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S100A11 (calgizzarin) is released via NETosis in rheumatoid arthritis (RA) and stimulates IL-6 and TNF secretion by neutrophils
A. Navrátilová, V. Bečvář, J. Baloun, D. Damgaard, CH. Nielsen, D. Veigl, K. Pavelka, J. Vencovský, L. Šenolt, L. Andrés Cerezo
Jazyk angličtina Země Velká Británie
Typ dokumentu klinické zkoušky, časopisecké články, práce podpořená grantem
Free Medical Journals od 2011
Nature Open Access od 2011-12-01
PubMed Central od 2011
Europe PubMed Central od 2011
ProQuest Central od 2011-01-01
Open Access Digital Library od 2011-01-01
Open Access Digital Library od 2011-01-01
Health & Medicine (ProQuest) od 2011-01-01
ROAD: Directory of Open Access Scholarly Resources od 2011
Odkazy
PubMed
33727634
DOI
10.1038/s41598-021-85561-3
Knihovny.cz E-zdroje
- MeSH
- dospělí MeSH
- extracelulární pasti metabolismus MeSH
- interleukin-6 metabolismus MeSH
- lidé středního věku MeSH
- lidé MeSH
- neutrofily metabolismus patologie MeSH
- proteiny S100 metabolismus MeSH
- revmatoidní artritida metabolismus patologie MeSH
- senioři MeSH
- synoviální tekutina metabolismus MeSH
- TNF-alfa metabolismus MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- klinické zkoušky MeSH
- práce podpořená grantem MeSH
S100A11 (calgizzarin), a member of S100 family, is associated with several autoimmune diseases, including rheumatoid arthritis (RA). Neutrophil extracellular traps (NETs) are implicated in the pathogenesis of RA and in the externalization of some S100 family members. Therefore, we aimed to determine the association between S100A11 and NETs in RA. For this purpose, the levels of S100A11 and NETosis markers were detected in the RA synovial fluid by immunoassays. The expression of S100A11 by neutrophils in the RA synovial tissue was assessed. Neutrophils isolated from peripheral blood were exposed to S100A11 or stimulated to release NETs. The levels of NETosis- and inflammation-associated proteins were analysed by immunoassays. NETs were visualized by immunofluorescence. We showed that S100A11 was expressed by the neutrophils in the RA synovial tissue. Moreover, S100A11 in the RA synovial fluid correlated with several NETosis markers. In vitro, S100A11 was abundantly released by neutrophils undergoing NETosis compared to untreated cells (p < 0.001). Extracellular S100A11 increased the secretion of IL-6 (p < 0.05) and TNF (p < 0.05) by neutrophils but did not induce NETosis. This study demonstrates, for the first time, that the release of S100A11 is dependent on NETosis and that extracellular S100A11 augments the inflammatory response by inducing pro-inflammatory cytokines in neutrophils.
1st Orthopaedic Clinic 1st Faculty of Medicine Charles University Prague Czech Republic
Department of Rheumatology 1st Faculty of Medicine Charles University Prague Czech Republic
Institute of Rheumatology Na Slupi 4 12850 Prague Czech Republic
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