato, cucumber and wild hop, sequence changes were detected in a natural AFCVd isolate from hop, and major variants in tomato, cucumber and wild hop differed in 10, 8 or 2 nucleotides, respectively, from the predominant one in the inoculum. The major variants in tomato and cucumber were almost identical, and the one in wild hop was very similar to the one in cultivated hop. Detailed analyses of the host-dependent sequence changes that appear in a naturally occurring AFCVd isolate from hop after transfer to tomato using small RNA deep sequence data and infectivity studies with dimeric RNA transcripts followed by progeny analysis indicate that the major AFCVd variant in tomato emerged by selection of a minor variant present in the inoculum (i.e. hop) followed by one to two host-dependent de novo mutations. Comparison of the secondary structures of major variants in hop, tomato and persimmon after transfer to tomato suggested that maintenance of stem-loop structures in the left-hand half of the molecule is critical for infection.
650    _2
$a Cucumis sativus $x virologie $7 D018553
650    _2
$a Diospyros $x virologie $7 D029788
650    _2
$a interakce hostitele a patogenu $7 D054884
650    _2
$a Humulus $x virologie $7 D027582
650    _2
$a obrácené repetice $7 D055029
650    _2
$a Solanum lycopersicum $x virologie $7 D018551
650    _2
$a mutace $7 D009154
650    _2
$a konformace nukleové kyseliny $7 D009690
650    _2
$a nemoci rostlin $x virologie $7 D010935
650    _2
$a rostlinné viry $x genetika $x izolace a purifikace $x fyziologie $7 D010942
650    _2
$a RNA virová $x chemie $x genetika $7 D012367
650    _2
$a viroidy $x genetika $x izolace a purifikace $x fyziologie $7 D014772
650    12
$a replikace viru $7 D014779
655    _2
$a časopisecké články $7 D016428
700    1_
$a Fujibayashi, Misato $u 1​Faculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, Hirosaki 036-8561, Japan.
700    1_
$a Hataya, Tatsuji $u 2​Laboratory of Pathogen-Plant Interactions, Research Faculty of Agriculture, Hokkaido University, Kita-ku, Sapporo 060-8589, Japan.
700    1_
$a Taneda, Akito $u 3​Graduate School of Science and Technology, Hirosaki University, Bunkyo-cho 3, Hirosaki 036-8561, Japan.
700    1_
$a He, Ying-Hong $u 1​Faculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, Hirosaki 036-8561, Japan.
700    1_
$a Tsushima, Taro $u 1​Faculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, Hirosaki 036-8561, Japan.
700    1_
$a Duraisamy, Ganesh Selvaraj $u 4​Biology Centre ASCR v.v.i, Institute of Plant Molecular Biology, Branišovská 31, České Budějovice 370 05, Czech Republic.
700    1_
$a Siglová, Kristyna $u 4​Biology Centre ASCR v.v.i, Institute of Plant Molecular Biology, Branišovská 31, České Budějovice 370 05, Czech Republic 5​University of South Bohemia, Faculty of Science, Branišovská 31, České Budějovice 370 05, Czech Republic.
700    1_
$a Matoušek, Jaroslav $u 4​Biology Centre ASCR v.v.i, Institute of Plant Molecular Biology, Branišovská 31, České Budějovice 370 05, Czech Republic.
700    1_
$a Sano, Teruo $u 1​Faculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, Hirosaki 036-8561, Japan.
773    0_
$w MED00002692 $t The Journal of general virology $x 1465-2099 $g Roč. 98, č. 3 (2017), s. 506-516
856    41
$u https://pubmed.ncbi.nlm.nih.gov/28005527 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20170720 $b ABA008
991    __
$a 20170831103657 $b ABA008
999    __
$a ok $b bmc $g 1239096 $s 984328
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2017 $b 98 $c 3 $d 506-516 $e 20170401 $i 1465-2099 $m Journal of general virology $n J Gen Virol $x MED00002692
LZP    __
$a Pubmed-20170720

Najít záznam

Citační ukazatele

Možnosti archivace