• Je něco špatně v tomto záznamu ?

Molecular identification of lactic acid bacteria from traditional fermented foods and screening exopolysaccharide production by using food wastes

K. Karaman, ST. Sirke, ŞNT. Rifaioglu

. 2025 ; 70 (2) : 391-401. [pub] 20240827

Jazyk angličtina Země Česko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc25013016

Grantová podpora
FOA-2021-10953 Bilimsel Araştırma Projeleri, Erciyes Üniversitesi

In this study, lactic acid bacteria (LAB) isolation from fermented foods and molecular identification using magnetic bead technology were performed. And then exopolysaccharide (EPS) production possibility was tested in agar medium, and the positive ones were selected for the next step. The bacteria that could produce higher carbohydrate level were grown in MRS medium fortified with whey and pumpkin waste. In our study, 19 different LAB species were identified from fermented products collected from different places in Hatay (Türkiye) province. In molecular identification, universal primer pairs, p806R/p8FPL, and PEU7/DG74 were used for PCR amplification. After that, PCR products purified using paramagnetic bead technology were sequenced by the Sanger sequencing method. The dominant species, 23.8% of the isolates, were identified as Lactiplantibacillus plantarum. As a technological property of LAB, exopolysaccharide production capability of forty-two LAB isolate was tested in agar medium, and after eleven isolates were selected as positive. Two LAB (Latilactobacillus curvatus SHA2-3B and Loigolactobacillus coryniformis SHA6-3B) had higher EPS production capability when they were grown in MRS broth fortified with pumpkin waste and whey. The highest EPS content (1750 mg/L glucose equivalent) was determined in Loigolactobacillus coryniformis SHA6-3B grown in MRS broth fortified with 10% pumpkin waste. Besides the produced EPS samples were validated with FTIR and SEM methods.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc25013016
003      
CZ-PrNML
005      
20250605092910.0
007      
ta
008      
250605s2025 xr f 000 0|eng||
009      
AR
024    7_
$a 10.1007/s12223-024-01187-8 $2 doi
035    __
$a (PubMed)39192167
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xr
100    1_
$a Karaman, Kevser $u Department of Agricultural Biotechnology, Faculty of Agriculture, Erciyes University, Kayseri, Türkiye. kevserkaraman@erciyes.edu.tr $u Genome and Stem Cell Research Center, Erciyes University, Kayseri, Türkiye. kevserkaraman@erciyes.edu.tr $1 https://orcid.org/0000000307296185
245    10
$a Molecular identification of lactic acid bacteria from traditional fermented foods and screening exopolysaccharide production by using food wastes / $c K. Karaman, ST. Sirke, ŞNT. Rifaioglu
520    9_
$a In this study, lactic acid bacteria (LAB) isolation from fermented foods and molecular identification using magnetic bead technology were performed. And then exopolysaccharide (EPS) production possibility was tested in agar medium, and the positive ones were selected for the next step. The bacteria that could produce higher carbohydrate level were grown in MRS medium fortified with whey and pumpkin waste. In our study, 19 different LAB species were identified from fermented products collected from different places in Hatay (Türkiye) province. In molecular identification, universal primer pairs, p806R/p8FPL, and PEU7/DG74 were used for PCR amplification. After that, PCR products purified using paramagnetic bead technology were sequenced by the Sanger sequencing method. The dominant species, 23.8% of the isolates, were identified as Lactiplantibacillus plantarum. As a technological property of LAB, exopolysaccharide production capability of forty-two LAB isolate was tested in agar medium, and after eleven isolates were selected as positive. Two LAB (Latilactobacillus curvatus SHA2-3B and Loigolactobacillus coryniformis SHA6-3B) had higher EPS production capability when they were grown in MRS broth fortified with pumpkin waste and whey. The highest EPS content (1750 mg/L glucose equivalent) was determined in Loigolactobacillus coryniformis SHA6-3B grown in MRS broth fortified with 10% pumpkin waste. Besides the produced EPS samples were validated with FTIR and SEM methods.
650    12
$a bakteriální polysacharidy $x biosyntéza $x metabolismus $7 D011135
650    12
$a Lactobacillales $x izolace a purifikace $x klasifikace $x genetika $x metabolismus $7 D056546
650    12
$a fermentované potraviny $x mikrobiologie $7 D000074421
650    12
$a potravinářská mikrobiologie $7 D005516
650    _2
$a fermentace $7 D005285
650    12
$a odpadní produkty $x analýza $7 D014866
650    _2
$a kultivační média $x chemie $7 D003470
650    _2
$a RNA ribozomální 16S $x genetika $7 D012336
650    _2
$a fylogeneze $7 D010802
650    _2
$a Cucurbita $x mikrobiologie $7 D028464
650    _2
$a syrovátka $7 D000067796
655    _2
$a časopisecké články $7 D016428
700    1_
$a Sirke, Sibel Turan $u Department of Agricultural Biotechnology, Faculty of Agriculture, Van Yuzuncuyil University, Van, Türkiye $u Graduate School of Natural and Applied Sciences, Erciyes University, Kayseri, Türkiye
700    1_
$a Rifaioglu, Şeyda Nur Türkay $u Genome and Stem Cell Research Center, Erciyes University, Kayseri, Türkiye $u Graduate School of Natural and Applied Sciences, Erciyes University, Kayseri, Türkiye
773    0_
$w MED00011005 $t Folia microbiologica $x 1874-9356 $g Roč. 70, č. 2 (2025), s. 391-401
856    41
$u https://pubmed.ncbi.nlm.nih.gov/39192167 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y - $z 0
990    __
$a 20250605 $b ABA008
991    __
$a 20250605092903 $b ABA008
999    __
$a ok $b bmc $g 2333593 $s 1250126
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2025 $b 70 $c 2 $d 391-401 $e 20240827 $i 1874-9356 $m Folia microbiologica $n Folia Microbiol (Praha) $x MED00011005
GRA    __
$a FOA-2021-10953 $p Bilimsel Araştırma Projeleri, Erciyes Üniversitesi
LZP    __
$a Pubmed-20250605

Najít záznam

Citační ukazatele

Možnosti archivace