• Je něco špatně v tomto záznamu ?

Cellular differentiation of non-transformed intestinal epithelial cells is regulated by Lactobacillus rhamnosus and L. casei strains

J. Kolinska, M. Zakostelecka, Z. Zemanova, V. Lisa, J. Golias, H. Kozakova, B. Dvorak

. 2018 ; 67 (2) : 261-273. [pub] 20180105

Jazyk angličtina Země Česko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc18036407

The aim of this study was to characterize an in vitro modulating effect of three commensal Lactobacillus strains on cellular differentiation of non-transformed crypt-like rat small intestinal cell line IEC-18. IEC-18 was grown on extracellular matrix, with or without presence of Lactobacillus strains. Gene expression of IEC-18 bacterial detection system - such as Toll-like receptors TLR-2, TLR-4, signal adapter MyD88, cytoplasmic NOD2 receptor, inflammatory cytokines IL-18, IL-1beta, chemokine IL-8 and enzyme caspase-1 - was evaluated using real-time PCR. Expression and localization of TLR-2, TLR-4, IL-18 and caspase-1 proteins was demonstrated by Western blotting and immunofluorescent staining. Secretion of IL-18 to apical and basolateral surfaces was assayed by ELISA. Our results suggested that L. casei LOCK0919 accelerated differentiation of IEC-18 by stimulating TLR-2, TLR-4, MyD88, IL-18, caspase-1 mRNAs and proteins. L. casei LOCK0919 increased expression and transfer of villin and beta-catenin from cytoplasm to cell membrane. Presence of L. rhamnosus LOCK0900 resulted in detachment of IEC-18 layer from extracellular matrix leading to induction of IL-1beta, of TLR-2 and IL-8 mRNAs and stimulation of MyD88, caspase-1 and cytosolic receptor NOD2 mRNAs. L. rhamnosus LOCK0908 was not recognized by TLR-2 or TLR-4 receptors. Lactobacilli-IEC-18 crosstalk enhanced immune and barrier mucosal functions.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc18036407
003      
CZ-PrNML
005      
20181102123637.0
007      
ta
008      
181030s2018 xr ad f 000 0|eng||
009      
AR
024    7_
$a 10.33549/physiolres.933643 $2 doi
035    __
$a (PubMed)29303613
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xr
100    1_
$a Kolínská, Jiřina $u Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic $7 xx0106974
245    10
$a Cellular differentiation of non-transformed intestinal epithelial cells is regulated by Lactobacillus rhamnosus and L. casei strains / $c J. Kolinska, M. Zakostelecka, Z. Zemanova, V. Lisa, J. Golias, H. Kozakova, B. Dvorak
520    9_
$a The aim of this study was to characterize an in vitro modulating effect of three commensal Lactobacillus strains on cellular differentiation of non-transformed crypt-like rat small intestinal cell line IEC-18. IEC-18 was grown on extracellular matrix, with or without presence of Lactobacillus strains. Gene expression of IEC-18 bacterial detection system - such as Toll-like receptors TLR-2, TLR-4, signal adapter MyD88, cytoplasmic NOD2 receptor, inflammatory cytokines IL-18, IL-1beta, chemokine IL-8 and enzyme caspase-1 - was evaluated using real-time PCR. Expression and localization of TLR-2, TLR-4, IL-18 and caspase-1 proteins was demonstrated by Western blotting and immunofluorescent staining. Secretion of IL-18 to apical and basolateral surfaces was assayed by ELISA. Our results suggested that L. casei LOCK0919 accelerated differentiation of IEC-18 by stimulating TLR-2, TLR-4, MyD88, IL-18, caspase-1 mRNAs and proteins. L. casei LOCK0919 increased expression and transfer of villin and beta-catenin from cytoplasm to cell membrane. Presence of L. rhamnosus LOCK0900 resulted in detachment of IEC-18 layer from extracellular matrix leading to induction of IL-1beta, of TLR-2 and IL-8 mRNAs and stimulation of MyD88, caspase-1 and cytosolic receptor NOD2 mRNAs. L. rhamnosus LOCK0908 was not recognized by TLR-2 or TLR-4 receptors. Lactobacilli-IEC-18 crosstalk enhanced immune and barrier mucosal functions.
650    _2
$a zvířata $7 D000818
650    _2
$a kaspasa 1 $x biosyntéza $7 D020170
650    _2
$a buněčná diferenciace $x účinky léků $7 D002454
650    _2
$a cytokiny $x biosyntéza $7 D016207
650    _2
$a epitelové buňky $x účinky léků $7 D004847
650    _2
$a regulace genové exprese $x účinky léků $7 D005786
650    _2
$a interleukin-18 $x biosyntéza $7 D020382
650    _2
$a střevní sliznice $x cytologie $x účinky léků $7 D007413
650    12
$a Lactobacillus casei $7 D007780
650    12
$a Lacticaseibacillus rhamnosus $7 D052201
650    _2
$a mikrofilamentové proteiny $x biosyntéza $7 D008840
650    _2
$a probiotika $x farmakologie $7 D019936
650    _2
$a messenger RNA $x biosyntéza $7 D012333
650    _2
$a krysa rodu Rattus $7 D051381
650    _2
$a subcelulární frakce $x metabolismus $7 D013347
650    _2
$a toll-like receptory $x biosyntéza $x účinky léků $7 D051193
650    _2
$a beta-katenin $x biosyntéza $7 D051176
655    _2
$a časopisecké články $7 D016428
700    1_
$a Zákostelecká, Marie $7 xx0229135 $u Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic
700    1_
$a Zemanová, Zdeňka $7 xx0074462 $u Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic
700    1_
$a Lisá, Věra $7 xx0063962 $u Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic
700    1_
$a Goliáš, Jiří $7 _AN098218 $u Laboratory of Cellular and Molecular Immunology, Institute of Microbiology of the Czech Academy of Sciences, Prague, Czech Republic
700    1_
$a Kozáková, Hana, $d 1952- $7 xx0153144 $u Laboratory of Gnotobiology, Institute of Microbiology of the Czech Academy of Sciences, Nový Hrádek, Czech Republic
700    1_
$a Dvořák, Bohuslav $7 xx0229194 $u Department of Pediatrics and the Steele Children's Research Center, The University of Arizona, Tucson, AZ, USA
773    0_
$w MED00003824 $t Physiological research $x 1802-9973 $g Roč. 67, č. 2 (2018), s. 261-273
856    41
$u https://pubmed.ncbi.nlm.nih.gov/29303613 $y Pubmed
910    __
$a ABA008 $b A 4120 $c 266 $y 4 $z 0
990    __
$a 20181030 $b ABA008
991    __
$a 20181102095418 $b ABA008
999    __
$a ok $b bmc $g 1347830 $s 1033431
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2018 $b 67 $c 2 $d 261-273 $e 20180105 $i 1802-9973 $m Physiological research $n Physiol. Res. (Print) $x MED00003824
LZP    __
$b NLK118 $a Pubmed-20181030

Najít záznam

Citační ukazatele

Možnosti archivace