• Something wrong with this record ?

Structure and function of the Arabidopsis ABC transporter ABCB19 in brassinosteroid export

W. Ying, Y. Wang, H. Wei, Y. Luo, Q. Ma, H. Zhu, H. Janssens, N. Vukašinović, M. Kvasnica, JM. Winne, Y. Gao, S. Tan, J. Friml, X. Liu, E. Russinova, L. Sun

. 2024 ; 383 (6689) : eadj4591. [pub] 20240322

Language English Country United States

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc24006678

Brassinosteroids are steroidal phytohormones that regulate plant development and physiology, including adaptation to environmental stresses. Brassinosteroids are synthesized in the cell interior but bind receptors at the cell surface, necessitating a yet to be identified export mechanism. Here, we show that a member of the ATP-binding cassette (ABC) transporter superfamily, ABCB19, functions as a brassinosteroid exporter. We present its structure in both the substrate-unbound and the brassinosteroid-bound states. Bioactive brassinosteroids are potent activators of ABCB19 ATP hydrolysis activity, and transport assays showed that ABCB19 transports brassinosteroids. In Arabidopsis thaliana, ABCB19 and its close homolog, ABCB1, positively regulate brassinosteroid responses. Our results uncover an elusive export mechanism for bioactive brassinosteroids that is tightly coordinated with brassinosteroid signaling.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc24006678
003      
CZ-PrNML
005      
20240423155435.0
007      
ta
008      
240412s2024 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1126/science.adj4591 $2 doi
035    __
$a (PubMed)38513023
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Ying, Wei $u Department of Neurology of The First Affiliated Hospital of USTC, MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, Hefei National Research Center for Physical Sciences at the Microscale, Biomedical Sciences and Health Laboratory of Anhui Province, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230027, China $1 https://orcid.org/0000000263739115
245    10
$a Structure and function of the Arabidopsis ABC transporter ABCB19 in brassinosteroid export / $c W. Ying, Y. Wang, H. Wei, Y. Luo, Q. Ma, H. Zhu, H. Janssens, N. Vukašinović, M. Kvasnica, JM. Winne, Y. Gao, S. Tan, J. Friml, X. Liu, E. Russinova, L. Sun
520    9_
$a Brassinosteroids are steroidal phytohormones that regulate plant development and physiology, including adaptation to environmental stresses. Brassinosteroids are synthesized in the cell interior but bind receptors at the cell surface, necessitating a yet to be identified export mechanism. Here, we show that a member of the ATP-binding cassette (ABC) transporter superfamily, ABCB19, functions as a brassinosteroid exporter. We present its structure in both the substrate-unbound and the brassinosteroid-bound states. Bioactive brassinosteroids are potent activators of ABCB19 ATP hydrolysis activity, and transport assays showed that ABCB19 transports brassinosteroids. In Arabidopsis thaliana, ABCB19 and its close homolog, ABCB1, positively regulate brassinosteroid responses. Our results uncover an elusive export mechanism for bioactive brassinosteroids that is tightly coordinated with brassinosteroid signaling.
650    _2
$a adenosintrifosfát $x metabolismus $7 D000255
650    12
$a Arabidopsis $x genetika $x metabolismus $7 D017360
650    12
$a proteiny huseníčku $x chemie $x genetika $x metabolismus $7 D029681
650    12
$a ABC transportéry $x chemie $x genetika $x metabolismus $7 D018528
650    12
$a brassinosteroidy $x metabolismus $7 D060406
650    _2
$a kyseliny indoloctové $x metabolismus $7 D007210
650    _2
$a konformace proteinů $7 D011487
655    _2
$a časopisecké články $7 D016428
700    1_
$a Wang, Yaowei $u Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium $u Center for Plant Systems Biology, VIB, 9052 Ghent, Belgium $1 https://orcid.org/0000000210012342
700    1_
$a Wei, Hong $u Department of Neurology of The First Affiliated Hospital of USTC, MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, Hefei National Research Center for Physical Sciences at the Microscale, Biomedical Sciences and Health Laboratory of Anhui Province, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230027, China $1 https://orcid.org/0009000181819004
700    1_
$a Luo, Yongming $u Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium $u Center for Plant Systems Biology, VIB, 9052 Ghent, Belgium $1 https://orcid.org/000000020474060X
700    1_
$a Ma, Qian $u Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium $u Center for Plant Systems Biology, VIB, 9052 Ghent, Belgium $1 https://orcid.org/0000000187180005
700    1_
$a Zhu, Heyuan $u Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium $u Center for Plant Systems Biology, VIB, 9052 Ghent, Belgium
700    1_
$a Janssens, Hilde $u Department of Organic and Macromolecular Chemistry, Ghent University, 9000 Ghent, Belgium $1 https://orcid.org/0000000167647181
700    1_
$a Vukašinović, Nemanja $u Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium $u Center for Plant Systems Biology, VIB, 9052 Ghent, Belgium $1 https://orcid.org/0000000327407874
700    1_
$a Kvasnica, Miroslav $u Laboratory of Growth Regulators, Institute of Experimental Botany, The Czech Academy of Sciences and Palacký University, 77900 Olomouc, Czech Republic $1 https://orcid.org/0000000205973261 $7 xx0117687
700    1_
$a Winne, Johan M $u Department of Organic and Macromolecular Chemistry, Ghent University, 9000 Ghent, Belgium $1 https://orcid.org/0000000290154497
700    1_
$a Gao, Yongxiang $u Department of Neurology of The First Affiliated Hospital of USTC, MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, Hefei National Research Center for Physical Sciences at the Microscale, Biomedical Sciences and Health Laboratory of Anhui Province, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230027, China $1 https://orcid.org/000000031214889X
700    1_
$a Tan, Shutang $u Department of Neurology of The First Affiliated Hospital of USTC, MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, Hefei National Research Center for Physical Sciences at the Microscale, Biomedical Sciences and Health Laboratory of Anhui Province, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230027, China $1 https://orcid.org/0000000204718285
700    1_
$a Friml, Jiří $u Institute of Science and Technology Austria (ISTA), 3400 Klosterneuburg, Austria $1 https://orcid.org/0000000283027596 $7 xx0212254
700    1_
$a Liu, Xin $u Department of Neurology of The First Affiliated Hospital of USTC, MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, Hefei National Research Center for Physical Sciences at the Microscale, Biomedical Sciences and Health Laboratory of Anhui Province, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230027, China $1 https://orcid.org/0000000283684369
700    1_
$a Russinova, Eugenia $u Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium $u Center for Plant Systems Biology, VIB, 9052 Ghent, Belgium $1 https://orcid.org/0000000205691977
700    1_
$a Sun, Linfeng $u Department of Neurology of The First Affiliated Hospital of USTC, MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, Hefei National Research Center for Physical Sciences at the Microscale, Biomedical Sciences and Health Laboratory of Anhui Province, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230027, China $1 https://orcid.org/0000000193672444
773    0_
$w MED00004282 $t Science $x 1095-9203 $g Roč. 383, č. 6689 (2024), s. eadj4591
856    41
$u https://pubmed.ncbi.nlm.nih.gov/38513023 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y - $z 0
990    __
$a 20240412 $b ABA008
991    __
$a 20240423155431 $b ABA008
999    __
$a ok $b bmc $g 2080970 $s 1216445
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2024 $b 383 $c 6689 $d eadj4591 $e 20240322 $i 1095-9203 $m Science $n Science $x MED00004282
LZP    __
$a Pubmed-20240412

Find record

Citation metrics

Archiving options