Effect of detoxification processes on the interferon-inducing activity of bacterial endotoxins
Jazyk angličtina Země Maďarsko Médium print
Typ dokumentu srovnávací studie, časopisecké články, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.
Grantová podpora
HL 31035
NHLBI NIH HHS - United States
PubMed
2484274
Knihovny.cz E-zdroje
- MeSH
- acetylace MeSH
- endotoxiny izolace a purifikace farmakologie MeSH
- Escherichia coli metabolismus MeSH
- hydrolýza MeSH
- interferony biosyntéza krev MeSH
- králíci MeSH
- Limulus test MeSH
- lipopolysacharidy izolace a purifikace fyziologie MeSH
- metabolická inaktivace * MeSH
- Salmonella metabolismus MeSH
- Shigella metabolismus MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
- Research Support, U.S. Gov't, P.H.S. MeSH
- srovnávací studie MeSH
- Názvy látek
- endotoxiny MeSH
- interferony MeSH
- lipopolysacharidy MeSH
The interferon (IFN)-inducing activity of detoxified lipopolysaccharide (LPS) was tested in rabbits treated with LPS preparation derived from Escherichia coli, Salmonella typhi, Salmonella enteritidis and Shigella dysenteriae serovar 1. Of the detoxification procedures used, alkaline hydrolysis, hydroxylaminolysis, formalization, treatment with sodium deoxycholate and the radiodetoxification (fast or slow) methods had no appreciable effects on the IFN-inducing potential of LPS. In contrast, acetylation or prolonged alkaline hydrolysis of LPS resulted in up to a 9-fold reduction of IFN-induction capacity and effects of Cu++ or Fe++ cations bound to LPS were clearly inhibitory (Fe more than Cu).
