Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

iTAP, a novel iRhom interactor, controls TNF secretion by policing the stability of iRhom/TACE

I. Oikonomidi, E. Burbridge, M. Cavadas, G. Sullivan, B. Collis, H. Naegele, D. Clancy, J. Brezinova, T. Hu, A. Bileck, C. Gerner, A. Bolado, A. von Kriegsheim, SJ. Martin, F. Steinberg, K. Strisovsky, C. Adrain,

. 2018 ; 7 (-) : . [pub] 20180613

Language English Country England, Great Britain

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc19028380

Grant support
14/IA/2622 Science Foundation Ireland - Ireland
14-1289 Worldwide Cancer Research - United Kingdom

The apical inflammatory cytokine TNF regulates numerous important biological processes including inflammation and cell death, and drives inflammatory diseases. TNF secretion requires TACE (also called ADAM17), which cleaves TNF from its transmembrane tether. The trafficking of TACE to the cell surface, and stimulation of its proteolytic activity, depends on membrane proteins, called iRhoms. To delineate how the TNF/TACE/iRhom axis is regulated, we performed an immunoprecipitation/mass spectrometry screen to identify iRhom-binding proteins. This identified a novel protein, that we name iTAP (iRhom Tail-Associated Protein) that binds to iRhoms, enhancing the cell surface stability of iRhoms and TACE, preventing their degradation in lysosomes. Depleting iTAP in primary human macrophages profoundly impaired TNF production and tissues from iTAP KO mice exhibit a pronounced depletion in active TACE levels. Our work identifies iTAP as a physiological regulator of TNF signalling and a novel target for the control of inflammation.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc19028380
003      
CZ-PrNML
005      
20190819121001.0
007      
ta
008      
190813s2018 enk f 000 0|eng||
009      
AR
024    7_
$a 10.7554/eLife.35032 $2 doi
035    __
$a (PubMed)29897333
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Oikonomidi, Ioanna $u Membrane Traffic Lab, Instituto Gulbenkian de Ciência, Oeiras, Portugal.
245    10
$a iTAP, a novel iRhom interactor, controls TNF secretion by policing the stability of iRhom/TACE / $c I. Oikonomidi, E. Burbridge, M. Cavadas, G. Sullivan, B. Collis, H. Naegele, D. Clancy, J. Brezinova, T. Hu, A. Bileck, C. Gerner, A. Bolado, A. von Kriegsheim, SJ. Martin, F. Steinberg, K. Strisovsky, C. Adrain,
520    9_
$a The apical inflammatory cytokine TNF regulates numerous important biological processes including inflammation and cell death, and drives inflammatory diseases. TNF secretion requires TACE (also called ADAM17), which cleaves TNF from its transmembrane tether. The trafficking of TACE to the cell surface, and stimulation of its proteolytic activity, depends on membrane proteins, called iRhoms. To delineate how the TNF/TACE/iRhom axis is regulated, we performed an immunoprecipitation/mass spectrometry screen to identify iRhom-binding proteins. This identified a novel protein, that we name iTAP (iRhom Tail-Associated Protein) that binds to iRhoms, enhancing the cell surface stability of iRhoms and TACE, preventing their degradation in lysosomes. Depleting iTAP in primary human macrophages profoundly impaired TNF production and tissues from iTAP KO mice exhibit a pronounced depletion in active TACE levels. Our work identifies iTAP as a physiological regulator of TNF signalling and a novel target for the control of inflammation.
650    _2
$a protein ADAM17 $x genetika $x metabolismus $7 D000072198
650    _2
$a sekvence aminokyselin $7 D000595
650    _2
$a zvířata $7 D000818
650    _2
