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Monocyte derived macrophages as an appropriate model for porcine cytomegalovirus immunobiology studies
L. Kavanová, R. Moutelíková, J. Prodělalová, M. Faldyna, M. Toman, J. Salát,
Jazyk angličtina Země Nizozemsko
Typ dokumentu časopisecké články
- MeSH
- cytokiny imunologie MeSH
- cytomegalovirové infekce imunologie veterinární MeSH
- Cytomegalovirus fyziologie MeSH
- exprese genu imunologie MeSH
- interleukin-10 imunologie MeSH
- interleukin-8 imunologie MeSH
- makrofágy imunologie virologie MeSH
- nemoci prasat imunologie virologie MeSH
- prasata MeSH
- přirozená imunita MeSH
- TNF-alfa imunologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Porcine cytomegalovirus (PCMV) causes lifelong latent infections in swine. The pathogen is occasionally associated with inclusion body rhinitis and pneumonia in piglets, reproductive disorders in pregnant sows and respiratory disease complex in older pigs. Immunosuppressive potential of PCMV infection is discussed. Macrophages were recognised as one of target cell types where propagation of virus occurs. The aim of present study was to set up model PCMV infection of monocyte derived macrophages (MDMs) in vitro for PCMV immunobiology research. Obtained results showed that PCMV is able to infect and propagate in MDMs. Possible immunosuppressive effect of PCMV on infected macrophages was evaluated by measurement of immune relevant gene expression in MDMs. Infection decreased expression of IL-8 and TNF-α (pro-inflammatory cytokines) and increased expression of IL-10 (anti-inflammatory cytokine) on mRNA transcription level. Obtained data support hypothesis that higher sensitivity of animals to coinfection with other swine pathogens and its more severe clinical manifestations could potentially be the consequence of PCMV infection.
Citace poskytuje Crossref.org
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