-
Something wrong with this record ?
The Unique Mechanisms of Cellular Proliferation, Migration and Apoptosis are Regulated through Oocyte Maturational Development-A Complete Transcriptomic and Histochemical Study
B. Chermuła, M. Brązert, M. Jeseta, K. Ożegowska, P. Sujka-Kordowska, A. Konwerska, A. Bryja, W. Kranc, M. Jankowski, MJ. Nawrocki, I. Kocherova, P. Celichowski, B. Borowiec, M. Popis, J. Budna-Tukan, P. Antosik, D. Bukowska, KP. Brussow, L....
Language English Country Switzerland
Document type Journal Article
Grant support
UMO-2016/21/B/NZ9/03535
Narodowe Centrum Nauki
NLK
Free Medical Journals
from 2000
Freely Accessible Science Journals
from 2000
PubMed Central
from 2007
Europe PubMed Central
from 2007
ProQuest Central
from 2000-03-01
Open Access Digital Library
from 2000-01-01
Open Access Digital Library
from 2007-01-01
Health & Medicine (ProQuest)
from 2000-03-01
ROAD: Directory of Open Access Scholarly Resources
from 2000
PubMed
30587792
DOI
10.3390/ijms20010084
Knihovny.cz E-resources
- MeSH
- Apoptosis genetics MeSH
- Down-Regulation MeSH
- Gene Regulatory Networks MeSH
- In Vitro Oocyte Maturation Techniques MeSH
- Cumulus Cells metabolism pathology MeSH
- Oocytes growth & development metabolism pathology MeSH
- Cell Movement genetics MeSH
- Swine MeSH
- Cell Proliferation genetics MeSH
- RNA genetics metabolism MeSH
- Oligonucleotide Array Sequence Analysis MeSH
- Gene Expression Profiling MeSH
- Transcriptome * MeSH
- Up-Regulation MeSH
- Animals MeSH
- Check Tag
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
The growth and development of oocyte affect the functional activities of the surrounding somatic cells. These cells are regulated by various types of hormones, proteins, metabolites, and regulatory molecules through gap communication, ultimately leading to the development and maturation of oocytes. The close association between somatic cells and oocytes, which together form the cumulus-oocyte complexes (COCs), and their bi-directional communication are crucial for the acquisition of developmental competences by the oocyte. In this study, oocytes were extracted from the ovaries obtained from crossbred landrace gilts and subjected to in vitro maturation. RNA isolated from those oocytes was used for the subsequent microarray analysis. The data obtained shows, for the first time, variable levels of gene expression (fold changes higher than |2| and adjusted p-value < 0.05) belonging to four ontological groups: regulation of cell proliferation (GO:0042127), regulation of cell migration (GO:0030334), and regulation of programmed cell death (GO:0043067) that can be used together as proliferation, migration or apoptosis markers. We have identified several genes of porcine oocytes (ID2, VEGFA, BTG2, ESR1, CCND2, EDNRA, ANGPTL4, TGFBR3, GJA1, LAMA2, KIT, TPM1, VCP, GRID2, MEF2C, RPS3A, PLD1, BTG3, CD47, MITF), whose expression after in vitro maturation (IVM) is downregulated with different degrees. Our results may be helpful in further elucidating the molecular basis and functional significance of a number of gene markers associated with the processes of migration, proliferation and angiogenesis occurring in COCs.
Department of Anatomy Poznan University of Medical Sciences 60 781 Poznań Poland
Department of Histology and Embryology Poznan University of Medical Sciences 60 781 Poznań Poland
Veterinary Center Nicolaus Copernicus University in Torun 87 100 Toruń Poland
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc19027957
- 003
- CZ-PrNML
- 005
- 20190815114638.0
- 007
- ta
- 008
- 190813s2018 sz f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.3390/ijms20010084 $2 doi
- 035 __
- $a (PubMed)30587792
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a sz
- 100 1_
- $a Chermuła, Błażej $u Division of Infertility and Reproductive Endocrinology, Department of Gynecology, Obstetrics and Gynecological Oncology, Poznan University of Medical Sciences, 60-535 Poznań, Poland. blazej.chermula@wp.pl.
