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Monoterpene alpha-terpinene induced hepatic oxidative, cytotoxic and genotoxic damage is associated to caspase activation in rats
Matheus D. Baldissera, Carine F. Souza, Geisa S. Dolci, Thirssa H. Grando, Michele R. Sagrillo, Rodrigo A. Vaucher, Sônia C.A. da Luz, Sérgio O. Silveira, Marta M.M.F. Duarte, Thiago Duarte, Aleksandro S. da Silva, Silvia G. Monteiro
Language English Country Czech Republic
- MeSH
- Apoptosis drug effects MeSH
- Glutathione Transferase drug effects MeSH
- Immunoassay MeSH
- Liver * pathology drug effects MeSH
- Caspases * MeSH
- Comet Assay MeSH
- Rats MeSH
- Monoterpenes * administration & dosage toxicity MeSH
- Oxidative Stress MeSH
- DNA Damage drug effects MeSH
- Rats, Wistar MeSH
- Reactive Oxygen Species analysis MeSH
- Mutagenicity Tests MeSH
- Cell Survival drug effects MeSH
- Dose-Response Relationship, Drug MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Animals MeSH
The aim of this study was to investigate the occurrence of toxic effects in liver tissue of rats treated with α-terpinene. All treatments were intraperitoneally administered at doses of 0.5, 0.75 and 1.0 ml kg−1 during 10 days. Liver samples were collected and assessed by histopathological analysis, caspases -1, -3, -8 assay, biomarkers of hepatic damage and determination of oxidant/antioxidant status (thiobarbituric acid-reactive substances (TBARS), catalase (CAT), superoxide dismutase (SOD), reactive oxygen species (ROS), glutathione S-transferase (GST) and glutathione peroxidase (GPx)). Additionally, the cytotoxic and genotoxic effects were evaluated by comet assay. An increase was observed on TBARS levels and GPx activity on the hepatic tissue. Instead, CAT and SOD activities decreased in rats treated with a dose of 1.0 ml kg−1 of α-terpinene. Concomitantly, ROS levels increased and GST levels decreased in rats treated with α-terpinene at doses of 0.5, 0.75 and 1.0 ml kg−1. Also, there was an increase in frequency of damage, damage index and caspases, while cell viability decreased in rats treated with α-terpinene. Alanine aminotransferase and aspartate aminotransferase increased in rats treated with 1.0 ml kg−1 of α-terpinene. Therefore, α-terpinene induces oxidative stress, cytotoxic and genotoxic effects in liver tissue involving the caspases activation.
Department of Animal Science Universidade do Estado de Santa Catarina Chapecó SC Brazil
Department of Biochemistry UFSM Santa Maria RS Brazil
Department of Physiology and Pharmacology UFSM Santa Maria RS Brazil
Laboratory of Cell Culture Centro Universitário Franciscano Santa Maria RS Brazil
References provided by Crossref.org
Literatura
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- $a The aim of this study was to investigate the occurrence of toxic effects in liver tissue of rats treated with α-terpinene. All treatments were intraperitoneally administered at doses of 0.5, 0.75 and 1.0 ml kg−1 during 10 days. Liver samples were collected and assessed by histopathological analysis, caspases -1, -3, -8 assay, biomarkers of hepatic damage and determination of oxidant/antioxidant status (thiobarbituric acid-reactive substances (TBARS), catalase (CAT), superoxide dismutase (SOD), reactive oxygen species (ROS), glutathione S-transferase (GST) and glutathione peroxidase (GPx)). Additionally, the cytotoxic and genotoxic effects were evaluated by comet assay. An increase was observed on TBARS levels and GPx activity on the hepatic tissue. Instead, CAT and SOD activities decreased in rats treated with a dose of 1.0 ml kg−1 of α-terpinene. Concomitantly, ROS levels increased and GST levels decreased in rats treated with α-terpinene at doses of 0.5, 0.75 and 1.0 ml kg−1. Also, there was an increase in frequency of damage, damage index and caspases, while cell viability decreased in rats treated with α-terpinene. Alanine aminotransferase and aspartate aminotransferase increased in rats treated with 1.0 ml kg−1 of α-terpinene. Therefore, α-terpinene induces oxidative stress, cytotoxic and genotoxic effects in liver tissue involving the caspases activation.
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