• Something wrong with this record ?

The SMC5/6 Complex Subunit NSE4A Is Involved in DNA Damage Repair and Seed Development

M. Díaz, P. Pečinková, A. Nowicka, C. Baroux, T. Sakamoto, PY. Gandha, H. Jeřábková, S. Matsunaga, U. Grossniklaus, A. Pecinka,

. 2019 ; 31 (7) : 1579-1597. [pub] 20190429

Language English Country United States

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc20023870

The maintenance of genome integrity over cell divisions is critical for plant development and the correct transmission of genetic information to the progeny. A key factor involved in this process is the STRUCTURAL MAINTENANCE OF CHROMOSOME5 (SMC5) and SMC6 (SMC5/6) complex, related to the cohesin and condensin complexes that control sister chromatid alignment and chromosome condensation, respectively. Here, we characterize NON-SMC ELEMENT4 (NSE4) paralogs of the SMC5/6 complex in Arabidopsis (Arabidopsis thaliana). NSE4A is expressed in meristems and accumulates during DNA damage repair. Partial loss-of-function nse4a mutants are viable but hypersensitive to DNA damage induced by zebularine. In addition, nse4a mutants produce abnormal seeds, with noncellularized endosperm and embryos that maximally develop to the heart or torpedo stage. This phenotype resembles the defects in cohesin and condensin mutants and suggests a role for all three SMC complexes in differentiation during seed development. By contrast, NSE4B is expressed in only a few cell types, and loss-of-function mutants do not have any obvious abnormal phenotype. In summary, our study shows that the NSE4A subunit of the SMC5-SMC6 complex is essential for DNA damage repair in somatic tissues and plays a role in plant reproduction.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc20023870
003      
CZ-PrNML
005      
20201214131517.0
007      
ta
008      
201125s2019 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1105/tpc.18.00043 $2 doi
035    __
$a (PubMed)31036599
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Díaz, Mariana $u Department of Plant Breeding and Genetics, Max Planck Institute for Plant Breeding Research (MPIPZ), 50829 Cologne, Germany. The Czech Academy of Sciences, Institute of Experimental Botany (IEB), Centre of the Region Haná for Biotechnological and Agricultural Research, 77900 Olomouc, Czech Republic. Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Chiba 278-8510, Japan.
245    14
$a The SMC5/6 Complex Subunit NSE4A Is Involved in DNA Damage Repair and Seed Development / $c M. Díaz, P. Pečinková, A. Nowicka, C. Baroux, T. Sakamoto, PY. Gandha, H. Jeřábková, S. Matsunaga, U. Grossniklaus, A. Pecinka,
520    9_
$a The maintenance of genome integrity over cell divisions is critical for plant development and the correct transmission of genetic information to the progeny. A key factor involved in this process is the STRUCTURAL MAINTENANCE OF CHROMOSOME5 (SMC5) and SMC6 (SMC5/6) complex, related to the cohesin and condensin complexes that control sister chromatid alignment and chromosome condensation, respectively. Here, we characterize NON-SMC ELEMENT4 (NSE4) paralogs of the SMC5/6 complex in Arabidopsis (Arabidopsis thaliana). NSE4A is expressed in meristems and accumulates during DNA damage repair. Partial loss-of-function nse4a mutants are viable but hypersensitive to DNA damage induced by zebularine. In addition, nse4a mutants produce abnormal seeds, with noncellularized endosperm and embryos that maximally develop to the heart or torpedo stage. This phenotype resembles the defects in cohesin and condensin mutants and suggests a role for all three SMC complexes in differentiation during seed development. By contrast, NSE4B is expressed in only a few cell types, and loss-of-function mutants do not have any obvious abnormal phenotype. In summary, our study shows that the NSE4A subunit of the SMC5-SMC6 complex is essential for DNA damage repair in somatic tissues and plays a role in plant reproduction.
650    _2
$a Arabidopsis $x embryologie $x genetika $x imunologie $7 D017360
650    _2
$a proteiny huseníčku $x genetika $x metabolismus $7 D029681
650    _2
$a proteiny buněčného cyklu $x genetika $x metabolismus $7 D018797
650    12
$a poškození DNA $x genetika $7 D004249
650    12
$a oprava DNA $x genetika $7 D004260
650    _2
$a duplikace genu $7 D020440
650    _2
$a vývojová regulace genové exprese $7 D018507
650    _2
$a regulace genové exprese u rostlin $7 D018506
650    _2
$a genom rostlinný $7 D018745
650    _2
$a vajíčko rostlin $x genetika $7 D056771
650    _2
$a pyl $x genetika $7 D011058
650    _2
$a vazba proteinů $7 D011485
650    _2
$a podjednotky proteinů $x metabolismus $7 D021122
650    _2
$a semena rostlinná $x genetika $x metabolismus $7 D012639
650    _2
$a transkriptom $x genetika $7 D059467
650    _2
$a upregulace $x genetika $7 D015854
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Pečinková, Petra $u Department of Plant Breeding and Genetics, Max Planck Institute for Plant Breeding Research (MPIPZ), 50829 Cologne, Germany.
700    1_
$a Nowicka, Anna $u Department of Plant Breeding and Genetics, Max Planck Institute for Plant Breeding Research (MPIPZ), 50829 Cologne, Germany. The Czech Academy of Sciences, Institute of Experimental Botany (IEB), Centre of the Region Haná for Biotechnological and Agricultural Research, 77900 Olomouc, Czech Republic. Polish Academy of Sciences, Franciszek Gorski Institute of Plant Physiology, 30-239 Krakow, Poland.
700    1_
$a Baroux, Célia $u Department of Plant and Microbial Biology and Zürich-Basel Plant Science Center, University of Zürich, 8008 Zürich, Switzerland.
700    1_
$a Sakamoto, Takuya $u Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Chiba 278-8510, Japan.
700    1_
$a Gandha, Priscilla Yuliani $u Department of Plant Breeding and Genetics, Max Planck Institute for Plant Breeding Research (MPIPZ), 50829 Cologne, Germany.
700    1_
$a Jeřábková, Hana $u The Czech Academy of Sciences, Institute of Experimental Botany (IEB), Centre of the Region Haná for Biotechnological and Agricultural Research, 77900 Olomouc, Czech Republic.
700    1_
$a Matsunaga, Sachihiro $u Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Chiba 278-8510, Japan.
700    1_
$a Grossniklaus, Ueli $u Department of Plant and Microbial Biology and Zürich-Basel Plant Science Center, University of Zürich, 8008 Zürich, Switzerland.
700    1_
$a Pecinka, Ales $u Department of Plant Breeding and Genetics, Max Planck Institute for Plant Breeding Research (MPIPZ), 50829 Cologne, Germany pecinka@ueb.cas.cz. The Czech Academy of Sciences, Institute of Experimental Botany (IEB), Centre of the Region Haná for Biotechnological and Agricultural Research, 77900 Olomouc, Czech Republic.
773    0_
$w MED00005315 $t The Plant cell $x 1532-298X $g Roč. 31, č. 7 (2019), s. 1579-1597
856    41
$u https://pubmed.ncbi.nlm.nih.gov/31036599 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20201125 $b ABA008
991    __
$a 20201214131515 $b ABA008
999    __
$a ok $b bmc $g 1596189 $s 1114546
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2019 $b 31 $c 7 $d 1579-1597 $e 20190429 $i 1532-298X $m The Plant cell $n Plant Cell $x MED00005315
LZP    __
$a Pubmed-20201125

Find record

Citation metrics

Archiving options