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A critical comparison of natural enzymes and nanozymes in biosensing and bioassays
AM. Ashrafi, Z. Bytesnikova, J. Barek, L. Richtera, V. Adam
Language English Country Great Britain
Document type Journal Article, Review
- MeSH
- Biosensing Techniques * MeSH
- Biological Assay MeSH
- Enzymes MeSH
- Catalysis MeSH
- Nanostructures * MeSH
- Publication type
- Journal Article MeSH
- Review MeSH
Nanozymes (NZs) are nanomaterials that mimic enzyme-like catalytic activity. They have attracted substantial attention due to their inherent physicochemical properties for use as promising alternatives to natural enzymes (NEs) in a variety of research fields. Particularly, in biosensing and bioassays, NZs have opened a new horizon to eliminate the intrinsic limitations of NEs, including their denaturation at extreme pH values and temperatures, poor reusability and recyclability, and high production costs. Moreover, the catalytic activity of NZs can be modulated in the preparation step by following an appropriate synthesis strategy. This review aims to gain insight into the potential substitution of NEs by NZs in biosensing and bioassays while considering both the pros and cons.
Central European Institute of Technology Brno University of Technology 612 00 Brno Czech Republic
Department of Chemistry and Biochemistry Mendel University in Brno CZ 613 00 Brno Czech Republic
References provided by Crossref.org
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- $a Ashrafi, Amir M $u Department of Chemistry and Biochemistry, Mendel University in Brno, CZ-613 00, Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, 612 00, Brno, Czech Republic
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- $a Nanozymes (NZs) are nanomaterials that mimic enzyme-like catalytic activity. They have attracted substantial attention due to their inherent physicochemical properties for use as promising alternatives to natural enzymes (NEs) in a variety of research fields. Particularly, in biosensing and bioassays, NZs have opened a new horizon to eliminate the intrinsic limitations of NEs, including their denaturation at extreme pH values and temperatures, poor reusability and recyclability, and high production costs. Moreover, the catalytic activity of NZs can be modulated in the preparation step by following an appropriate synthesis strategy. This review aims to gain insight into the potential substitution of NEs by NZs in biosensing and bioassays while considering both the pros and cons.
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