$a transportní proteiny $x genetika $x metabolismus $7 D002352
650    _2
$a buněčné linie $7 D002460
650    _2
$a cytoskeletální proteiny $x genetika $x metabolismus $7 D003598
650    _2
$a endozomy $x metabolismus $7 D011992
650    _2
$a fibroblasty $x cytologie $x metabolismus $7 D005347
650    _2
$a regulace genové exprese $7 D005786
650    _2
$a HEK293 buňky $7 D057809
650    _2
$a HeLa buňky $7 D006367
650    _2
$a lidé $7 D006801
650    _2
$a makrofágy $x cytologie $x metabolismus $7 D008264
650    _2
$a membránové proteiny $x genetika $x metabolismus $7 D008565
650    _2
$a myši $7 D051379
650    _2
$a myši inbrední C57BL $7 D008810
650    _2
$a myši knockoutované $7 D018345
650    _2
$a primární buněčná kultura $7 D061251
650    _2
$a vazba proteinů $7 D011485
650    _2
$a proteolýza $7 D059748
650    _2
$a RAW 264.7 buňky $7 D000067996
650    _2
$a sekvenční seřazení $7 D016415
650    _2
$a sekvenční homologie aminokyselin $7 D017386
650    _2
$a signální transdukce $7 D015398
650    _2
$a TNF-alfa $x genetika $x metabolismus $7 D014409
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Burbridge, Emma $u Membrane Traffic Lab, Instituto Gulbenkian de Ciência, Oeiras, Portugal.
700    1_
$a Cavadas, Miguel $u Membrane Traffic Lab, Instituto Gulbenkian de Ciência, Oeiras, Portugal.
700    1_
$a Sullivan, Graeme $u Molecular Cell Biology Laboratory, Department of Genetics, The Smurfit Institute, Trinity College Dublin, Dublin, Ireland.
700    1_
$a Collis, Blanka $u Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
700    1_
$a Naegele, Heike $u Center for Biological Systems Analysis, Faculty of Biology, Albert Ludwigs Universitaet Freiburg, Freiburg, Germany.
700    1_
$a Clancy, Danielle $u Molecular Cell Biology Laboratory, Department of Genetics, The Smurfit Institute, Trinity College Dublin, Dublin, Ireland.
700    1_
$a Brezinova, Jana $u Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
700    1_
$a Hu, Tianyi $u Membrane Traffic Lab, Instituto Gulbenkian de Ciência, Oeiras, Portugal.
700    1_
$a Bileck, Andrea $u Institut für Analytische Chemie, Universität Wien, Vienna, Austria.
700    1_
$a Gerner, Christopher $u Institut für Analytische Chemie, Universität Wien, Vienna, Austria.
700    1_
$a Bolado, Alfonso $u Edinburgh Cancer Research UK Centre, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh, United Kingdom.
700    1_
$a von Kriegsheim, Alex $u Edinburgh Cancer Research UK Centre, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh, United Kingdom.
700    1_
$a Martin, Seamus J $u Molecular Cell Biology Laboratory, Department of Genetics, The Smurfit Institute, Trinity College Dublin, Dublin, Ireland.
700    1_
$a Steinberg, Florian $u Center for Biological Systems Analysis, Faculty of Biology, Albert Ludwigs Universitaet Freiburg, Freiburg, Germany.
700    1_
$a Strisovsky, Kvido $u Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
700    1_
$a Adrain, Colin $u Membrane Traffic Lab, Instituto Gulbenkian de Ciência, Oeiras, Portugal.
773    0_
$w MED00188753 $t eLife $x 2050-084X $g Roč. 7, č. - (2018)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/29897333 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20190813 $b ABA008
991    __
$a 20190819121235 $b ABA008
999    __
$a ok $b bmc $g 1433529 $s 1066840
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2018 $b 7 $c - $e 20180613 $i 2050-084X $m eLife $n eLife $x MED00188753
GRA    __
$a 14/IA/2622 $p Science Foundation Ireland $2 Ireland
GRA    __
$a 14-1289 $p Worldwide Cancer Research $2 United Kingdom
LZP    __
$a Pubmed-20190813

Find record

Citation metrics

Archiving options