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Adipogenesis, lipogenesis and lipolysis is stimulated by mild but not severe hypoxia in 3T3-L1 cells

M. Weiszenstein, M. Musutova, A. Plihalova, K. Westlake, M. Elkalaf, M. Koc, A. Prochazka, J. Pala, S. Gulati, J. Trnka, J. Polak,

. 2016 ; 478 (2) : 727-32. [pub] 20160804

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc17023798

Grantová podpora
NV15-30155A MZ0 CEP - Centrální evidence projektů

In-vitro investigation of the effects of hypoxia is limited by physical laws of gas diffusion and cellular O2 consumption, making prolonged exposures to stable O2 concentrations impossible. Using a gas-permeable cultureware, chronic effects of mild and severe hypoxia on triglyceride accumulation, lipid droplet size distribution, spontaneous lipolysis and gene expression of adipocyte-specific markers were assessed. 3T3-L1 cells were differentiated under 20%, 4% or 1% O2 using a gas-permeable cultureware. Triglyceride accumulation, expression of genes characteristic for advanced adipocyte differentiation and involvement of key lipogenesis enzymes were assessed after exposures. Lipogenesis increased by 375% under mild hypoxia, but dropped by 43% in severe hypoxia. Mild, but not severe, hypoxia increased formation of large lipid droplets 6.4 fold and strongly induced gene expression of adipocyte-specific markers. Spontaneous lipolysis increased by 488% in mild, but only by 135% in severe hypoxia. Inhibition of ATP-dependent citrate lyase suppressed hypoxia-induced lipogenesis by 81% and 85%. Activation of HIF inhibited lipogenesis by 59%. Mild, but not severe, hypoxia stimulates lipolysis and promotes adipocyte differentiation, probably through excess of acetyl-CoA originating from tricarboxylic acid cycle independently of HIF activation.

Citace poskytuje Crossref.org

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$a Elkalaf, Moustafa $u Laboratory for Metabolism and Bioenergetics, Third Faculty of Medicine, Charles University in Prague, Ruska 87, Prague, Czech Republic.
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$a Pala, Jan $u Department of Sport Medicine, Third Faculty of Medicine, Charles University in Prague, Ruska 87, Prague, Czech Republic.
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