Fluorescent staining with bromocresol purple: a rapid method for determining yeast cell dead count developed as an assay of killer toxin activity
Language English Country Great Britain, England Media print
Document type Comparative Study, Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
7509098
DOI
10.1002/yea.320091107
Knihovny.cz E-resources
- MeSH
- Staining and Labeling methods MeSH
- Bromcresol Purple * MeSH
- Cell Membrane drug effects MeSH
- Fluorescent Dyes MeSH
- Killer Factors, Yeast MeSH
- Mycotoxins analysis pharmacology MeSH
- Colony Count, Microbial methods MeSH
- Saccharomyces cerevisiae Proteins MeSH
- Saccharomyces cerevisiae cytology drug effects MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Bromcresol Purple * MeSH
- Fluorescent Dyes MeSH
- Killer Factors, Yeast MeSH
- Mycotoxins MeSH
- Saccharomyces cerevisiae Proteins MeSH
A method is described for detecting yeast cells with plasma membrane damage, based on cell staining with bromocresol purple (BCP) which has a convenient fluorescence after entering the cells at pH below 5.2. The method was used to determine the activity of Saccharomyces cerevisiae pore-forming killer toxin K1 in commonly used lethal units. The BCP test is rapid and as precise as the plating test.
References provided by Crossref.org
General and molecular microbiology and microbial genetics in the IM CAS
Yeast killer toxin K1 and its exploitation in genetic manipulations
Significance of the lag phase in K1 killer toxin action on sensitive yeast cells
Kinetic studies of killer toxin K1 binding to yeast cells indicate two receptor populations
Study of membrane potential changes of yeast cells caused by killer toxin K1
Factors affecting the susceptibility of sensitive yeast cells to killer toxin K1
