Role of membrane cholesterol in differential sensitivity of muscarinic receptor subtypes to persistently bound xanomeline
Jazyk angličtina Země Anglie, Velká Británie Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
29407765
DOI
10.1016/j.neuropharm.2018.01.027
PII: S0028-3908(18)30027-3
Knihovny.cz E-zdroje
- Klíčová slova
- Cholesterol (PubChem CID: 5997), Membrane cholesterol, Methyl beta-cyclodextrin (PubChem CID: 51051622), Molecular dynamics, Muscarinic acetylcholine receptors, N-methyl scopolamine (PubChem CID: 5459110), Receptor activation, Xanomeline, Xanomeline (Pubchem CID: 60809),
- MeSH
- agonisté muskarinových receptorů farmakokinetika MeSH
- buněčná membrána účinky léků metabolismus MeSH
- CHO buňky cytologie MeSH
- cholesterol metabolismus MeSH
- Cricetulus MeSH
- inositolfosfáty metabolismus MeSH
- magnetická rezonanční spektroskopie MeSH
- molekulární modely MeSH
- průtoková cytometrie MeSH
- pyridiny farmakokinetika MeSH
- radioligandová zkouška MeSH
- receptory muskarinové genetika metabolismus MeSH
- simulace molekulového dockingu MeSH
- thiadiazoly farmakokinetika MeSH
- tritium farmakokinetika MeSH
- vápník metabolismus MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- agonisté muskarinových receptorů MeSH
- cholesterol MeSH
- inositolfosfáty MeSH
- pyridiny MeSH
- receptory muskarinové MeSH
- thiadiazoly MeSH
- tritium MeSH
- vápník MeSH
- xanomeline MeSH Prohlížeč
Xanomeline (3-(Hexyloxy)-4-(1-methyl-1,2,5,6-tetrahydropyridin-3-yl)-1,2,5-thiadiazole) is a muscarinic agonist that is considered to be functionally selective for the M1/M4 receptor subtypes. Part of xanomeline binding is resistant to washing. Wash-resistant xanomeline activates muscarinic receptors persistently, except for the M5 subtype. Mutation of leucine 6.46 to isoleucine at M1 or M4 receptors abolished persistent activation by wash-resistant xanomeline. Reciprocal mutation of isoleucine 6.46 to leucine at the M5 receptor made it sensitive to activation by wash-resistant xanomeline. Lowering of membrane cholesterol made M1 and M4 mutants and M5 wild type receptors sensitive to activation by wash-resistant xanomeline. Molecular docking revealed a cholesterol binding site in the groove between transmembrane helices 6 and 7. Molecular dynamics showed that interaction of cholesterol with this binding site attenuates receptor activation. We hypothesize that differences in cholesterol binding to this site between muscarinic receptor subtypes may constitute the basis for xanomeline apparent functional selectivity and may have notable therapeutic implications. Differences in receptor-membrane interactions, rather than in agonist-receptor interactions, represent a novel possibility to achieve pharmacological selectivity. Our findings may be applicable to other G protein coupled receptors.
Citace poskytuje Crossref.org
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