- 245 14
- $a The Unique Mechanisms of Cellular Proliferation, Migration and Apoptosis are Regulated through Oocyte Maturational Development-A Complete Transcriptomic and Histochemical Study / $c B. Chermuła, M. Brązert, M. Jeseta, K. Ożegowska, P. Sujka-Kordowska, A. Konwerska, A. Bryja, W. Kranc, M. Jankowski, MJ. Nawrocki, I. Kocherova, P. Celichowski, B. Borowiec, M. Popis, J. Budna-Tukan, P. Antosik, D. Bukowska, KP. Brussow, L. Pawelczyk, M. Bruska, M. Zabel, M. Nowicki, B. Kempisty,
- 520 9_
- $a The growth and development of oocyte affect the functional activities of the surrounding somatic cells. These cells are regulated by various types of hormones, proteins, metabolites, and regulatory molecules through gap communication, ultimately leading to the development and maturation of oocytes. The close association between somatic cells and oocytes, which together form the cumulus-oocyte complexes (COCs), and their bi-directional communication are crucial for the acquisition of developmental competences by the oocyte. In this study, oocytes were extracted from the ovaries obtained from crossbred landrace gilts and subjected to in vitro maturation. RNA isolated from those oocytes was used for the subsequent microarray analysis. The data obtained shows, for the first time, variable levels of gene expression (fold changes higher than |2| and adjusted p-value < 0.05) belonging to four ontological groups: regulation of cell proliferation (GO:0042127), regulation of cell migration (GO:0030334), and regulation of programmed cell death (GO:0043067) that can be used together as proliferation, migration or apoptosis markers. We have identified several genes of porcine oocytes (ID2, VEGFA, BTG2, ESR1, CCND2, EDNRA, ANGPTL4, TGFBR3, GJA1, LAMA2, KIT, TPM1, VCP, GRID2, MEF2C, RPS3A, PLD1, BTG3, CD47, MITF), whose expression after in vitro maturation (IVM) is downregulated with different degrees. Our results may be helpful in further elucidating the molecular basis and functional significance of a number of gene markers associated with the processes of migration, proliferation and angiogenesis occurring in COCs.
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a apoptóza $x genetika $7 D017209
- 650 _2
- $a pohyb buněk $x genetika $7 D002465
- 650 _2
- $a proliferace buněk $x genetika $7 D049109
- 650 _2
- $a kumulární buňky $x metabolismus $x patologie $7 D054885
- 650 _2
- $a down regulace $7 D015536
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a stanovení celkové genové exprese $7 D020869
- 650 _2
- $a genové regulační sítě $7 D053263
- 650 _2
- $a IVM techniky $7 D059471
- 650 _2
- $a sekvenční analýza hybridizací s uspořádaným souborem oligonukleotidů $7 D020411
- 650 _2
- $a oocyty $x růst a vývoj $x metabolismus $x patologie $7 D009865
- 650 _2
- $a RNA $x genetika $x metabolismus $7 D012313
- 650 _2
- $a prasata $7 D013552
- 650 12
- $a transkriptom $7 D059467
- 650 _2
- $a upregulace $7 D015854
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Brązert, Maciej $u Division of Infertility and Reproductive Endocrinology, Department of Gynecology, Obstetrics and Gynecological Oncology, Poznan University of Medical Sciences, 60-535 Poznań, Poland. maciejbrazert@ump.edu.pl.
- 700 1_
- $a Jeseta, Michal $u Department of Obstetrics and Gynecology, University Hospital and Masaryk University, 601 77 Brno, Czech Republic. jeseta@gmail.com.
- 700 1_
- $a Ożegowska, Katarzyna $u Division of Infertility and Reproductive Endocrinology, Department of Gynecology, Obstetrics and Gynecological Oncology, Poznan University of Medical Sciences, 60-535 Poznań, Poland. katarzyna.ozegowska@ump.edu.pl.
- 700 1_
- $a Sujka-Kordowska, Patrycja $u Department of Histology and Embryology, Poznan University of Medical Sciences, 60-781 Poznań, Poland. psujka@ump.edu.pl.
- 700 1_
- $a Konwerska, Aneta $u Department of Histology and Embryology, Poznan University of Medical Sciences, 60-781 Poznań, Poland. akonwer@ump.edu.pl.
- 700 1_
- $a Bryja, Artur $u Department of Anatomy, Poznan University of Medical Sciences, 60-781 Poznań, Poland. abryja@ump.edu.pl.
