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The effect of polyclonal activators on rabbit B cell antibody production
H. Stepanova, K. Hlavova, R. Machat, E. Jeklova, M. Faldyna,
Jazyk angličtina Země Velká Británie
Typ dokumentu časopisecké články
- MeSH
- alfa-makroglobuliny aplikace a dávkování imunologie MeSH
- B-lymfocyty imunologie MeSH
- králíci MeSH
- oligodeoxyribonukleotidy aplikace a dávkování imunologie MeSH
- tvorba protilátek účinky léků MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Stimulation with polyclonal activators is a tool to increase antibody secretion in B cells. The aim of the present study was to select the most effective common commercially available polyclonal activators of rabbit B cells. Specifically, type B oligodeoxynucleotides with unmethylated deoxycytidyl-deoxyguanosine dinucleotides (CpG-ODN), recombinant rabbit interleukin-2 (rrIL-2), lipopolysaccharide (LPS), pokeweed mitogen (PWM) and Resiquimod (R848) were tested on B cells isolated from blood and spleen by fluorescence-activated cell sorting. Based on the obtained data, stimulation with CpG-ODN induced the highest antigen-specific antibody levels detected by ELISA in supernatants when a single activator was used. In contrast, LPS, PWM and R848 showed a weak or no stimulatory effect. Stimulation with a mix of activators was more effective than CpG-ODN alone, which indicates a synergistic effect in the stimulation of antibody production.
Citace poskytuje Crossref.org
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