Membrane vesicles obtained from Acholeplasma laidlawii accumulate glucose as well as maltose and fructose against their concentration gradient in the absence of exogenous energy sources. Glucose uptake by membrane vesicles is inhibited by anaerobiosis and by electron transfer inhibitors, such as rotenone and amytal, but not by 2-heptyl-4-hydroxyquinoline N-oxide, antimycin A, cyanide and azide. Rotenone, cyanide and amytal also produce a rapid efflux of glucose from the membrane vesicles. Arsenate, oligomycin and N,N'-dicyclohexylcarbodimide do not inhibit glucose transport. Transport of glucose is markedly inhibited by proton conductors such as CCCP and pentachlorophenol. It is concluded that glucose transport can be driven by a high-energy state of the membrane or by the membrane potential.

See more in PubMed

Biochim Biophys Acta. 1969 Jul 15;183(2):295-303 PubMed

J Bioenerg. 1973 Jan;4(1):63-91 PubMed

Arch Biochem Biophys. 1974 Jan;160(1):215-22 PubMed

Ann N Y Acad Sci. 1974 Feb 18;227:297-311 PubMed

Biochem Biophys Res Commun. 1972 May 26;47(4):838-45 PubMed

Folia Microbiol (Praha). 1975;20(6):470-9 PubMed

Biochim Biophys Acta. 1973 Oct 25;323(3):429-40 PubMed

Biochem Biophys Res Commun. 1973 Jan 23;50(2):551-8 PubMed

Ann N Y Acad Sci. 1974 Feb 18;227:312-27 PubMed

J Biol Chem. 1973 May 25;248(10):3551-65 PubMed

J Biol Chem. 1972 Nov 25;247(22):7257-65 PubMed

Folia Microbiol (Praha). 1975;20(6):480-7 PubMed

Eur J Biochem. 1973 Dec 3;40(1):171-5 PubMed

Proc Natl Acad Sci U S A. 1973 Dec;70(12):3376-81 PubMed