-
Je něco špatně v tomto záznamu ?
The correlation between human seminal plasma sialoproteins and ejaculate parameters
V. Palenikova, H. Pavlova, D. Kraus, Z. Kratka, K. Komrskova, P. Postlerova
Jazyk angličtina Země Nizozemsko
Typ dokumentu časopisecké články
- MeSH
- analýza spermatu metody MeSH
- apoptóza MeSH
- ejakulace MeSH
- glykodelin metabolismus MeSH
- glykoproteiny metabolismus MeSH
- klusterin metabolismus MeSH
- kyseliny sialové metabolismus MeSH
- laktoferrin metabolismus MeSH
- lektiny metabolismus chemie MeSH
- lidé MeSH
- motilita spermií MeSH
- proteiny semenné plazmy metabolismus MeSH
- sekreční proteiny semenných váčků metabolismus MeSH
- sperma * metabolismus chemie MeSH
- spermie * metabolismus MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
Sialic acids are negatively charged carbohydrates that are components of saccharide chains covalently linked to macromolecules. Sialylated glycoproteins are important for most biological processes, including reproduction, where they are associated with spermatogenesis, sperm motility, immune responses, and fertilization. Changes in the glycoprotein profile or sialylation in glycoproteins are likely to affect the quality of ejaculate. The aim of this study was to determine differences in the degree of sialylation between normozoospermic ejaculates and ejaculates with a pathological spermiogram using two lectins, Sambucus nigra (SNA) and Maackia amurensis (MAL II/MAA) recognizing α-2,6 or α-2,3 linkage of Sia to galactosyl residues. Our results show a close relationship between seminal plasma (SP) sialoproteins and the presence of anti-sperm antibodies in the ejaculate, apoptotic spermatozoa, and ejaculate quality. Using mass spectrometry, we identified SP sialoproteins such as, semenogelins, glycodelin, prolactin-inducible protein, lactotransferrin, and clusterin that are associated with spermatozoa and contribute to the modulation of the immune response and sperm apoptosis. Our findings suggest a correlation between the degree of SP glycoprotein sialylation and the existence of possible pathological states of spermatozoa and reproductive organs. Glycoproteins sialylation represents a potential parameter reflecting the overall quality of ejaculate and could potentially be utilised in diagnostics.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc24014093
- 003
- CZ-PrNML
- 005
- 20240905133419.0
- 007
- ta
- 008
- 240725s2024 ne f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.ijbiomac.2024.131341 $2 doi
- 035 __
- $a (PubMed)38574922
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a ne
- 100 1_
- $a Palenikova, Veronika $u Laboratory of Reproductive Biology, Institute of Biotechnology of the Czech Academy of Sciences, BIOCEV, Prumyslova 595, 252 50 Vestec, Czech Republic; Department of Biochemistry, Faculty of Science, Charles University, Hlavova 8, 128 40 Prague 2, Czech Republic. Electronic address: veronika.palenikova@ibt.cas.cz
- 245 14
- $a The correlation between human seminal plasma sialoproteins and ejaculate parameters / $c V. Palenikova, H. Pavlova, D. Kraus, Z. Kratka, K. Komrskova, P. Postlerova
- 520 9_
- $a Sialic acids are negatively charged carbohydrates that are components of saccharide chains covalently linked to macromolecules. Sialylated glycoproteins are important for most biological processes, including reproduction, where they are associated with spermatogenesis, sperm motility, immune responses, and fertilization. Changes in the glycoprotein profile or sialylation in glycoproteins are likely to affect the quality of ejaculate. The aim of this study was to determine differences in the degree of sialylation between normozoospermic ejaculates and ejaculates with a pathological spermiogram using two lectins, Sambucus nigra (SNA) and Maackia amurensis (MAL II/MAA) recognizing α-2,6 or α-2,3 linkage of Sia to galactosyl residues. Our results show a close relationship between seminal plasma (SP) sialoproteins and the presence of anti-sperm antibodies in the ejaculate, apoptotic spermatozoa, and ejaculate quality. Using mass spectrometry, we identified SP sialoproteins such as, semenogelins, glycodelin, prolactin-inducible protein, lactotransferrin, and clusterin that are associated with spermatozoa and contribute to the modulation of the immune response and sperm apoptosis. Our findings suggest a correlation between the degree of SP glycoprotein sialylation and the existence of possible pathological states of spermatozoa and reproductive organs. Glycoproteins sialylation represents a potential parameter reflecting the overall quality of ejaculate and could potentially be utilised in diagnostics.
- 650 _2
- $a mužské pohlaví $7 D008297
- 650 _2
- $a lidé $7 D006801
- 650 12
- $a sperma $x metabolismus $x chemie $7 D012661
- 650 12
- $a spermie $x metabolismus $7 D013094
- 650 _2
- $a motilita spermií $7 D013081
- 650 _2
- $a glykoproteiny $x metabolismus $7 D006023
- 650 _2
- $a glykodelin $x metabolismus $7 D000071816
- 650 _2
- $a sekreční proteiny semenných váčků $x metabolismus $7 D029607
- 650 _2
- $a analýza spermatu $x metody $7 D055101
- 650 _2
- $a klusterin $x metabolismus $7 D051152
- 650 _2
- $a lektiny $x metabolismus $x chemie $7 D037102
- 650 _2
- $a ejakulace $7 D004542
- 650 _2
- $a kyseliny sialové $x metabolismus $7 D012794
- 650 _2
- $a proteiny semenné plazmy $x metabolismus $7 D029589
- 650 _2
- $a laktoferrin $x metabolismus $7 D007781
- 650 _2
- $a apoptóza $7 D017209
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Pavlova, Hana $u Laboratory of Reproductive Biology, Institute of Biotechnology of the Czech Academy of Sciences, BIOCEV, Prumyslova 595, 252 50 Vestec, Czech Republic
- 700 1_
- $a Kraus, Daniel $u Laboratory of Gene Expression, Institute of Biotechnology of the Czech Academy of Sciences, BIOCEV, Prumyslova 595, 252 50 Vestec, Czech Republic
- 700 1_
- $a Kratka, Zuzana $u Gennet Archa Palace, Prague, Czech Republic
- 700 1_
- $a Komrskova, Katerina $u Laboratory of Reproductive Biology, Institute of Biotechnology of the Czech Academy of Sciences, BIOCEV, Prumyslova 595, 252 50 Vestec, Czech Republic; Department of Zoology, Faculty of Science, Charles University, BIOCEV, Vinicna 7, 128 44 Prague 2, Czech Republic
- 700 1_
- $a Postlerova, Pavla $u Laboratory of Reproductive Biology, Institute of Biotechnology of the Czech Academy of Sciences, BIOCEV, Prumyslova 595, 252 50 Vestec, Czech Republic; Department of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, University of Life Sciences Prague, Kamycka 129, 165 00 Prague 6, Czech Republic. Electronic address: pavla.postlerova@ibt.cas.cz
- 773 0_
- $w MED00002295 $t International journal of biological macromolecules $x 1879-0003 $g Roč. 266, č. Pt 2 (2024), s. 131341
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/38574922 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y - $z 0
- 990 __
- $a 20240725 $b ABA008
- 991 __
- $a 20240905133413 $b ABA008
- 999 __
- $a ok $b bmc $g 2143707 $s 1225959
- BAS __
- $a 3
- BAS __
- $a PreBMC-MEDLINE
- BMC __
- $a 2024 $b 266 $c Pt 2 $d 131341 $e 20240402 $i 1879-0003 $m International journal of biological macromolecules $n Int J Biol Macromol $x MED00002295
- LZP __
- $a Pubmed-20240725
