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Pokročilé metody studia vzájemných interakcí proteinů s DNA
[Advanced approaches in Study of DNA-protein interaction]
Lucie Béresová, René Lenobel
Language Czech Country Czech Republic
- MeSH
- Chromatography, Affinity * utilization MeSH
- DNA-Binding Proteins * analysis MeSH
- DNA MeSH
- Microscopy, Fluorescence * MeSH
- Mass Spectrometry * MeSH
- Electrophoretic Mobility Shift Assay * MeSH
- Protein Binding MeSH
DNA-protein interactions play an essential role in many regulatory mechanisms such as replication, transcription or translation and are responsible for the maintenance of the genome integrity. Isolation, identification and subsequent characterization of the biological function of DNA binding proteins propose insight into the pathological mechanisms that underlie various diseases. This review brings an overview of methods used for isolation and identification of DNA binding proteins (DNA-affinity chromatography coupled with quantitative proteomics and mass spectrometry) and subsequent methods for the characterization of their biological functions (fluorescence microscopy). Principles, advantages and disadvantages of individual methods are briefly discussed.
Advanced approaches in Study of DNA-protein interaction
Literatura
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- $a Béresová, Lucie $7 xx0329564 $u Ústav molekulární a translační medicíny, Lékařská fakulta, Univerzita Palackého v Olomouci, Olomouc; Centrum regionu Haná pro biotechnologický a zemědělský výzkum, Oddělení biochemie proteinů a proteomiky, Přírodovědecká fakulta, Univerzita Palackého v Olomouci, Olomouc
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- $a Pokročilé metody studia vzájemných interakcí proteinů s DNA / $c Lucie Béresová, René Lenobel
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- $a Advanced approaches in Study of DNA-protein interaction
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- $a Literatura
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- $a DNA-protein interactions play an essential role in many regulatory mechanisms such as replication, transcription or translation and are responsible for the maintenance of the genome integrity. Isolation, identification and subsequent characterization of the biological function of DNA binding proteins propose insight into the pathological mechanisms that underlie various diseases. This review brings an overview of methods used for isolation and identification of DNA binding proteins (DNA-affinity chromatography coupled with quantitative proteomics and mass spectrometry) and subsequent methods for the characterization of their biological functions (fluorescence microscopy). Principles, advantages and disadvantages of individual methods are briefly discussed.
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- $t Chemické listy $x 0009-2770 $g Roč. 111, č. 2 (2017), s. 136-141 $w MED00011010
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- $u http://www.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/33/33 $y domovská stránka časopisu
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