• Je něco špatně v tomto záznamu ?

Akt substrate of 160 kDa dephosphorylation rate is reduced in insulin-stimulated rat skeletal muscle after acute exercise

E. B. Arias, H. Wang, G. D. Cartee

. 2018 ; 67 (1) : 143-147. [pub] 20171110

Jazyk angličtina Země Česko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc18031092

Because greater Akt substrate of 160 kDa (AS160) phosphorylation has been reported in insulin-stimulated skeletal muscles without improved Akt activation several hours post-exercise, we hypothesized that prior exercise would result in attenuated AS160 dephosphorylation in insulin-stimulated rat skeletal muscle. Epitrochlearis muscles were isolated from rats that were sedentary (SED) or exercised 3 h earlier (3 h post-exercise; 3hPEX). Paired muscles were incubated with [(3)H]-2-deoxyglucose (2-DG) without insulin or with insulin. Lysates from other insulin-stimulated muscles from SED or 3hPEX rats were evaluated using AS160(Thr642) and AS160(Ser588) dephosphorylation assays. Prior exercise led to greater 2-DG uptake concomitant with greater AS160(Thr642) phosphorylation and a non-significant trend (P=0.087) for greater AS160(Ser588). Prior exercise also reduced AS160(Thr642) and AS160(Ser588) dephosphorylation rates. These results support the idea that attenuated AS160 dephosphorylation may favor greater AS160 phosphorylation post-exercise.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc18031092
003      
CZ-PrNML
005      
20180920151446.0
007      
ta
008      
180914s2018 xr d f 000 0|eng||
009      
AR
024    7_
$a 10.33549/physiolres.933591 $2 doi
035    __
$a (PubMed)29137480
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xr
100    1_
$a Arias, E. B. $u School of Kinesiology, University of Michigan, Ann Arbor, MI, USA
245    10
$a Akt substrate of 160 kDa dephosphorylation rate is reduced in insulin-stimulated rat skeletal muscle after acute exercise / $c E. B. Arias, H. Wang, G. D. Cartee
520    9_
$a Because greater Akt substrate of 160 kDa (AS160) phosphorylation has been reported in insulin-stimulated skeletal muscles without improved Akt activation several hours post-exercise, we hypothesized that prior exercise would result in attenuated AS160 dephosphorylation in insulin-stimulated rat skeletal muscle. Epitrochlearis muscles were isolated from rats that were sedentary (SED) or exercised 3 h earlier (3 h post-exercise; 3hPEX). Paired muscles were incubated with [(3)H]-2-deoxyglucose (2-DG) without insulin or with insulin. Lysates from other insulin-stimulated muscles from SED or 3hPEX rats were evaluated using AS160(Thr642) and AS160(Ser588) dephosphorylation assays. Prior exercise led to greater 2-DG uptake concomitant with greater AS160(Thr642) phosphorylation and a non-significant trend (P=0.087) for greater AS160(Ser588). Prior exercise also reduced AS160(Thr642) and AS160(Ser588) dephosphorylation rates. These results support the idea that attenuated AS160 dephosphorylation may favor greater AS160 phosphorylation post-exercise.
650    _2
$a zvířata $7 D000818
650    _2
$a proteiny aktivující GTPasu $x metabolismus $7 D020690
650    _2
$a inzulin $x metabolismus $x farmakologie $7 D007328
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a kosterní svaly $x metabolismus $7 D018482
650    _2
$a fosforylace $x účinky léků $x fyziologie $7 D010766
650    _2
$a kondiční příprava zvířat $x fyziologie $7 D010805
650    _2
$a protoonkogenní proteiny c-akt $x metabolismus $7 D051057
650    _2
$a krysa rodu Rattus $7 D051381
650    _2
$a potkani Wistar $7 D017208
655    _2
$a časopisecké články $7 D016428
700    1_
$a Wang, H. $u School of Kinesiology, University of Michigan, Ann Arbor, MI, USA
700    1_
$a Cartee, G. D. $u Muscle Biology Laboratory, School of Kinesiology, University of Michigan, Ann Arbor, MI, USA; Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, MI, USA; Institute of Gerontology, University of Michigan, Ann Arbor, MI, USA
773    0_
$w MED00003824 $t Physiological research $x 1802-9973 $g Roč. 67, č. 1 (2018), s. 143-147
856    41
$u https://pubmed.ncbi.nlm.nih.gov/29137480 $y Pubmed
910    __
$a ABA008 $b A 4120 $c 266 $y 4 $z 0
990    __
$a 20180914 $b ABA008
991    __
$a 20180919102421 $b ABA008
999    __
$a ok $b bmc $g 1335335 $s 1028065
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2018 $b 67 $c 1 $d 143-147 $e 20171110 $i 1802-9973 $m Physiological research $n Physiol. Res. (Print) $x MED00003824
LZP    __
$b NLK118 $a Pubmed-20180914

Najít záznam

Citační ukazatele

Možnosti archivace