- 700 1_
- $a Kranc, Wiesława $u Department of Anatomy, Poznan University of Medical Sciences, 60-781 Poznań, Poland. wkranc@ump.edu.pl.
- 700 1_
- $a Jankowski, Maurycy $u Department of Anatomy, Poznan University of Medical Sciences, 60-781 Poznań, Poland. m.jankowski.14@aberdeen.ac.uk.
- 700 1_
- $a Nawrocki, Mariusz J $u Department of Anatomy, Poznan University of Medical Sciences, 60-781 Poznań, Poland. mjnawrocki@ump.edu.pl.
- 700 1_
- $a Kocherova, Ievgeniia $u Department of Anatomy, Poznan University of Medical Sciences, 60-781 Poznań, Poland. ikocherova@ump.edu.pl.
- 700 1_
- $a Celichowski, Piotr $u Department of Histology and Embryology, Poznan University of Medical Sciences, 60-781 Poznań, Poland. pcelichowski@ump.edu.pl.
- 700 1_
- $a Borowiec, Blanka $u Department of Anatomy, Poznan University of Medical Sciences, 60-781 Poznań, Poland. blanka.maria.b@gmail.com.
- 700 1_
- $a Popis, Małgorzata $u Department of Anatomy, Poznan University of Medical Sciences, 60-781 Poznań, Poland. malgorzatapopis111@gmail.com.
- 700 1_
- $a Budna-Tukan, Joanna $u Department of Histology and Embryology, Poznan University of Medical Sciences, 60-781 Poznań, Poland. jbudna@ump.edu.pl.
- 700 1_
- $a Antosik, Paweł $u Veterinary Center, Nicolaus Copernicus University in Torun, 87-100 Toruń, Poland. pantosik@umk.pl.
- 700 1_
- $a Bukowska, Dorota $u Veterinary Center, Nicolaus Copernicus University in Torun, 87-100 Toruń, Poland. dbukowska@umk.pl.
- 700 1_
- $a Brussow, Klaus P $u Veterinary Center, Nicolaus Copernicus University in Torun, 87-100 Toruń, Poland. prof.bruessow@gmail.com.
- 700 1_
- $a Pawelczyk, Leszek $u Division of Infertility and Reproductive Endocrinology, Department of Gynecology, Obstetrics and Gynecological Oncology, Poznan University of Medical Sciences, 60-535 Poznań, Poland. pawelczyk.leszek@ump.edu.pl.
- 700 1_
- $a Bruska, Małgorzata $u Department of Anatomy, Poznan University of Medical Sciences, 60-781 Poznań, Poland. mbruska@ump.edu.pl.
- 700 1_
- $a Zabel, Maciej $u Department of Histology and Embryology, Wroclaw University of Medical Sciences, 50-368 Wrocław, Poland. mazab@ump.edu.pl. Division of Anatomy and Histology, University of Zielona Gora, 65-046 Zielona Góra, Poland. mazab@ump.edu.pl.
- 700 1_
- $a Nowicki, Michał $u Department of Histology and Embryology, Poznan University of Medical Sciences, 60-781 Poznań, Poland. mnowicki@ump.edu.pl.
- 700 1_
- $a Kempisty, Bartosz $u Department of Obstetrics and Gynecology, University Hospital and Masaryk University, 601 77 Brno, Czech Republic. bkempisty@ump.edu.pl. Department of Histology and Embryology, Poznan University of Medical Sciences, 60-781 Poznań, Poland. bkempisty@ump.edu.pl. Department of Anatomy, Poznan University of Medical Sciences, 60-781 Poznań, Poland. bkempisty@ump.edu.pl.
- 773 0_
- $w MED00176142 $t International journal of molecular sciences $x 1422-0067 $g Roč. 20, č. 1 (2018)
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/30587792 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20190813 $b ABA008
- 991 __
- $a 20190815114906 $b ABA008
- 999 __
- $a ok $b bmc $g 1433106 $s 1066417
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2018 $b 20 $c 1 $e 20181226 $i 1422-0067 $m International journal of molecular sciences $n Int J Mol Sci $x MED00176142
- GRA __
- $a UMO-2016/21/B/NZ9/03535 $p Narodowe Centrum Nauki
- LZP __
- $a Pubmed-20190813
