• Je něco špatně v tomto záznamu ?

Phosphonium chloride-based deep eutectic solvents inhibit pathogenic Acanthamoeba castellanii belonging to the T4 genotype

N. Akbar, AS. Khan, R. Siddiqui, TH. Ibrahim, MI. Khamis, BS. Alawfi, BM. Al-Ahmadi, NA. Khan

. 2025 ; 70 (1) : 101-113. [pub] 20240613

Jazyk angličtina Země Česko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc25006844

Herein, we investigated the anti-amoebic activity of phosphonium-chloride-based deep eutectic solvents against pathogenic Acanthamoeba castellanii of the T4 genotype. Deep eutectic solvents are ionic fluids composed of two or three substances, capable of self-association to form a eutectic mixture with a melting point lower than each substance. In this study, three distinct hydrophobic deep eutectic solvents were formulated, employing trihexyltetradecylphosphonium chloride as the hydrogen bond acceptor and aspirin, dodecanoic acid, and 4-tert-butylbenzoic acid as the hydrogen bond donors. Subsequently, all three deep eutectic solvents, denoted as DES1, DES2, DES3 formulations, underwent investigations comprising amoebicidal, adhesion, excystation, cytotoxicity, and cytopathogenicity assays. The findings revealed that DES2 was the most potent anti-amoebic agent, with a 94% elimination rate against the amoebae within 24 h at 30 °C. Adhesion assays revealed that deep eutectic solvents hindered amoebae adhesion to human brain endothelial cells, with DES2 exhibiting 88% reduction of adhesion. Notably, DES3 exhibited remarkable anti-excystation properties, preventing 94% of cysts from reverting to trophozoites. In cytopathogenicity experiments, deep eutectic solvent formulations and dodecanoic acid alone reduced amoebae-induced human brain endothelial cell death, with DES2 showing the highest effects. Lactate dehydrogenase assays revealed the minimal cytotoxicity of the tested deep eutectic solvents, with the exception of trihexyltetradecylphosphonium chloride, which exhibited 35% endothelial cell damage. These findings underscore the potential of specific deep eutectic solvents in combating pathogenic Acanthamoeba, presenting promising avenues for further research and development against free-living amoebae.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc25006844
003      
CZ-PrNML
005      
20250311100855.0
007      
ta
008      
250311s2025 xr f 000 0|eng||
009      
AR
024    7_
$a 10.1007/s12223-024-01180-1 $2 doi
035    __
$a (PubMed)38869777
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xr
100    1_
$a Akbar, Noor $u Research Institute of Medical and Health Sciences, University of Sharjah, 27272, Sharjah, United Arab Emirates. noor@gmail.com $u Department of Chemical Engineering, American University of Sharjah, P.O. Box 26666, Sharjah, United Arab Emirates. noor@gmail.com
245    10
$a Phosphonium chloride-based deep eutectic solvents inhibit pathogenic Acanthamoeba castellanii belonging to the T4 genotype / $c N. Akbar, AS. Khan, R. Siddiqui, TH. Ibrahim, MI. Khamis, BS. Alawfi, BM. Al-Ahmadi, NA. Khan
520    9_
$a Herein, we investigated the anti-amoebic activity of phosphonium-chloride-based deep eutectic solvents against pathogenic Acanthamoeba castellanii of the T4 genotype. Deep eutectic solvents are ionic fluids composed of two or three substances, capable of self-association to form a eutectic mixture with a melting point lower than each substance. In this study, three distinct hydrophobic deep eutectic solvents were formulated, employing trihexyltetradecylphosphonium chloride as the hydrogen bond acceptor and aspirin, dodecanoic acid, and 4-tert-butylbenzoic acid as the hydrogen bond donors. Subsequently, all three deep eutectic solvents, denoted as DES1, DES2, DES3 formulations, underwent investigations comprising amoebicidal, adhesion, excystation, cytotoxicity, and cytopathogenicity assays. The findings revealed that DES2 was the most potent anti-amoebic agent, with a 94% elimination rate against the amoebae within 24 h at 30 °C. Adhesion assays revealed that deep eutectic solvents hindered amoebae adhesion to human brain endothelial cells, with DES2 exhibiting 88% reduction of adhesion. Notably, DES3 exhibited remarkable anti-excystation properties, preventing 94% of cysts from reverting to trophozoites. In cytopathogenicity experiments, deep eutectic solvent formulations and dodecanoic acid alone reduced amoebae-induced human brain endothelial cell death, with DES2 showing the highest effects. Lactate dehydrogenase assays revealed the minimal cytotoxicity of the tested deep eutectic solvents, with the exception of trihexyltetradecylphosphonium chloride, which exhibited 35% endothelial cell damage. These findings underscore the potential of specific deep eutectic solvents in combating pathogenic Acanthamoeba, presenting promising avenues for further research and development against free-living amoebae.
650    12
$a Acanthamoeba castellanii $x účinky léků $x genetika $7 D048248
650    _2
$a lidé $7 D006801
650    12
$a rozpouštědla $x chemie $x farmakologie $7 D012997
650    12
$a genotyp $7 D005838
650    _2
$a endoteliální buňky $x účinky léků $7 D042783
650    _2
$a buněčná adheze $x účinky léků $7 D002448
650    _2
$a amébicidy $x farmakologie $x chemie $7 D000563
650    _2
$a organofosforové sloučeniny $x farmakologie $x chemie $7 D009943
650    _2
$a trofozoiti $x účinky léků $7 D053058
655    _2
$a časopisecké články $7 D016428
700    1_
$a Khan, Amir Sada $u Department of Chemical Engineering, American University of Sharjah, P.O. Box 26666, Sharjah, United Arab Emirates $u Department of Chemistry, University of Science and Technology Bannu, Bannu, 28100, Khyber Pakhtunkhwa, Pakistan
700    1_
$a Siddiqui, Ruqaiyyah $u Institute of Biological Chemistry, Biophysics and Bioengineering, Heriot-Watt University Edinburgh, Edinburgh, EH14 4AS, UK $u Microbiota Research Center, Istinye University, Istanbul, 34010, Turkey
700    1_
$a Ibrahim, Taleb Hassan $u Department of Chemical Engineering, American University of Sharjah, P.O. Box 26666, Sharjah, United Arab Emirates
700    1_
$a Khamis, Mustafa I $u College of Arts and Sciences, American University of Sharjah, 26666, Sharjah, United Arab Emirates
700    1_
$a Alawfi, Bader S $u Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Taibah University, Medina, Saudi Arabia
700    1_
$a Al-Ahmadi, Bassam M $u Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Taibah University, Medina, Saudi Arabia
700    1_
$a Khan, Naveed Ahmed $u Microbiota Research Center, Istinye University, Istanbul, 34010, Turkey. naveed5438@gmail.com $1 https://orcid.org/0000000176678553
773    0_
$w MED00011005 $t Folia microbiologica $x 1874-9356 $g Roč. 70, č. 1 (2025), s. 101-113
856    41
$u https://pubmed.ncbi.nlm.nih.gov/38869777 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y - $z 0
990    __
$a 20250311 $b ABA008
991    __
$a 20250311100904 $b ABA008
999    __
$a ok $b bmc $g 2282756 $s 1243909
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2025 $b 70 $c 1 $d 101-113 $e 20240613 $i 1874-9356 $m Folia microbiologica $n Folia Microbiol (Praha) $x MED00011005
LZP    __
$a Pubmed-20250311

Najít záznam

Citační ukazatele

Možnosti archivace