Water-soluble poly(acrylamide-allylamine) derivatives of saccharides for protein-saccharide binding studies
Language English Country United States Media print
Document type Journal Article
PubMed
7795412
DOI
10.1007/bf00731868
Knihovny.cz E-resources
- MeSH
- Acrylic Resins chemistry MeSH
- Allylamine chemistry MeSH
- Lectins chemistry metabolism MeSH
- Ligands MeSH
- Polymers chemistry MeSH
- Polysaccharides chemistry metabolism MeSH
- Proteins chemistry metabolism MeSH
- Solubility MeSH
- Water chemistry MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Acrylic Resins MeSH
- Allylamine MeSH
- Lectins MeSH
- Ligands MeSH
- polyacrylamide MeSH Browser
- Polymers MeSH
- Polysaccharides MeSH
- Proteins MeSH
- Water MeSH
Water-soluble poly(acrylamide-allylamine) copolymers containing covalently bound amino groups, prepared by copolymerization of acrylamide and allylamine, can be used as general carriers for coupling of different types of saccharides or saccharide derivatives. The water-soluble macromolecular carbohydrate derivatives can be easily labelled and used in various solid-phase techniques to study protein-saccharide interaction. Two types of coupling reaction were used to prepare polyacrylamide derivatives of saccharides: reductive amination was applied to couple the reducing disaccharides and a carbodiimide reaction was used to couple heparin via its carboxyl groups to the amino groups of the poly(acrylamide-allylamine) derivative. Peroxidase labelled or biotinylated derivatives were shown to be useful in studies on the binding properties of lectins and proteins from boar seminal plasma.
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