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Impact of electrolyte solution on electrochemical oxidation treatment of Escherichia coli K-12 by boron-doped diamond electrodes
J. Budil, O. Szabó, P. Lišková, P. Štenclová, T. Izsák, Š. Potocký, A. Kromka
Language English Country Great Britain
Document type Journal Article
Grant support
LM2018110
Ministerstvo Školství, Mládeže a Tělovýchovy
SOLID21-CZ.02.1.01/0.0/0.0/16_019/0000760
Ministerstvo Školství, Mládeže a Tělovýchovy
SVV 260568
Ministerstvo Školství, Mládeže a Tělovýchovy
PubMed
35239229
DOI
10.1111/lam.13687
Knihovny.cz E-resources
- MeSH
- Boron * chemistry pharmacology MeSH
- Sodium Chloride pharmacology MeSH
- Electrodes MeSH
- Electrolytes pharmacology MeSH
- Escherichia coli K12 * MeSH
- Escherichia coli MeSH
- Phosphates pharmacology MeSH
- Oxidation-Reduction MeSH
- Publication type
- Journal Article MeSH
We studied the disinfection efficacy of boron-doped electrodes on Escherichia coli-contaminated water-based solutions in three different electrolytes, physiological solution (NaCl), phosphate buffer (PB), and phosphate buffer saline (PBS). The effect of the electrochemical oxidation treatment on the bacteria viability was studied by drop and spread plate cultivation methods, and supported by optical density measurements. We have found that bacterial suspensions in NaCl and PBS underwent a total inactivation of all viable bacteria within 10 min of the electrochemical treatment. By contrast, experiments performed in the PB showed a relatively minor decrease of viability by two orders of magnitude after 2 h of the treatment, which is almost comparable with the untreated control. The enhanced bacterial inactivation was assigned to reactive chlorine species, capable of penetrating the bacterial cytoplasmic membrane and killing bacteria from within.
Department of Genetics and Microbiology Faculty of Science Charles University Prague Czech Republic
Institute of Electrical Engineering Slovak Academy of Sciences Bratislava Slovakia
Institute of Physics of the Czech Academy of Sciences Prague Czech Republic
References provided by Crossref.org
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