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Protocol for the induction of innate immune memory in human smooth muscle cells and endothelial cells in vitro

J. Sonnenberg, D. Schwarz, SM. Lagache, L. Schnack, H. Körner, M. Leffers, H. Hardege, Y. Liu, H. Reinecke, HM. Findeisen, Y. Sohrabi

. 2024 ; 5 (1) : 102776. [pub] 20231219

Language English Country United States

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc24006725

Non-immune cells, like innate immune cells, can develop a memory-like phenotype in response to priming with microbial compounds or certain metabolites, which enables an enhanced response to a secondary unspecific stimulus. This paper describes a step-by-step protocol for the induction and analysis of trained immunity in human endothelial and smooth muscle cells. We then describe steps for cell culture with cryopreserved vascular cells, subcultivation, and induction of trained immunity. We then provide detailed procedures for downstream analysis using ELISA and qPCR. For complete details on the use and execution of this protocol, please refer to Sohrabi et al. (2020)1 and Shcnack et al.2.

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$a Schwarz, Dennis $u Department of Cardiology I - Coronary and Peripheral Vascular Disease, Heart Failure, University Hospital Münster, Münster, Germany
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$a Schnack, Lucia $u Department of Cardiology I - Coronary and Peripheral Vascular Disease, Heart Failure, University Hospital Münster, Münster, Germany
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$a Körner, Helena $u Department of Cardiology I - Coronary and Peripheral Vascular Disease, Heart Failure, University Hospital Münster, Münster, Germany
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$a Leffers, Merle $u Department of Cardiology I - Coronary and Peripheral Vascular Disease, Heart Failure, University Hospital Münster, Münster, Germany
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$a Hardege, Hannah $u Department of Cardiology I - Coronary and Peripheral Vascular Disease, Heart Failure, University Hospital Münster, Münster, Germany
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$a Liu, Yuanyuan $u Department of Cardiology I - Coronary and Peripheral Vascular Disease, Heart Failure, University Hospital Münster, Münster, Germany
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$a Reinecke, Holger $u Department of Cardiology I - Coronary and Peripheral Vascular Disease, Heart Failure, University Hospital Münster, Münster, Germany
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$a Findeisen, Hannes M $u Department of Cardiology I - Coronary and Peripheral Vascular Disease, Heart Failure, University Hospital Münster, Münster, Germany
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$a Sohrabi, Yahya $u Department of Cardiology I - Coronary and Peripheral Vascular Disease, Heart Failure, University Hospital Münster, Münster, Germany; Department of Medical Genetics, Third Faculty of Medicine, Charles University, Prague, Czechia; Institutes of Molecular Genetics of the Czech Academy of Sciences, Prague, Czechia. Electronic address: yahya.sohrabi@ukmuenster.de
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