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Benefits and Pitfalls of HPLC Coupled to Diode-Array, Charged Aerosol, and Coulometric Detections: Effect of Detection on Screening of Bioactive Compounds in Apples
M. Hollá, A. Bílková, P. Jakubec, S. Košková, H. Kočová Vlčková, D. Šatínský, F. Švec, H. Sklenářová
Language English Country Switzerland
Document type Journal Article
Grant support
TJ02000196
Technologická Agentura České Republiky
1466119
Grantová Agentura, Univerzita Karlova
CZ.02.1.01/0.0/0.0/15_003/0000465
Ministerstvo Školství, Mládeže a Tělovýchovy
SVV 260 548
Univerzita Karlova v Praze
NLK
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- MeSH
- Aerosols chemistry MeSH
- Antioxidants chemistry MeSH
- Chromatography methods MeSH
- Electrochemistry methods MeSH
- Phenol chemistry MeSH
- Phenols analysis MeSH
- Calibration MeSH
- Limit of Detection MeSH
- Malus metabolism MeSH
- Food Technology MeSH
- Reproducibility of Results MeSH
- Chromatography, High Pressure Liquid methods MeSH
- Publication type
- Journal Article MeSH
The new screening method for rapid evaluation of major phenolic compounds in apples has been developed. Suitability of coupling HPLC/UHPLC separation with the diode-array detection and universal charged aerosol detection with respect to the presence of interfering substances was tested. Characteristics of both detection techniques were compared and method linearity, limits of detection and quantitation, and selectivity of them determined. Student t-test based on slopes of calibration plots was applied for the detailed comparison. The diode-array detection provided the best results regarding sensitivity and selectivity of the developed method in terms of evaluation of phenolics profiles. The response of the charged aerosol detector was negatively affected by co-eluting substances during rapid-screening analyses. Coulometric detection was used for advanced characterization of extracts in terms of antioxidant content and strength to obtain more complex information concerning sample composition. This detection also allowed evaluation of unidentified compounds with antioxidant activity. HPLC/UHPLC separation using a combination of diode-array and coulometric detectors thus represented the best approach enabling quick, yet complex characterization of bioactive compounds in apples.
References provided by Crossref.org
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- $a The new screening method for rapid evaluation of major phenolic compounds in apples has been developed. Suitability of coupling HPLC/UHPLC separation with the diode-array detection and universal charged aerosol detection with respect to the presence of interfering substances was tested. Characteristics of both detection techniques were compared and method linearity, limits of detection and quantitation, and selectivity of them determined. Student t-test based on slopes of calibration plots was applied for the detailed comparison. The diode-array detection provided the best results regarding sensitivity and selectivity of the developed method in terms of evaluation of phenolics profiles. The response of the charged aerosol detector was negatively affected by co-eluting substances during rapid-screening analyses. Coulometric detection was used for advanced characterization of extracts in terms of antioxidant content and strength to obtain more complex information concerning sample composition. This detection also allowed evaluation of unidentified compounds with antioxidant activity. HPLC/UHPLC separation using a combination of diode-array and coulometric detectors thus represented the best approach enabling quick, yet complex characterization of bioactive compounds in apples.
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