Quinolones and coumarins eliminate chloroplasts from Euglena gracilis
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
2558612
PubMed Central
PMC172782
DOI
10.1128/aac.33.11.1883
Knihovny.cz E-zdroje
- MeSH
- 4-chinolony MeSH
- antiinfekční látky farmakologie MeSH
- chloramfenikol farmakologie MeSH
- chloroplasty účinky léků MeSH
- elektronová mikroskopie MeSH
- Euglena gracilis účinky léků ultrastruktura MeSH
- inhibitory topoisomerasy II MeSH
- koncentrace vodíkových iontů MeSH
- kumariny farmakologie MeSH
- rifampin farmakologie MeSH
- teplota MeSH
- tma MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- 4-chinolony MeSH
- antiinfekční látky MeSH
- chloramfenikol MeSH
- inhibitory topoisomerasy II MeSH
- kumariny MeSH
- rifampin MeSH
Quinolones and coumarins were potent eliminators of chloroplasts from Euglena gracilis. There was a remarkable similarity between antichloroplastic and antibacterial activities of DNA gyrase inhibitors. Quinolones produced 100% chloroplast-free cells in concentrations which do not affect cell viability. Optimal conditions were exponential growth, continuous illumination, and neutral or slightly alkaline pH. Coumarins were more toxic than quinolones. Among the quinolones, ofloxacin was the most potent in eliminating chloroplasts. Among the coumarins, coumermycin A1 was the most potent. New quinolones and coumermycin A1 were able to induce the complete inability of originally green cells to form green colonies after 24 h of drug exposure, while clorobiocin and novobiocin required several days of exposure. Darkness, heat shock (42 degrees C, 10 min), or simultaneous treatment with chloramphenicol or rifampin decreased the potency of DNA gyrase inhibitors for producing chloroplast-free cells. Remarkably, in cells in which division was blocked by three different methods (resting medium, hyperthermic conditions [37 degrees C], or addition of cycloheximide), new quinolones and coumermycin A1 nevertheless eliminated chloroplasts. The antichloroplastic activity of DNA gyrase inhibitors is additional data suggesting an evolutionary relationship between chloroplasts and eubacteria.
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