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Phylogenetic profiling resolves early emergence of PRC2 and illuminates its functional core
A. Sharaf, M. Vijayanathan, M. Oborník, I. Mozgová
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
Freely Accessible Science Journals od 2018
PubMed Central od 2018
ROAD: Directory of Open Access Scholarly Resources od 2018
Odkazy
PubMed
35440471
DOI
10.26508/lsa.202101271
Knihovny.cz E-zdroje
- MeSH
- fylogeneze MeSH
- histony genetika metabolismus MeSH
- lysin metabolismus MeSH
- PRC2 * genetika metabolismus MeSH
- proteiny Drosophily * metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Polycomb repressive complex 2 (PRC2) is involved in maintaining transcriptionally silent chromatin states through methylating lysine 27 of histone H3 by the catalytic subunit enhancer of zeste [E(z)]. Here, we report the diversity of PRC2 core subunit proteins in different eukaryotic supergroups with emphasis on the early-diverged lineages and explore the molecular evolution of PRC2 subunits by phylogenetics. For the first time, we identify the putative ortholog of E(z) in Discoba, a lineage hypothetically proximal to the eukaryotic root, strongly supporting emergence of PRC2 before the diversification of eukaryotes. Analyzing 283 species, we robustly detect a common presence of E(z) and ESC, indicating a conserved functional core. Full-length Su(z)12 orthologs were identified in some lineages and species only, indicating, nonexclusively, high divergence of VEFS-Box-containing Su(z)12-like proteins, functional convergence of sequence-unrelated proteins, or Su(z)12 dispensability. Our results trace E(z) evolution within the SET-domain protein family, proposing a substrate specificity shift during E(z) evolution based on SET-domain and H3 histone interaction prediction.
Biology Centre Czech Academy of Sciences Institute of Parasitology České Budějovice Czech Republic
Genetic Department Faculty of Agriculture Ain Shams University Cairo Egypt
University of South Bohemia Faculty of Science České Budějovice Czech Republic
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